S monovette 9 ml k3e

Manufactured by Sarstedt

Sourced in Germany

The S-Monovette® 9 mL K3E is a blood collection system designed for the collection of 9 mL of venous blood. It features a closed system and a pre-evacuated tube to facilitate blood collection.

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Lab products found in correlation

Macs system, by Miltenyi Biotec (2 mentions) Allprep dna rna mini kit, by Qiagen (2 mentions) S monovette, by Sarstedt (1 mentions) S monovette 5 ml 9nc, by Sarstedt (1 mentions) Rnalater stabilization solution, by Thermo Fisher Scientific (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Ez dna methylation gold kit, by Zymo Research (1 mentions)

Spelling variants of this product

S-Monovette (283 citations) Monovettes (21 citations) S-Monovette 9 mL (7 citations) S-Monovette K3E (4 citations) S-Monovette 4.9 mL K3E (2 citations) S-Monovette® EDTA K3E/4.9 ml (2 citations)

9 protocols using s monovette 9 ml k3e

Plasma Generation and Matrices Comparison

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Whole blood was centrifuged for 10 min at 3000 rcf (3000 g) for plasma generation. The matrix comparison experiment included four matrices, serum, heparin plasma, EDTA plasma and citrate plasma (S-Monovette^® 5.5 mL Z, S-Monovette^® 5.5 mL LH, S-Monovette^® 9 mL K3E, S-Monovette^® 5 mL 9NC; all: SARSTEDT AG&Co.KG, Nürmbrecht, Germany), whereas all experiments were conducted in heparin plasma. Plasma samples were aliquoted and stored at −80 °C until analysis.
The majority of experiments were conducted in anonymized residual research samples. Samples for the matrix comparison experiment (maximum 25 mL) were collected as part of quality control for biobanked blood samples. Blood samples were taken from patients of the Department of Cardiology during routine venipuncture or from healthy individuals after informed consent for blood collection for the Cardiovascular Biobank of the German Heart Centre Munich was obtained. The blood collection for biobanking was approved by the Ethics Commission of the Technical University Munich (Nr. 5943/13; 16 October 2013).

Krueger K., Mayer Z., Gerckens M., Boeck S., Luppa P, & Holdenrieder S. (2022). High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in Blood. Biomedicines, 10(10), 2405.

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Plasma Collection and Esophageal Biopsy Protocol

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From each subject, 9 mL of peripheral blood was collected into a tube containing 0.5 M EDTA (S-Monovette® 9 mL K3E, Sarstedt, Germany). Plasma was separated from these samples by centrifugation (2000 g, 4°C, 10 min) within 60 minutes of collection, aliquoted (6 × 300 μL), and stored at −70°C until ELISA analysis. The remaining plasma was used for DNA isolation from leukocytes based on the modified salting-out method with proteinase K digestion of cells [31 (link)].
The biopsies from 23 patients with RE, BE, or EAC were collected only at the Department of Gastroenterology, University Hospital Brno, Czech Republic. Four biopsies were taken from each patient's esophagus during the endoscopic examination of the upper GIT. Two samples were collected from the part with endoscopically visible pathological changes and two from the part without such apparent changes. In this way, we acquired two pairs of samples, each pair containing one sample from the seemingly pathological and one from the seemingly healthy tissue. One pair was placed into 1.8 mL cryovials (SPL Life Sciences, Korea) with 1 mL of RNAlater™ Stabilization Solution (Thermo Fisher Scientific, Waltham, MA, USA) and stored at −70°C until RNA extraction. The other pair was sent to the Department of Pathology, Faculty Hospital Brno, Czech Republic, for histopathological confirmation of the diagnosis.

Deissova T., Cvanova M., Kala Z., Jiraskova Zakostelska Z., Dolina J., Kunovsky L., Kroupa R., Pavlovsky Z., Lipovy B., Danek Z., Izakovicova Holla L., Urban O., Navratil V., Lischke R., Harustiak T., Grolich T., Prochazka V., Slaby O, & Borilova Linhartova P. (2022). Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett's Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study. Disease Markers, 2022, 8790748.

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Plasma Isolation and HLA Typing Protocol

Cited 1 time

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Cells were lysed as previously described [30 (link)]. Plasma from donors was generated by drawing blood with EDTA tubes (S-Monovette 9 ml K3E, Sarstedt). Tubes were readily inverted, incubated for 30 min at RT and centrifuged at 2’000g for 10 min. Plasma was transferred into new vials, snap-frozen and stored at -80°C. After thawing, plasma was cleared by centrifugation at 3’345g for 5 min, 4°C prior to HLA purification. All donors were from the Department of Nephrology (Tenon Hospital Paris, France) and provided written informed consent for usage of plasma and clinical data for scientific purposes. Donors one and two were healthy volunteers, donors three and four were patients suffering from the rare kidney disease membranous nephropathy. This project was approved by the IRB/Ethics committee CPP Ile-de-France IV, Hôpital Saint-Louis, Paris (ethic vote 2013/03/14).
Volunteers were HLA typed by NGS technologies resulting in an unambiguous annotation of the A, B, C, DR and DQ alleles [Supporting Information Table 1].

Ritz D., Sani E., Debiec H., Ronco P., Neri D, & Fugmann T. (2018). Membranal and blood-soluble HLA class II peptidome analyses using data-dependent and independent acquisition. Proteomics, 18(12), e1700246.

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Quantification of IL-8 in Human Plasma

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Levels of IL-8 in plasma were measured in all 153 individuals at the Department of Biochemistry, Faculty of Medicine, Masaryk University, Brno. The plasma samples were prepared from venous blood collected into a tube with EDTA (S-Monovette^® 9 mL K3E, Sarstedt, Germany), separated by centrifugation (465× g, 4 °C, 10 min), and stored at −70 °C within 30 min of collection.
IL-8 plasma levels were determined using enzyme-linked immunosorbent assay (ELISA) kits [IL-8 Human Magnetic Kit for Luminex™ Platform (Catalog No. LHC0081M, Novex™, Life Technologies, Grand Island, NY, USA) with Human/Monkey Extracellular Protein Buffer Magnetic Reagent Kit (Catalog No. LHB0001M, Novex™, Life Technologies, Grand Island, NY, USA)] and software (Luminex 200^TM analyzer with xPONENT 3.1 Software, Luminex Corporation, USA; Milliplex^TM Analyst v 3.4 Software, VigeneTech, Carlisle, MA USA) according to the manufacturer’s instructions. Those samples with IL-8 values under the limit of detection (<4.40 pg/mL) were arbitrarily assigned a value of 4.39 pg/mL for the statistical analysis.

Borilova Linhartova P., Kavrikova D., Tomandlova M., Poskerova H., Rehka V., Dušek L, & Izakovicova Holla L. (2018). Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status. International Journal of Molecular Sciences, 19(10), 3214.

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Genetic Variation Profiling of Monocytes

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9 ml blood was drawn in the morning between 7 and 9 am (S-Monovette 9 ml K3E, Sarstedt). Monocytes were immunomagnetically purified from whole blood with the MACS System (Miltenyi Biotec), shock frozen and stored at − 80 °C. Cell homogeneity was determined with the BD FACSCanto TM II Flow Cytometer (BD Biosciences), and showed high purity (98.3 ± 2.4% (SD). DNA was isolated with the AllPrep RNA/DNA Mini Kit (Qiagen, 80204).
5-HTTLPR polymorphism was analyzed as previously described^{46 (link)}. FKBP5 genotype of rs1360780 was evaluated using high resolution melt analysis (HRM; Biorad). Further information can be found in Supplementary Table S10.

Hummel E., Elgizouli M., Sicorello M., Leitão E., Beygo J., Schröder C., Zeschnigk M., Müller S., Herpertz S., Moser D., Kessler H., Horsthemke B, & Kumsta R. (2022). No evidence for intervention-associated DNA methylation changes in monocytes of patients with posttraumatic stress disorder. Scientific Reports, 12, 17347.

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EDTA Plasma Collection and Storage

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Blood samples were collected in EDTA Monovettes (S-Monovette 9 ml K3E; Sarstedt, Nümbrecht, Germany) and centrifuged for 10 min at 2000 × g at room temperature to obtain EDTA blood plasma. Until use, EDTA plasma was stored in 500 μl aliquots in polypropylene tubes at − 80 °C.

Shahpasand-Kroner H., Klafki H.W., Bauer C., Schuchhardt J., Hüttenrauch M., Stazi M., Bouter C., Wirths O., Vogelgsang J, & Wiltfang J. (2018). A two-step immunoassay for the simultaneous assessment of Aβ38, Aβ40 and Aβ42 in human blood plasma supports the Aβ42/Aβ40 ratio as a promising biomarker candidate of Alzheimer’s disease. Alzheimer's Research & Therapy, 10, 121.

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Plasma Biomarkers for Alzheimer's Disease

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Plasma samples of Control and AD cases were obtained from the biobank of the Universitätsmedizin Göttingen. The pseudonymized collection of biological samples and clinical data and their use in biomarker studies were approved by the ethics committee of the University Medical Center Goettingen (approval 9/2/16). All subjects or their legal representatives gave their informed consent prior to inclusion. Biomaterial sampling and data collection were conducted according to the revised Declaration of Helsinki and good clinical practice guidelines.
Plasma was collected in EDTA Monovettes (S-Monovette 9 mL K3E; Sarstedt, Nümbrecht, Germany), centrifuged at 2000× g for 10 min, at room temperature (RT), to obtain EDTA blood plasma. Samples were aliquoted and stored at −80 °C. The Controls group included 15 age-matched individuals with a mean age of 65.60 ± 9.36 years, and the AD group comprised 15 individuals with a mean age of 67.00 ± 9.82 years (p-value = 0.69). AD diagnosis followed the 2011 McKhann criteria and included cognitive testing (Mini-Mental State Examination and Clock-Drawing Test), CSF neurochemical biomarker triplet assessment, and/or PET scan imaging.

Soares Martins T., Pelech S., Ferreira M., Pinho B., Leandro K., de Almeida L.P., Breitling B., Hansen N., Esselmann H., Wiltfang J., da Cruz e Silva O.A, & Henriques A.G. (2024). Phosphoproteome Microarray Analysis of Extracellular Particles as a Tool to Explore Novel Biomarker Candidates for Alzheimer’s Disease. International Journal of Molecular Sciences, 25(3), 1584.

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Immunomagnetic Monocyte Isolation and DNA Methylation

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Nine ml blood was drawn in the morning between 7-9 am (S-Monovette 9 ml K3E, Sarstedt). Monocytes were immunomagnetically purified from whole blood with the MACS System (Miltenyi Biotec, Bergisch Gladbach, Germany), shock frozen and stored at -80°C. DNA was isolated with the AllPrep RNA/DNA Mini Kit (Qiagen, Hilden, Germany). For DBS, DNA was bisulfite-modified with the EZ DNA Methylation Gold Kit (Zymo Research, Orange, CA, USA) and processed as described elsewhere [42, 43] using primers as indicated in S2 Table.

Hummel E., Elgizouli M., Beygo J., Giuranna J., Sicorello M., Leitão E., Schröder C., Zeschnigk M., Müller S., Öztürk D., Föcker M., Herpertz S., Hebebrand J., Moser D., Kessler H., Horsthemke B., Hinney A., & Kumsta R. (2020). No evidence for intervention-associated DNA methylation changes in monocytes of patients with posttraumatic stress disorder or anorexia nervosa.

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Postpartum Liver and Blood Sampling

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Blood samples were collected on d 7 p.p. between 0700 and 0800 h (after milking and before feeding) from tail vein into EDTA-containing vacutainers (S-Monovette 9 mL K3E, Sarstedt, Nümbrecht, Germany) and kept on ice for <1 h. Plasma was obtained by centrifugation at 2,000 × g for 10 min at 4°C and stored at -20°C. Biopsies of the liver were also taken on d 7 p.p. at 0900 h between the 9th and 10th ribs approximately 15 cm below the transverse processes by means of a self-made barbed 12-gauge × 27 cm biopsy needle under local anesthesia, as described in detail (Zeitz et al., 2018) (link). Liver samples were immediately snap-frozen in liquid N 2 and stored at -80°C.

Ringseis R., Zeitz J.O., Weber A., Koch C, & Eder K. (2019). Hepatic transcript profiling in early-lactation dairy cows fed rumen-protected niacin during the transition from late pregnancy to lactation. Journal of dairy science, 102(1).

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