2 4 6 tris 2 pyridyl s triazine tptz

Alpha amylase from porcine pancreas (U/mg), mucin, (m2378), Pepsin from porcine gastric mucosa (U/mg), Pancreatin from porcine pancreas (U/mg), Adenosine di phosphate (ADP), 1, 1, 3, 3- Tetramethoxypropan Ascorbic acid, FeSO₄, NaCl, Disodiumorthophosphate (Na₂HPO₄), Pottasium dihydrogenphosphate (KH₂PO₄), HCl, TrichloroAcetic acid (TCA), Thiobarbituric acid (TBA), 2,4-Dinitrophenylhydrazine (DNPH) phosphoric acid and Acetic acid were purchased from Sigma-Aldrich Company. Reagents used for HPLC analysis were HPLC grade and purchased from VWR International (Lutterworth, UK). FRAP reagents included sodium acetate, glacial Acetic acid, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), HCl (Fisher Scientific UK Ltd), FeCl₃·6H₂O and FeSO₄·7H₂O. HORAC reagents included gallic acid, fluorescein sodium salt, H₂O₂, cobalt (II) fluoride and picolinic acid. All chemicals for the FRAP and HORAC assays were obtained from Sigma-Aldrich Company unless otherwise stated.

A Folin–Ciocalteu’s (FC) reagent, a total phenolic content (TPC) standard, iron (II) chloride hexahydrate (98%), iron (III) chloride hexahydrate (97%), iron (II) sulphate heptahydrate (99%), hydrogen chloride (HCl, 99%), sodium acetate, 2,4,6-tris-(2-pyridyl)-s-triazine (TPTZ > 98%), sinapic acid (>97%), 2,2-diphenyl-1-picrylhydrazyl (DPPH, 97%), and formic acid were all purchased from Fisher Scientific Canada Ltd. (Ottawa, ON, Canada). Aluminum chloride (AlCl₃), sodium nitrite (NaNO₂), sodium carbonate (Na₂CO₃), sodium hydroxide (NaOH), ferrozine, and disodium ethylenediaminetetraacetic acid (Na₂EDTA) were purchased from Sigma Canada Ltd. (Mississauga, ON, Canada). Quercetin hydrate (>95%) and 2-amino-ethyl-diphenyl borate (98%) were purchased from Acros (Mississauga, ON, Canada).
Extraction reagents, including methanol (optima grade) and ethanol (analytical grade), and standard compounds for high-performance liquid chromatography (HPLC) were purchased from Sigma Canada Ltd. (Mississauga, ON, Canada).

Folin–Ciocalteu’s phenol reagent, ferric chloride hexahydrate (FeCl₃·6H₂O), ferrous sulfate heptahydrate (FeSO₄·7H₂O), and hydrogen chloride (hydrochloric acid ≥ 37% w/w) were purchased from Chem-Lab NV (Zedelgem, Belgium). Sodium carbonate was obtained from Carlo Erba Reagents (Cornaredo, Italy), and absolute ethanol and acetic acid from Merck KgaA (Darmstadt, Germany). Gallic acid (3,4,5-trihydroxybenzoic acid), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid ammonium salt) (ABTS non radical form) and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were bought from Alfa Aesar (Karlsruhe, Germany), while Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) and potassium persulfate were supplied from Sigma-Aldrich (St. Louis, MO, USA). Standard phenolic compound of chlorogenic acid (≥99%) was purchased from Extrasynthese (Genay, France), and naringenin (97%) and 4-chloro-4’-hydroxybenzophenone (97%) were from Alfa Aesar (Karlsruhe, Germany). Formic acid was bought from LGC Standards (Wesel, Germany). All reagents used for the spectrophotometric assays were of analytical grade (≥94%). Solvents used for all experimental analyses were of LC-MS grade and were purchased from Sigma Aldrich Co. (Gillingham, United Kingdom), Carlo Erba Reagents (Val de Reuil, France), Chem-Lab NV (Zedelgem, Belgium), Fischer-Scientific (Loughborough, UK), and Merck KGaA (Darmstadt, Germany).