Transgenic mice expressingGCaMP2.2c inL5 pyramidal neurons, Thy1-GCaMP2.2c (ref. 44 (link)), were received from G. Feng. Adeno-associated virus (AAV) experiments were conducted with C57/BL6 mice (Charles River Laboratory) and Thy1-Cre transgenic mice (FVB/N-Tg (Thy1-Cre)1 Vln/J; the Jackson Laboratories). SST-IRES-Cre mice (Ssttm2.1(cre)Zjh/J) and Rosa26-stop-iDTR mice were purchased from the Jackson Laboratories. Mice were housed in the Parasitology Central Animal Facility at New York University Medical Center. All of the 1–1.5-month-old male and female animals were used for the experiments in accordance with the Institutional Guidelines.
Sst ires cre mice ssttm2.1 cre zjh j
Manufactured by Jackson ImmunoResearch
The SST-IRES-Cre mice (Ssttm2.1(cre)Zjh/J) are a genetically modified mouse strain that expresses Cre recombinase under the control of the somatostatin (Sst) gene promoter. This allows for the selective targeting and manipulation of somatostatin-expressing cells in the mouse model.
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2 protocols using sst ires cre mice ssttm2.1 cre zjh j
1
Transgenic Mouse Experiments with GCaMP2.2c
2
In Vivo Imaging of Transgenic Mice
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The wild-type (WT) C57BL/6 mice and transgenic mice Thy1-YFP in layer 5 pyramidal neurons (H line), Thy1-eGFP in pyramidal neurons (M line), Cx3cr1-GFP mice expressing eGFP in microglia cell (Cx3cr1 tm1Litt /J), and SST-IRES-Cre mice (Sst tm2.1(cre) Zjh /J) were purchased from the Jackson Laboratories. Mice were housed in Bindley Bioscience Center Animal Facility at Purdue University. All the one-or two-months old animal procedures were performed under the institutional guidelines.
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