Pheatmap

Statistical analysis was performed using Prism 7 (Graphpad, La Jolla, CA) or RStudio (RStudio Team, 2015 ). For comparison between two groups, an unpaired T-test was used. For comparison between multiple groups, a one-way ANOVA was used with multiple comparisons to the control. Distributions were compared using a chi-squared test. Correlations were assessed using Pearson correlation co-efficient with two-tails. Graphing of the data was performed using Prism 7. Additional visualizations were created using ggplot2 (Wickham, 2009 ) and Pheatmap (Kolde, 2012 ) package for RStudio (RStudio Team, 2015 ). Vector diagrams were modified using Graphic (Autodesk). Statistics for differential gene expression from transcriptomic studies were performed at a dataset level with a False Discovery Rate (FDR) of 0.05 and represented in the graphs as such. An α level of 0.05 was used for all analyses. All values are means ± SEM unless noted otherwise. Asterisk (*), (**), (***) and (****) indicate experimental pairs where differences between the compared values were statistically significant (P < 0.05, P < 0.01, p < 0.001 and p < 0.0001, respectively).