Ricin b chain

Manufactured by Vector Laboratories

Ricin B chain is a component of the ricin toxin, which is derived from the seeds of the castor bean plant. The B chain is responsible for binding the toxin to target cells, facilitating its entry into the cell. This protein has applications in research and analytical procedures, but its specific intended uses should be determined by the end user.

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2 protocols using ricin b chain

Ricin Purification and Mass Spectrometry

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Ricin (communis agglutinin II) and ricin B chain were obtained commercially from Vector Laboratories (Burlingame, CA). Matrixassisted laser desorption/ionization-time-of-flight matrix α-cyano-4hydroxycinnamic acid (CHCA) was purchased from Sigma (St. Louis, MO). Glacial acetic acid, water, trifluoroacetic acid, formic acid, and acetonitrile were purchased from Fisher Chemical (Fair Lawn, NJ).

Chen D., Bryden W.A, & Fenselau C. (2018). Rapid analysis of ricin using hot acid digestion and MALDI-TOF mass spectrometry. Journal of mass spectrometry : JMS, 53(10), 1013-1017.

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Ricin B-chain Purification and Detection

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Ricin B-chain was obtained from Vector Labs (Burlingame, CA), HEPES, α-lactose monohydrate, trypsin, pronase, bafilomycin A1, pepstatin A and CA074 methyl ester came from Sigma-Aldrich (St. Louis, MO). [³H]Leucine was purchased from GE Healthcare (Princeton, NJ), Na₂³⁵SO₄ and L-[³⁵S]Methionine came from Hartmann Analytic (Braunschweig, Germany). The mouse monoclonal anti-HA antibodies were obtained from Covance Research Products (Denver, CO), rabbit anti-BACE from Merck (Whitehouse Station, NJ), rabbit anti-Ricinus Communis-Lectin, and mouse anti-α-tubulin came from Sigma-Aldrich, whereas mouse monoclonal anti-ricin A-chain were purchased from Serotec (Oxford, UK). The mouse anti-calnexin were obtained from BD Biosciences (Palo Alto, CA), anti-calreticulin from BioSite (San Diego, CA). The rabbit anti-His antibodies came from Santa Cruz Biotechnology (Santa Cruz, CA). The secondary anti-rabbit HRP and anti-mouse HRP antibodies, were obtained from Sigma-Aldrich, whereas anti-rabbit Alexa555 and anti-mouse Cy-3 were obtained from Jackson laboratories (Bar Harbour, MA).

Sokołowska I., Piłka E.S., Sandvig K., Węgrzyn G, & Słomińska-Wojewódzka M. (2015). Hydrophobicity of protein determinants influences the recognition of substrates by EDEM1 and EDEM2 in human cells. BMC Cell Biology, 16, 1.

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