For systemic administration studies, mice received daily intraperitoneal injections of 15 mg/kg CsA (Sandimmune® oral injection; Novartis, East Hanover, NJ) or olive oil vehicle (Sigma, St. Louis, MO) for 15 days before behavioral testing. Drug administration continued during behavioral testing. All drugs were injected in a volume of 10 ml/kg body weight.
Sandimmune
Manufactured by Novartis
Sourced in Switzerland, United States
Sandimmune is an immunosuppressant medication used to prevent organ rejection in transplant patients. It is a laboratory product that functions to inhibit the activation and proliferation of T-cells, which play a crucial role in the body's immune response.
Automatically generated - may contain errors
Lab products found in correlation
Neoral, by Novartis (4 mentions)
Prograf, by Astellas Pharma (3 mentions)
Cyclosporine a, by Novartis (3 mentions)
Thymoglobulin, by Sanofi (2 mentions)
Cellcept, by Roche (2 mentions)
Simulect, by Novartis (1 mentions)
Quattro premier mass spectrometer, by Waters Corporation (1 mentions)
Ketoconazole, by Meilun (1 mentions)
Cremophor el, by Novartis (1 mentions)
Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Dnase, by Thermo Fisher Scientific (1 mentions)
Detachabead cd19, by Thermo Fisher Scientific (1 mentions)
Anti cd19 dynabeads, by Thermo Fisher Scientific (1 mentions)
Cyclosporine, by Novartis (1 mentions)
Gentlemacs, by Miltenyi Biotec (1 mentions)
Sucrose, by Thermo Fisher Scientific (1 mentions)
Sliding microtome, by Leica (1 mentions)
Rapamune, by Pfizer (1 mentions)
Nulojix, by Bristol-Myers Squibb (1 mentions)
31 protocols using sandimmune
1
Cyclosporine A administration protocol
2
Immunosuppressant Regimen for Kidney Transplant
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We administered basiliximab (Simulect, 20 mg on days 0 and 4; Novartis, Basel, Switzerland) for KTRs with low immunologic risk and antithymocyte globulin (Thymoglobulin, 1.5 mg/kg on day 0 and 1.0 mg/kg from day 1 to day 3; Genzyme, Cambridge, MA, USA) for KTRs with high immunologic risk as induction immunosuppressants. We administered cyclosporine (Sandimmune, 3 mg/kg, twice a day; Novartis AG, Basel, Switzerland) or tacrolimus (Prograf, 0.05 mg/kg, twice a day; Astellas Pharma Inc., Toyama, Japan) as a CNI, prednisolone (30 – 20 – 10 – 5 – 0 mg, once a day, on a stepdown regimen), and mycophenolate mofetil (CellCept, 750 or 1,000 mg, twice a day for 1 month after KT and subsequent 500 mg, twice a day; Hoffmann-La Roche Inc., Nutley, NJ, USA) as maintenance immunosuppressants. The treatment protocol for recurrent IgAN was use of an angiotensin receptor blocker at diagnosis; if it elicited no response, then 0.25 mg/kg per day of oral steroid was used.
3
Immunosuppressant-Loaded Glaucoma Implants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PLA-PEG glaucoma implants were preliminary saturated with immunosuppressants. Saturation with CsA was performed ex tempore in operation room just before implantation: samples were placed in 1:30 dilution of CsA concentrate (50 μg/mL, Sandimmune, Novartis Pharna, Basel, Switzerland) and BSS for 15 min. Before implantation it was dried with sterile blotting paper. Saturation of implants with everolimus was performed before sterilization by means of ultrasonic exposure (power 630 Wt, frequency 22 kHz) in everolimus suspension 2% for 6 min.
4
Transplantation of hESC-RPE Cells in RCS Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were conducted according to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and approved by the Institutional Committee for Animal Research of the Hebrew University-Hadassah Medical School.
GFP+ cryopreserved hESC-RPE cells were thawed and transplanted into RCS rats as described in Idelson et al. (2009) (link). One day prior to the surgery, animals allocated for cyclosporine therapy were started on a maintenance dose of oral cyclosporine A (210 mg/L; Sandimmune, Novartis Pharma AG, Basel, Switzerland) in the drinking water until sacrificing. In vivo imaging, ERG recordings, and histological and immunohistochemical examinations were done as described previously (Idelson et al., 2009 (link)). Mouse anti-GFP FITC-labeled antibody (1:100, no. sc-9996 FITC, Santa Cruz Biotechnology, Santa Cruz, CA) was used for immunohistochemical analysis. Serum samples were analyzed for IL-10 concentration by using ELISA kit (DRG Instruments, Marburg, Germany).
GFP+ cryopreserved hESC-RPE cells were thawed and transplanted into RCS rats as described in Idelson et al. (2009) (link). One day prior to the surgery, animals allocated for cyclosporine therapy were started on a maintenance dose of oral cyclosporine A (210 mg/L; Sandimmune, Novartis Pharma AG, Basel, Switzerland) in the drinking water until sacrificing. In vivo imaging, ERG recordings, and histological and immunohistochemical examinations were done as described previously (Idelson et al., 2009 (link)). Mouse anti-GFP FITC-labeled antibody (1:100, no. sc-9996 FITC, Santa Cruz Biotechnology, Santa Cruz, CA) was used for immunohistochemical analysis. Serum samples were analyzed for IL-10 concentration by using ELISA kit (DRG Instruments, Marburg, Germany).
5
Investigating Immunosuppressant Pharmacokinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rapamycin (Rapamune, Wyeth Australia) was diluted in PBS and administered (0.6 mg/kg) by i.p. injection. Cyclosporine (Sandimmune, Novartis Pharmaceuticals Australia) was diluted in PBS and administered (25 mg/kg) daily by i.p. injection. Immunosuppressant administration commenced on the day of BM/HSPC transfer and continued daily for the following 21 days unless the experiment finished sooner. To determine the blood concentration of rapamycin, whole blood was collected in 0.5 M EDTA immediately prior to rapamycin administration on the days indicated and stored at −30 °C. LC-MS/MS analysis was performed using an Alliance HT LC system interfaced to a Quattro-Premier mass spectrometer (Waters Corporation, Milford, MA, USA).
6
Cyclosporine and Zamzam Water Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cyclosporine (Sandimmune®, 50 mg/mL ampule) was purchased from Novartis (Istanbul, Turkey). ZW was brought from the Zamzam well located within the holy mosque in Makkah, Saudi Arabia.
7
CsA Formulation for In Vitro and In Vivo Use
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For in vitro experiments, CsA (Sigma) was dissolved at a con centration of 50 mM in EtOH and stored at -20 °C. For in vivo treatment, CsA (Sandimmune ® , Novartis) was formulated in PBS and administered to mice as an i.p. bolus (100 μl) dose of 5 mg/kg.
8
Spinal Cord Injury Transplantation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experimental design is shown in Figure 1 A,B. Adult (225–250 g) female Sprague Dawley rats received a dorsal column lesion at C4 followed by a chronic transplantation of NPC or medium injection. All animals were given daily subcutaneous injections of cyclosporine A (10 mg/kg; Sandimmune; Novartis Pharmaceuticals, East Hanover, NJ, USA) that began 3 days before transplantation or medium injection. Buprenorphine (Webster Veterinary, Sterling, MA, USA) was used for pain relief every 12 h for 3 days and then as needed. Animals were divided into the following groups (Figure 1 C): control: 4 weeks delay (n = 11), cNPCs (cultured NPCs): 4 weeks delay (n = 3); dNPCs (dissociated NPCs): 4 weeks delay (n = 10), 6 weeks delay (n = 5), 12 weeks delay (n = 5). All procedures were carried out in accordance with protocols approved by the Institutional Animal Care and Use Committee (IACUC) of Drexel University, following the NIH Guide for the care and use of laboratory animals.
9
Evaluating Novel Chemomodulators Using Q3GA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Q3GA was purchased from Chengdu Sino Standards Bio-Tech Co., Ltd. (Chengdu, China). CsA, CsD, verapamil (VER) and ketoconazole (KET) were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). The CsA preparation was Sandimmune® injection (50 mg/ml; Novartis International AG, Basel, Switzerland) including Cremophor® EL (polyethoxylated castor oil), and the infusion concentrate was diluted in normal saline prior to use.
10
Cyclosporine Infusion Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!