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Cd122 pe dazzle 594 clone beta1

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CD122-PE-Dazzle 594 (clone TM-beta1) is a fluorochrome-conjugated antibody that binds to the CD122 antigen. CD122 is the beta chain of the interleukin-2 receptor (IL-2R) complex. This antibody can be used to identify and study cells expressing CD122, such as natural killer (NK) cells and regulatory T cells.

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Lab products found in correlation

Lsrfortessa x 20, by BD (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Anti cd25 pe cy7 clone pc61.5, by Thermo Fisher Scientific (2 mentions) Anti pd 1 bv785 clone 29f 1a12, by BioLegend (2 mentions) Foxp3 transcription factor staining buffer set protocol, by Thermo Fisher Scientific (2 mentions) Anti cd3zeta fitc clone h146 968, by Abcam (2 mentions) Anti foxp3 efluor660 clone 150d e4, by Thermo Fisher Scientific (2 mentions)

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CD122 (3 citations)

2 protocols using cd122 pe dazzle 594 clone beta1

1

Comprehensive Splenic T-cell Immunophenotyping

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Splenic single cell suspensions were washed with PBS containing 2% FCS and labeled for 30 minutes at 4°C for a combination of cell surface markers with the following fluorescently labeled anti-mouse antibodies: anti-CD4-BUV395 (clone GK1.5), anti-CD44-V450 (clone IM7) (BD Horizon, Franklin Lakes, NJ, USA); anti-CD25-PE-Cy7 (clone PC61.5) (eBioscience); CD122-PE-Dazzle 594 (clone TM-beta1), and anti-PD-1-BV785 (clone 29F.1A12) (BioLegend, San Diego, CA, United States). Live/Dead™ Fixable Aqua Dead Cell Stain Kit (Invitrogen) was included in the cell surface labeling to assess cell viability. Cells were subsequently labeled intracellularly according to the FoxP3 Transcription Factor staining buffer set protocol (eBioscience) with anti-CD3zeta-FITC (clone H146–968) (Abcam, Cambridge, Cambridgeshire, United Kingdom) and anti-FoxP3-eFluor660 (clone 150D/E4) (eBioscience). Labeled cells were detected on a BD LSRFortessa X-20 (BD Biosciences, Franklin Lakes, NJ, United States). Data analysis was performed using FlowJo software (Tree Star, Ashland, OR, United States).
Pieren D.K., Smits N.A., Imholz S., Nagarajah B., van Oostrom C.T., Brandt R.M., Vermeij W.P., Dollé M.E, & Guichelaar T. (2021). Compromised DNA Repair Promotes the Accumulation of Regulatory T Cells With an Aging-Related Phenotype and Responsiveness. Frontiers in Aging, 2, 667193.
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2

Multiparametric Flow Cytometry of Splenic Immune Cells

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Splenic single cell suspensions were washed with PBS containing 2% FCS and labeled for 30 minutes at 4°C for a combination of cell surface markers with the following fluorescently labeled anti-mouse antibodies: anti-CD4-BUV395 (clone GK1.5), anti-CD44-V450 (clone IM7) (BD Horizon, Franklin Lakes, NJ, USA); anti-CD25-PE-Cy7 (clone PC61.5) (eBioscience); CD122-PE-Dazzle 594 (clone TM-beta1), and anti-PD-1-BV785 (clone 29F.1A12) (BioLegend, San Diego, CA, United States). Live/Dead™ Fixable Aqua Dead Cell Stain Kit (Invitrogen) was included in the cell surface labeling to assess cell viability. Cells were subsequently labeled intracellularly according to the FoxP3 Transcription Factor staining buffer set protocol (eBioscience) with anti-CD3zeta-FITC (clone H146–968) (Abcam, Cambridge, Cambridgeshire, United Kingdom) and anti-FoxP3-eFluor660 (clone 150D/E4) (eBioscience). Labeled cells were detected on a BD LSRFortessa X-20 (BD Biosciences, Franklin Lakes, NJ, United States). Data analysis was performed using FlowJo software (Tree Star, Ashland, OR, United States).
Pieren D.K., Smits N.A., Imholz S., Nagarajah B., van Oostrom C.T., Brandt R.M., Vermeij W.P., Dollé M.E, & Guichelaar T. (2021). Compromised DNA Repair Promotes the Accumulation of Regulatory T Cells With an Aging-Related Phenotype and Responsiveness. Frontiers in Aging, 2, 667193.
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