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Anti p16 ink4a

Manufactured by Proteintech
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Anti-p16-INK4A is a laboratory reagent used in research applications. It is an antibody that specifically binds to the p16-INK4A protein, which plays a role in cell cycle regulation. This product can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to detect and analyze the expression of p16-INK4A in biological samples.

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Lab products found in correlation

Anti akt, by Cell Signaling Technology (2 mentions) Anti kras sc30, by Santa Cruz Biotechnology (1 mentions) Anti nras sc 519, by Santa Cruz Biotechnology (1 mentions) Anti phospho mek, by Cell Signaling Technology (1 mentions) Ripa buffer, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp conjugated anti rabbit igg, by Merck Group (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Anti phospho erk, by Cell Signaling Technology (1 mentions) Anti phospho akt, by Cell Signaling Technology (1 mentions) Anti mek, by Cell Signaling Technology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Fugene hd transfection reagent, by Promega (1 mentions) Anti nqo1, by Cell Signaling Technology (1 mentions) Anti p eif2α ser51, by Cell Signaling Technology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti p27, by Santa Cruz Biotechnology (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Anti cyclin d1, by Santa Cruz Biotechnology (1 mentions) Anti p21, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-P16 (12 citations) P16-INK4A (11 citations) Rabbit anti-P16-INK4A (4 citations) Anti-TM (2 citations)

4 protocols using anti p16 ink4a

1

Transfection and Western Blot Analysis

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Hep3B cells were transfected with 2μg of plasmid DNA using FuGENE HD Transfection Reagent (Promega, Madison, WI, USA), according to the manufacturer's instructions. Two days post transfection, cells were lysed in 1× RIPA buffer (#9806; Cell Signaling Technology, Danvers, MA, USA). Western blot experiments were performed using standard methods. Anti-HRAS (sc-520; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-KRAS (sc-30; Santa Cruz Biotechnology), anti-NRAS (sc-519; Santa Cruz Biotechnology), anti-AKT (#9272, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-AKT (#4060, Cell Signaling Technology), anti-MEK (#9126, Cell Signaling Technology), anti-phospho-MEK (#9154, Cell Signaling Technology), anti-ERK (#9102, Cell Signaling Technology), anti-phospho-ERK (#4370, Cell Signaling Technology), anti-p16INK4A (10883-1-AP; Proteintech, Chicago, IL, USA), anti-GAPDH (#2118; Cell Signaling Technology), and anti-β-actin (sc-47778; Santa Cruz Biotechnology) were used as primary antibodies, and horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (A0545;Sigma-Aldrich, St. Louis, MO, USA) was used as the secondary antibody.
Chung S.I., Moon H., Ju H.L., Kim D.Y., Cho K.J., Ribback S., Dombrowski F., Calvisi D.F, & Ro S.W. (2016). Comparison of liver oncogenic potential among human RAS isoforms. Oncotarget, 7(6), 7354-7366.
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2

Western Blot Analysis of Cell Lysates

Cited 1 time
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Cells were harvested and lysed and protein content of cell lysates was measured as described previously [26 (link)] Whole cell lysates were separated by SDS-PAGE (in 10% or 15% SDS-polyacrilamide gel). SDS-PAGE and Western Blot analysis was done according to our previous study [26 (link)]The following antibodies were applied: anti-NQO1 (Cell Signaling, A180), anti-CyclinD1 (Santa Cruz, A-12), anti-p21 (Santa Cruz, C-19), anti-p27 (Santa Cruz, C-19), anti-p15-INK4b (proteintech, 12877-1-AP), anti-p16-INK4A (proteintech, 10883-1-AP), anti-PERK (Cell Signaling, 3192S), anti-P-eIF2α (Cell Signaling, 9722S9), anti-P-eIF2α (Ser51) (Cell Signaling, 9721L), and anti-GAPDH (Santa Cruz, 6C5) and HRP conjugated secondary antibodies (SantaCruz, sc-2020 and Cell Signaling, 7074S, 7076S).
Márton M., Tihanyi N., Gyulavári P., Bánhegyi G, & Kapuy O. (2018). NRF2-regulated cell cycle arrest at early stage of oxidative stress response mechanism. PLoS ONE, 13(11), e0207949.
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3

Antibody Panel for Cellular Analysis

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Antibodies used for experiments included anti-GFP (1:1000; sc-9996; Santa Cruz Biotechnology, Dallas, TX, USA); anti-GST (1:5000; sc-138; Santa Cruz Biotechnology), anti-His (1:1000; 66005-1-lg; Proteintech, Rosemont, IL, USA), anti-Actin (1:10000; sc-47778; Santa Cruz Biotechnology), anti-Lamin A/C (1:10000; sc-376248; Santa Cruz Biotechnology), anti-Progerin (1:100; sc-81611; Santa Cruz Biotechnology), anti-Progerin (1:300; ab66587; Abcam, Cambridge, UK), anti-Ki67 (1:200; Ab15580; Abcam), anti-cyclin B1 (1:100; sc-594; Santa Cruz Biotechnology), and anti-p16-INK4A (1:500; 10883-1-AP; Proteintech).
Kang S.M., Yoon M.H., Ahn J., Kim J.E., Kim S.Y., Kang S.Y., Joo J., Park S., Cho J.H., Woo T.G., Oh A.Y., Chung K.J., An S.Y., Hwang T.S., Lee S.Y., Kim J.S., Ha N.C., Song G.Y, & Park B.J. (2021). Progerinin, an optimized progerin-lamin A binding inhibitor, ameliorates premature senescence phenotypes of Hutchinson-Gilford progeria syndrome. Communications Biology, 4, 5.
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4

Western Blot Analysis of Signaling Proteins

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Whole cell lysates were separated on a tris-glycine gel (8–16%, Thermo Fisher Scientific), transferred onto a poly-vinylidene difluoride membrane (Bio-Rad), and incubated overnight with the following primary antibodies: anti-phosphorylated Akt (Ser473 rabbit monoclonal, clone D9E; Cell Signaling Technologies); anti-Akt (rabbit polyclonal, cone 11e7 Cell Signalling Technologies); anti-phosphorylated IκBα (Ser32/36 mouse monoclonal, clone 5A5, Cell Signalling Technologies); anti total-IκBα (mouse monoclonal, clone 112B2, Cell Signalling Technologies); and anti-p16INK4a (rabbit polyclonal, Proteintech). The membrane was incubated with goat anti-rabbit or anti-mouse HRP-conjugated secondary antibodies (sc-2030 and sc-2005, respectively; Santa Cruz Biotechnology). Bands were detected by Clarity Western ECL Substrate (Bio-Rad), imaged using Alliance LD2 system and software (UVItec), and quantified by densitometry analysis with ImageJ software (NCBI). Values were normalized against those of GAPDH, as revealed by a primary antibody directly conjugated to HRP (clone D16H11, Cell Signalling Technologies).
Lazzarini E., Balbi C., Altieri P., Pfeffer U., Gambini E., Canepa M., Varesio L., Bosco M.C., Coviello D., Pompilio G., Brunelli C., Cancedda R., Ameri P, & Bollini S. (2016). The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity. Scientific Reports, 6, 29994.
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