Cells were plated at 1 × 106 per 100 mm dish and treated with 50 μM riluzole or vehicle (dimethyl sulfoxide) for 24–48 h. Adherent and floating cells were pooled, pelleted, washed twice with ice-cold 1X PBS, and fixed by dropwise addition of ice-cold 70 % ethanol while mixing and stored at 20 °C. Fixed cells were washed twice with and resuspended in 1X PBS, treated with RNase A solution (Sigma, St. Louis, MO) at 100 mg/ml and stained with propidium iodide (Sigma, St Louis, MO) at 10 mg/ml for 30 min. Cell cycle analysis was performed on a Coulter Cytomics FC500 Flow Cytometer (Beckman Coulter, Fullerton, CA, USA) at the Analytical Cytometry Core Facility, Rutgers University.
Coulter cytomics fc500 flow cytometer
Manufactured by Beckman Coulter
Sourced in United States
The Coulter Cytomics FC500 Flow Cytometer is a laboratory instrument designed for the analysis and sorting of cells and particles. It utilizes flow cytometry technology to detect and measure the physical and fluorescent characteristics of individual cells or particles passing through a laser beam. The core function of the FC500 is to provide quantitative data on the size, granularity, and fluorescent labeling of cell samples.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by Merck Group (1 mentions)
Rnase a solution, by Merck Group (1 mentions)
Cd45 fitc, by Beckman Coulter (1 mentions)
Cd133 pe, by Miltenyi Biotec (1 mentions)
Cd133 apc, by Miltenyi Biotec (1 mentions)
Cd5 ecd, by Beckman Coulter (1 mentions)
Cd34 fitc, by Beckman Coulter (1 mentions)
Cd3 pe, by Beckman Coulter (1 mentions)
Cd45 pc7, by Beckman Coulter (1 mentions)
Cxp software, by Beckman Coulter (1 mentions)
Fitc brdu flow kit, by BD (1 mentions)
3 protocols using coulter cytomics fc500 flow cytometer
1
Cell Cycle Analysis of Riluzole Treatment
2
Flow Cytometry Immunophenotyping Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies directed against the following human cell surface proteins were obtained from commercial sources: CD45-PC7, CD45-FITC, CD3-PE, CD5-ECD, CD34-FITC, CD19-APC, CD23-PE (Beckman Coulter, Miami, FL) CD133-PE and CD133-APC (Miltenyi Biotec, Auburn, CA). Stained samples were washed in PBS containing 2% FBS and 7-aminoactinomycin (7-AAD). A minimum of 20,000 cells (unless noted otherwise) was analyzed on a Coulter Cytomics FC500 flow cytometer (Beckman Coulter, Miami, FL). All analysis of normal and lymphoma cells were gated on human CD45+ cells. Sequential gates were set to include only viable cells and quadrant markers were set to exclude at least 99.9% of cells labeled with the appropriate fluorochrome labeled isotype controls.
3
Rapamycin Modulates PGRN-Induced Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa cells were treated with DMSO or 100 μM rapamycin for 1 h, then with PBS or 500 ng/mL rhPGRN for 6 h, and BrdU labeling was assessed by use of the FITC BrdU Flow Kit (BD Biosciences, San Diego, CA). Flow cytometric analysis was performed using a Coulter cytomics FC500 flow cytometer (Beckman Coulter, Fullerton, CA) with CXP software (Beckman Coulter).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!