Migration and invasion assays were performed in 24-well millicell hanging insert (Millipore) or 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences). In brief, 1 × 105 cells were seeded to the top chamber and 10% FBS in medium was added to the bottom chamber as a chemoattractant. After 24 or 48-h incubation, the number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 fields and imaged using SPOT imaging software (Nikon).
24 well biocoat matrigel invasion chambers
Manufactured by BD
Sourced in United States
The 24-well BioCoat Matrigel Invasion Chambers are a laboratory equipment designed to assess the invasive potential of cells. The product consists of cell culture inserts with a Matrigel-coated membrane, allowing for the study of cell migration and invasion through a reconstituted basement membrane.
Automatically generated - may contain errors
Lab products found in correlation
Spot imaging software, by Nikon (5 mentions)
24 well millicell hanging insert, by Merck Group (2 mentions)
24 well milli cell hanging insert, by BD (2 mentions)
24 well migration chamber, by Corning (1 mentions)
Bz x700 microscope system, by Keyence (1 mentions)
Diff quik staining kit, by Sysmex (1 mentions)
11 protocols using 24 well biocoat matrigel invasion chambers
1
Cell Migration and Invasion Assay
2
Cell Migration and Invasion Assay
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Cell ability of migration and invasion were performed using 24-well migration chamber (Corning, NY) or 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences, CA) with polyethylene terephthalate (PET)-based membrane of 8.0-um pore size. In Brief, 1x105 cells were suspended with serum-free culture medium and seeded in the top chambers. Complete culture medium with 10% FBS was used in the bottom chambers. The chambers were collected after 24/48 hours’ incubation. Cells which moved through the membrane were fixed with 4% PFA and stained with 0.5% crystal violet solution. The number of cells invaded through the membrane per field were counted and imaged using SPOT imaging software (Nikon) under microscope.
3
Cell Migration and Invasion Assay
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Migration and invasion assays were performed in 24-well millicell hanging insert (Millipore) or 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences). In brief, 1×105 cells were seeded to the top chamber and 10% FBS in medium was added to the bottom chamber as a chemoattractant. After 24 h or 48 h incubation, the number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 fields and imaged using SPOT imaging software (Nikon).
4
Comprehensive Cell Migration and Invasion Assays
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Cell migration was assessed by wound-healing and Transwell assays. For the wound-healing assay, a scratch was made in the center of the culture dish using a sterile 20-µL pipette tip before cells were fully confluent. The wound was observed using a bright-field microscope at 24- and 48-h after scratching. The migration rate = (scratch widths before migration – scratch widths after migration)/scratch widths before migration ×100%. Transwell migration assay was conducted using 24-well Transwell Chambers without Matrigel (BD Biosciences) according to the manufacturer’s instructions. Briefly, 1×105 Huh7 cells or 1×105 CLC13 cells in medium with 10% FBS were added to the top chamber, and the medium containing 20% FBS was added to the bottom chamber as an attractant. After 48-h incubation, the migrated cells were fixed and stained with crystal violet. The number of migrated cells was counted in 10 fields under a 10× objective lens.
Transwell invasion assay was conducted using 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences) according to the manufacturer’s instructions. Briefly, 4×105 Huh7 cells in medium with 10% FBS were added to the top chamber, and the medium containing 20% FBS was added to the bottom chamber as an attractant. The remaining steps are the same as those described for the Transwell migration assay.
Transwell invasion assay was conducted using 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences) according to the manufacturer’s instructions. Briefly, 4×105 Huh7 cells in medium with 10% FBS were added to the top chamber, and the medium containing 20% FBS was added to the bottom chamber as an attractant. The remaining steps are the same as those described for the Transwell migration assay.
5
Cell Migration and Invasion Assay
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Migration and invasion assays were performed in 24-well milli-cell hanging insert (BD Biosciences) or 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences). In brief, 5 × 104 cells were seeded to the top chamber and 10% FBS in medium was added to the bottom chamber as a chemoattractant. After 24 or 48 h incubation, the number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 fields and imaged using SPOT imaging software (Nikon).
6
Matrigel Invasion Assay for Cancer Cell Migration
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We used 24‐well BioCoat Matrigel Invasion Chambers (BD Biosciences, Franklin, New Jersey, USA) to evaluate migratory and invasive abilities of GC cells. 3 × 104/well cells were suspended in 200 μL serum‐free medium and were seeded into the upper chamber containing an uncoated or matrigel‐coated membrane, and then complete medium (700 μL) was added into lower chamber. 0.1% crystal violet was used to stain the cells that invaded or migrated to the low membrane after 24‐hour incubation at 37°C in a humidified 5% CO2 incubator. The experiments were performed in triplicates.
7
Invasion Assay of Hep3B Cells
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The invasion ability of Hep3B cells was assessed in 24‐well Biocoat Matrigel invasion chambers with an 8‐µm pore size (BD Biosciences) according to the manufacturer’s recommended protocol. Briefly, 48 hours following transfection with MYCN siRNAs or miR‐493‐5p mimics and MYCN expression vector, 50 000 cells were plated in the upper chamber with serum‐free medium. The bottom chamber contained 20% FBS as a chemoattractant. After incubation for 72 hours, cells that did not migrate through the membrane were removed using a cotton swab. Invasive cells on the lower surface of the membrane were fixed with methanol and stained using a Diff‐Quik staining kit (Sysmex). Cells were automatically counted using a BZ‐X700 microscope system (Keyence), and the average number of cells per field was calculated.
8
Cell Migration and Invasion Assays
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Migration and invasion assays were performed in 24-well milli-cell hanging insert (BD Biosciences) or 24well BioCoat Matrigel Invasion Chambers (BD Biosciences). In brief, 5×10 4 cells were seeded to the top chamber and 10% FBS in medium was added to the bottom chamber as a chemoattractant. After 24 or 48h incubation, the number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 fields and imaged using SPOT imaging software (Nikon).
9
Matrigel Invasion Assay Protocol
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Invasion assay was carried out according to the manufacturer’s instructions (BD BioCoat 24-well Matrigel invasion chambers). Invasion of treated cells was expressed as a percentage relative to the control cells.
10
GTP Rescue of Matrigel Invasion
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Invasion assay was carried our according to manufacturer’s instructions (BD BioCoat 24 well matrigel invasion chambers). GTP (1mM, Sigma) was added both in upper and lower chambers for invasion rescue. Invasion of mtp53 knockdown cells was expressed as a percentage relative to control cells.
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