Sirnas against

Manufactured by Thermo Fisher Scientific

Sourced in Germany

SiRNAs (small interfering RNAs) are short, double-stranded RNA molecules that can be used to target and silence specific genes within cells. They function by binding to and degrading the messenger RNA (mRNA) of target genes, thereby preventing the production of the corresponding proteins.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Control sirna, by Thermo Fisher Scientific (2 mentions) Ab139690, by Abcam (1 mentions) Ab50818, by Abcam (1 mentions) Ab26109, by Abcam (1 mentions) Ab128874, by Abcam (1 mentions) General tissue culture materials, by Avantor (1 mentions) Gapdh mab374 antibody, by Merck Group (1 mentions) Anti rabbit igg a6667, by Merck Group (1 mentions) I bet151, by Bio-Techne (1 mentions) Marimastat, by Selleck Chemicals (1 mentions) Ly2886721, by Selleck Chemicals (1 mentions) Anti adam17, by Cell Signaling Technology (1 mentions) Anti bace2 antibody, by Santa Cruz Biotechnology (1 mentions) Anti adam9, by Cell Signaling Technology (1 mentions) On targetplus sirna, by Horizon Discovery (1 mentions) Negative sirna control, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Sirna, by Thermo Fisher Scientific (1 mentions) Ly294002, by Cell Signaling Technology (1 mentions)

8 protocols using sirnas against

Chromatin immunoprecipitation protocol for BRD proteins

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General tissue culture materials were obtained from VWR International. Antibodies against BRD2 (ab139690), BRD3 (ab50818), BRD4 (ab128874) and IRF1 (ab26109) antibody for chromatin immunoprecipitation (ChIP) were obtained from Abcam. Antibodies against c-MYC (5605), human PD-L1 (E1L3N clone; 13684), IRF1 (8478) and control IgG (2729) antibody for ChIP were purchased from Cell Signaling Technology. Anti-BRD4 (A301–985A) antibody for ChIP was obtained from Bethyl Laboratories. PE-conjugated human PD-L1 (MIH1 clone; 12-5983-42) antibody was from Thermo Fisher. The GAPDH (MAB374) antibody was from Millipore, and α-tubulin (sc-8035) antibody and HSP90 (sc-7940) antibody were obtained from Santa Cruz Biotechnology. Secondary anti-mouse IgG (A4416) and anti-rabbit IgG (A6667) antibodies were purchased from Sigma. The BET inhibitors JQ1 and I-BET151 were obtained from Tocris Bioscience, the BET PROTAC ARV-825 was obtained from MedChem Express, and human and mouse IFN-γ was purchased from Thermo Fisher Scientific. The siRNAs against BRD2, BRD3, BRD4, c-MYC, and IRF1 were purchased from Thermo Fisher Scientific.

Ebine K., Kumar K., Pham T.N., Shields M.A., Collier K.A., Shang M., DeCant B.T., Urrutia R., Hwang R.F., Grimaldo S., Principe D.R., Grippo P.J., Bentrem D.J, & Munshi H.G. (2018). Interplay between interferon regulatory factor 1 and BRD4 in the regulation of PD-L1 in pancreatic stellate cells. Scientific Reports, 8, 13225.

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Inhibition of ADAM Proteases in Cells

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Inhibitors LY2886721, Lanabecestat, and Marimastat were from Selleck Chemicals and TMI-1 was from Tocris Sciences. Anti-ADAM17 and anti-ADAM9 were from Cell Signaling Technology (Cat:3976S and 4151S, 1:000 dilution), anti-BACE2 antibody was from Santa Cruz Biotechnology (sc-271212, 1:1000 dilution), and anti-MSLN antibody (mouse monoclonal MN, 1 ug/ml) was from our lab. siRNAs against human ADAM17: s13719 (siADAM17_1) and si13720 (siADAM17_2) and one siRNA targeting ADAM10: s1004 were from Thermo Fisher Scientific. All other On-Target Plus siRNA and negative control siRNA luciferase GL2 were from Dharmacon.

Liu X., Chan A., Tai C.H., Andresson T, & Pastan I. (2020). Multiple proteases are involved in mesothelin shedding by cancer cells. Communications Biology, 3, 728.

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Targeting HIF1α and PFKFB3 in hiPSC-CMs

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siRNAs against HIF1α (siRNA ID: s6539, 4390824) and PFKFB3 (siRNA ID: s10359, 4390824), respectively, and a negative siRNA control (4390843) were purchased from Thermo Fisher (Waltham, MA). hiPSC-CMs were pre-treated with the above siRNAs for 72 h and incubated with human amylin as described previously.

Liu M., Li N., Qu C., Gao Y., Wu L, & Hu L.G. (2021). Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates. Communications Biology, 4, 188.

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Silencing PIM Kinases in HeLa Cells

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Small interfering RNAs (siRNAs) against human PIM-1, PIM-2 and PIM-3, and control siRNA were purchased from Invitrogen (Carlsbad, CA). MiR-1296 mimetic and miR-1299 mimetic were purchased from Sigma. The HeLa cells were transfected with siRNAs at 33 nM final concentration using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. For the miRNA transfection, HeLa cells were transfected with miR-1296 mimetic and miR-1299 mimetic at 100 nM final concentration using Lipofectamine RNAiMAX reagent (Invitrogen) according to the manufacturer's instructions. The transfected cells were used for subsequent experiments 24 h later.

Liu Z., He W., Gao J., Luo J., Huang X, & Gao C. (2015). Computational prediction and experimental validation of a novel synthesized pan-PIM inhibitor PI003 and its apoptosis-inducing mechanisms in cervical cancer. Oncotarget, 6(10), 8019-8035.

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Knockdown of AMPK and ZIPK in HeLa cells

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Small interfering RNAs (siRNAs) against human AMPK, ZIPK and control siRNA were purchased from Invitrogen (Carlsbad, CA). The HeLa cells were transfected with siRNAs at 100 nM final concentration using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. The transfected cells were used for subsequent experiments 24 h later.

Fu L., Zhang S., Zhang L., Tong X., Zhang J., Zhang Y., Ouyang L., Liu B, & Huang J. (2015). Systems biology network-based discovery of a small molecule activator BL-AD008 targeting AMPK/ZIPK and inducing apoptosis in cervical cancer. Oncotarget, 6(10), 8071-8088.

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Silencing Ribosomal Protein Genes

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SiRNAs against SPIN1, uL18 and uL5 were commercially purchased from Ambion. SiRNAs (20–40 nm) were introduced into cells using TurboFect transfection reagent following the manufacturer’s instruction. Cells were harvested 48–72 hr post-transfection for WB or RT-PCR.

Fang Z., Cao B., Liao J.M., Deng J., Plummer K.D., Liao P., Liu T., Zhang W., Zhang K., Li L., Margolin D., Zeng S.X., Xiong J, & Lu H. (2018). SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer. eLife, 7, e31275.

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Breast Cancer Cell Culture Protocols

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Human breast cancer cells SK-BR-3 and MDA-MB-231 were cultured in RPMI/10% FBS. BT-474 cells were cultured in RPMI/20% FBS/insulin. Human breast cancer cells MDA-MB-453 were cultured in Leibovitz/10% FBS under CO₂-free conditions. Mouse cell line mErbB2, generated from a primary MMTV-HER2 mouse tumor, were cultured on gelatin-coated plates in RPMI media with 15% FCS and PS, non-essential aminoacids, 1% pyruvate and EGF (0.02 μg/ml). Lapatinib for cell culture and CP724.714 was ordered by SelleckBiochem (Munich, Germany) and used as indicated. 17AAG (Calbiochem, Darmstadt, Germany), Ly294002 and U0126 (both Cell Signaling, Frankfurt, Germany) was used as indicated. siRNAs against HER2 (validated, IDs: s611 and s613), MIF (validated, IDs: s8780 and s194615) and HSF1 (pre-designed, IDs: s6950 and s6952) were purchased from Ambion (Hamburg, Germany) (Silencer select siRNAs). siRNAs were transfected with Lipofectamine2000 (Invitrogen, Darmstadt, Germany).

Schulz R., Streller F., Scheel A.H., Rüschoff J., Reinert M.C., Dobbelstein M., Marchenko N.D, & Moll U.M. (2014). HER2/ErbB2 activates HSF1 and thereby controls HSP90 clients including MIF in HER2-overexpressing breast cancer. Cell Death & Disease, 5(1), e980-.

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Manipulating miRNA and MMSET/Twist1 in EC Cells

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EC cells (50% confluence) were transfected with miRNA mimics and miRNA inhibitors for miR-34a, miR-424 and miR-513 (40 nM, Ambion, Austin, TX), siRNAs against MMSET or Twist1 (5 nM, Ambion, Austin, TX) and the expression vector for MMSET (OriGene, Rockville, MD) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. After Forty-eight hours, the cells were used for RNA extraction, protein extraction, transwell invasion assay and tumor sphere formation assay.

Dong P., Xiong Y., Yue J., Hanley S.J, & Watari H. (2018). miR-34a, miR-424 and miR-513 inhibit MMSET expression to repress endometrial cancer cell invasion and sphere formation. Oncotarget, 9(33), 23253-23263.

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