Immuno-fluorescence of Aldolase b (Thermo-Fisher, cat: PA5-30218) and Glutamine Synthetase (Thermo-Fisher, cat: MA5-27749) was perform in similar method to E-cadherin and CD73, however to these primary antibodies, secondary antibodies were needed – after overnight incubation with the primary antibodies, slides were incubated 2h at room temperature with secondary antibodies prior to dapi stain. For Aldob antibody we used 1:500 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody conjugated to Cy3 (Thermo-Fisher, cat: A10520). For GS antibody we used 1:500 Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody conjugated to Cy3 (Thermo-Fisher, cat: A10521).
Goat anti rabbit igg h l cross adsorbed secondary antibody conjugated to cy3
Manufactured by Thermo Fisher Scientific
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody conjugated to Cy3 is a secondary antibody used in immunoassays and other applications to detect the presence of rabbit primary antibodies. The antibody is conjugated to the fluorescent dye Cy3, allowing for visualization and detection of target proteins or other biomolecules.
Automatically generated - may contain errors
2 protocols using goat anti rabbit igg h l cross adsorbed secondary antibody conjugated to cy3
1
Immunofluorescence of Aldolase b and Glutamine Synthetase
2
Immunofluorescence of Aldolase b and Glutamine Synthetase
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