IHC assays were performed on formalin-fixed paraffin-embedded (FFPE) tissue samples. These assays were carried out according to the manufacturer recommendations on an automated IHC stainer Bond RX (Leica Biosystems, Buffalo Grove, IL, USA). IHC analysis was performed against Ki-67 [Biocare Medical; CRM325B, rabbit monoclonal, dilution 1/150], using the BOND Epitope Retrieval Solution 1 (ER1; citrate based, pH 6) for 20 min for heat-induced Epitope Retrieval. Sections were incubated with 150 µl of antibody at RT for 30/15 min (primary/secondary antibody, respectively). Detection of specific signal was acquired by using Bond Polymer Refine Detection System containing the DAB chromogen (#DS9800, Leica Biosystems). Stained slides were scanned using the NanoZoomer Digital Pathology (NDP) 2.0-HT digital slide scanner (Hamamatsu, Japan). IHC quantifications were performed in 4 tumors/group.
Nanozoomer digital pathology 2.0 ht digital slide scanner
Manufactured by Hamamatsu Photonics
Sourced in Japan
The NanoZoomer Digital Pathology (NDP) 2.0-HT is a digital slide scanner developed by Hamamatsu Photonics. The core function of this equipment is to digitize microscopic slides, converting them into high-resolution digital images that can be viewed, shared, and analyzed on a computer.
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Lab products found in correlation
2 protocols using nanozoomer digital pathology 2.0 ht digital slide scanner
1
Automated IHC Staining of Ki-67 in FFPE Samples
2
Digital Slide Scanning for Tissue Microarray Analysis
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The stained slides were next subjected to digital slide scanning that converts glass slides into high-resolution digital data by high-speed scanning using the NanoZoomer Digital Pathology (NDP) 2.0-HT digital slide scanner (Hamamatsu, Japan).Using digital microscopy for scoring of scanned TMA images has advantages over the conventional light microscopic method. These include ease of handling and linking of cores to the predefined TMA “map” which ensures that each core/case is accurately identified and recorded. Moreover, the samples can be accessed and evaluated via any computer without the requirement for availability of a conventional light microscope [40] (link).
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