β2m mice, by Jackson ImmunoResearch - Product details

1

Generating Genetically Modified Mouse Models

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K^bD^b−/− mice were purchased from Taconic Biosciences and then maintained in-house. B6.SJL mice (Taconic) were crossed with K^bD^b−/− mice to obtain congenic K^bD^b−/− (K^bD^b−/−.SJL) animals. For certain experiments, K^bD^b−/− and K^bD^b−/−.SJL mice were used interchangeably as K^bD^b−/−. CD1d^−/− mice (Jackson Laboratory) and RAG1^−/− mice (Jackson Laboratory) were crossed with K^bD^b−/− mice to obtain CD1d^−/−K^bD^b−/− and RAG1^−/−K^bD^b−/− animals, respectively. C57BL/6 and β₂m^−/− mice were obtained from Jackson Laboratory. C57BL^/6 mice were used as wild-type controls. None of the experiments within these studies were blinded or randomized. Both age- and sex-matched mice were used for this study, which was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals, as defined by the National Institutes of Health (PHS Assurance #A3284–01). Animal protocols were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of Brown University. All animals were housed in a centralized and AAALAC-accredited research animal facility that is fully staffed with trained husbandry, technical, and veterinary personnel.

Anderson C.K., Reilly E.C., Lee A.Y, & Brossay L. (2019). Qa-1-Restricted CD8+ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection. Cell reports, 27(2), 537-548.e5.

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2

Behavioral analysis of β2m-/- mice

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β2m^−/− mice (maintained on a C57BL/6J background) were purchased from a commercial supplier (Jackson Labs, Bar Harbor, ME, USA). WT and β2m^−/− mice were generated by heterozygous mutants, and we used WT littermates or WT C57Bl/6J mice (Nippon SLC, Inc., Shizuoka, Japan) as a control group for the β2m^−/− mice. All the mice were housed in specific pathogen-free (SPF) facility and behavioral analysis was also done there. In-house monitoring was performed every 3 months using a Monilisa IVA kit (Wakamoto Pharmaceutical Co., Ltd., Tokyo, Japan) that detects four major organisms, Sendai virus, mouse hepatitis virus, mycoplasma and Tyzzer’s organism [25] (link). No infections were detected in any of the rooms in which mice used in this study were maintained. Male mice were used in the present study. Mice were housed in groups of three to six animals until 2 weeks before the first experiment and then individually in standard laboratory Plexiglas cages under a 12-h light/12-h dark schedule (lights on at 07∶00, lights off at 19∶00), with free access to food and water. All experiments were performed during the light period, when the mice were 8–12 weeks of age.

Edamura M., Murakami G., Meng H., Itakura M., Shigemoto R., Fukuda A, & Nakahara D. (2014). Functional Deficiency of MHC Class I Enhances LTP and Abolishes LTD in the Nucleus Accumbens of Mice. PLoS ONE, 9(9), e107099.

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3

Murine Models for Immunological Research

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C57BL/6 and nude mice were purchased from the Shanghai Laboratory Animal Center of Chinese Academy of Sciences, Shanghai, China, and maintained under specific pathogen-free conditions. Rag1^−/− mice, gld/gld mice, CIITA^−/− mice and β2m^−/− mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA). Mice were housed in the Vivarium of Shanghai Jiao Tong University School of Medicine. All procedures were approved by the Institutional Animal Care and Use Committee of the Institute of Health Sciences, Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences. Animals were matched for age and gender in each experiment.

Cao K., Wang G., Li W., Zhang L., Wang R., Huang Y., Du L., Jiang J., Wu C., He X., Roberts A.I., Li F., Rabson A.B., Wang Y, & Shi Y. (2015). Histone deacetylase inhibitors prevent activation-induced cell death and promote anti-tumor immunity. Oncogene, 34(49), 5960-5970.

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4

Generation of ERAAP Knockout Mice

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ERAAP^−/− mice (B6.129.Arts1^tm1Ucb) have been previously described. ERAAP^−/−K^bD^b−/−, ERAAP^−/−TAP^−/−, ERAAP^−/−β₂m^−/− and ERAAP^−/− Qa-1^{b −/−} mice were generated in our facility by crossing the respective knockout mice with ERAAP^−/− mice. WT C57Bl/6J, and β₂m^−/− mice were either purchased from the Jackson Laboratory (Bar Harbor, ME) or bred in our facility. Qa-1^{b −/−} mice were generated in the laboratory of Dr. Harvey Cantor (Harvard University) and were the kind gift of Dr. Edgar Engleman (Stanford University). Mice were housed and all procedures carried out with the approval of, and in accordance with, the institutional animal care guidelines of the University of California Berkeley and the University of Montreal.

Nagarajan N.A., de Verteuil D.A., Sriranganadane D., Yahyaoui W., Thibault P., Perreault C, & Shastri N. (2016). ERAAP shapes the peptidome associated with classical and non-classical MHC class I molecules. Journal of immunology (Baltimore, Md. : 1950), 197(4), 1035-1043.

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5

Generating Genetically Modified Mouse Models

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K^bD^b−/− mice were purchased from Taconic Biosciences and then maintained in-house. B6.SJL mice (Taconic) were crossed with K^bD^b−/− mice to obtain congenic K^bD^b−/− (K^bD^b−/−.SJL) animals. For certain experiments, K^bD^b−/− and K^bD^b−/−.SJL mice were used interchangeably as K^bD^b−/−. CD1d^−/− mice (Jackson Laboratory) and RAG1^−/− mice (Jackson Laboratory) were crossed with K^bD^b−/− mice to obtain CD1d^−/−K^bD^b−/− and RAG1^−/−K^bD^b−/− animals, respectively. C57BL/6 and β₂m^−/− mice were obtained from Jackson Laboratory. C57BL^/6 mice were used as wild-type controls. None of the experiments within these studies were blinded or randomized. Both age- and sex-matched mice were used for this study, which was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals, as defined by the National Institutes of Health (PHS Assurance #A3284–01). Animal protocols were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of Brown University. All animals were housed in a centralized and AAALAC-accredited research animal facility that is fully staffed with trained husbandry, technical, and veterinary personnel.

Anderson C.K., Reilly E.C., Lee A.Y, & Brossay L. (2019). Qa-1-Restricted CD8+ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection. Cell reports, 27(2), 537-548.e5.

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6

Transgenic Mouse Protocols for Research

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Animals were used under protocols approved by local institutional research committees and in accordance with UK Home Office guidelines. C57BL/6 (B6) and CD45.1.OT-I TCR transgenic.Rag2^−/− mice were bred in-house. CD11c.DTR and β2M^−/− mice (on the B6 background) were bought from Jackson Laboratories and bred in-house.

Hotblack A., Seshadri S., Zhang L., Hamrang-Yousefi S., Chakraverty R., Escors D, & Bennett C.L. (2017). Dendritic Cells Cross-Present Immunogenic Lentivector-Encoded Antigen from Transduced Cells to Prime Functional T Cell Immunity. Molecular Therapy, 25(2), 504-511.

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β2m mice

Lab products found in correlation

Spelling variants of this product

6 protocols using β2m mice

Generating Genetically Modified Mouse Models

Behavioral analysis of β2m-/- mice

Murine Models for Immunological Research

Generation of ERAAP Knockout Mice

Generating Genetically Modified Mouse Models

Transgenic Mouse Protocols for Research

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