PEG2k-NH2 was purchased from Xiamen SINOPEG Biotechnology Co., Ltd. (Xiamen, China). Methylene chloride, dimethyl sulfoxide (DMSO), N, N-dimethylformamide (DMF), dichloromethane (DCM), trichloromethane (TCM), diethyl ether, and hydrogen peroxide were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). Succinic anhydride 1-ethyl-3(3-dimethylpropylamine) (EDCI) was offered by Beijing Bailingwei Technology Co., Ltd. (Beijing, China). Hexadecyl isocyanate and MTT were obtained from Sigma-Aldrich (Shanghai) Trading Co., Ltd. (Shanghai, China). Carboplatin was obtained from Shandong Boyuan Pharmaceutical Co., Ltd. (Jinan, China). St. Nile red and phosphotungstic acid were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). RPMI1640 culture medium and newborn fetal bovine serum were obtained from GIBCO Biotechnology Co., Ltd. (Carlsbad, CA). H460, A549 tumor cell lines were obtained from the Institute of Basic Medicine, Chinese Academy of Medical Sciences (Beijing, China). SPF balb/c/nu/nu nude mice were provided by Viton Lihua.
Methylene chloride
Manufactured by Sinopharm
Sourced in China
Methylene chloride is a colorless, volatile, and highly dense organic solvent. It has a wide range of applications in various industries, including chemical synthesis, paint stripping, and metal degreasing. Methylene chloride is also used as a laboratory reagent and in certain medical applications. Its core function is to serve as a versatile solvent for a variety of materials.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Sinopharm (4 mentions)
Methanol, by Sinopharm (3 mentions)
Phosphotungstic acid, by Sinopharm (2 mentions)
Sodium hydroxide (naoh), by Sinopharm (2 mentions)
Phosphoric acid, by Sinopharm (2 mentions)
Hexadecyl isocyanate, by Merck Group (1 mentions)
Trichloromethane, by Sinopharm (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Dichloromethane, by Sinopharm (1 mentions)
Hydrogen peroxide, by Sinopharm (1 mentions)
Diethyl ether, by Sinopharm (1 mentions)
Dimethylformamide, by Sinopharm (1 mentions)
Cupric sulfate, by Sinopharm (1 mentions)
Epichlorohydrin, by Dow (1 mentions)
N n diisopropylethylamine, by J&K Scientific (1 mentions)
4 4 diphenylmethane diisocyanate, by J&K Scientific (1 mentions)
Triethylamine, by Sangon (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Triethylamine tea, by Sinopharm (1 mentions)
10 protocols using methylene chloride
1
Synthesis and Evaluation of PEG-Modified Nanocarrier
2
Synthesis and Characterization of PEG-based Antimicrobial
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Epichlorohydrin (Dow Chemical, USA), trifluoride diethyl etherate (BF3–OEt2, Tianjin Lianxing Biotechnology Co. Ltd.), 3-bromo-1-propyne (Aladdin, Shanghai), sodium ascorbate (VcNa, Aladdin, Shanghai), trypticase soy agar medium (TSA, AOBOX, Hengshui), trypticase soy liquid medium (TSB, AOBOX, Hengshui), 1,4-butylene glycol (BDO, KESHI, Chengdu), 4,4′-diphenylmethane diisocyanate (MDI, J&K, Beijing), tetrahydrofuran (THF, Bioway, Shanghai), anhydrous magnesium sulfate (MgSO4, Tianjin ZhiYuan Reagent Co. Ltd.) N,N-diisopropylethylamine (DIEA, J&K, Beijing) can be used directly. All other chemical reagents such as sodium hydride (NaH), dimethyl sulfoxide (DMSO), sodium azide (NaN3), methanol, sodium hydroxide (NaOH), diethyl ether, cupric sulfate (CuSO4), phosphate buffer (PBS), polypropylene glycol (PPG), methylene chloride (DCM) and dimethylformamide (DMF) were purchased from Sinopharm. S. aureus and E. coli were purchased from Guangdong Institute of Microbiology. mPEG with number average molecular weights of 5000, 3350 and 1000 (mPEG-5000, mPEG-3350 and mPEG-1000, Aladdin) were used without further purification.
3
Rare Earth Oxide Nanoparticle Synthesis
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Rare earth oxides Y2O3 (99.99%), Yb2O3 (99.99%), and Er2O3 (99.99%) were obtained from China Rare Earth Online Co. Ltd. Rare earth chlorides (LnCl3, Ln: Y, Gd, Yb, Er) were prepared by dissolving the corresponding oxides in hydrochloric acid and then evaporating the water completely. Ammonium fluoride (NH4F, 98%), sodium hydroxide, hydrochloric acid, ethanol, methanol, methylene chloride, triethylamine (TEA), and dimethyl sulfoxide (DMSO) were purchased from Sinopharm Chemical Reagent Co., Ltd (China). Oleic acid (OA, 90%), 1-octadecene (ODE, 90%), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich. Anticancer drug doxorubicin hydrochloride (DOX, > 98%) was purchased from Shanghai Sangon Biotech Company (Shanghai China) and was deprotonated with addition of triethylamine to obtain the hydrophobic form. Carboxyl-terminated Poly(lactide-co-glycolide) (PLGA-COOH 50:50, Mw = 10000) was obtained from Jinan Daigang Bioengineer Company, China. Polyvinyl alcohol (PVA, Mw = 22000) was obtained from Kuraray CO. LTD, Japan. All reagents were used as received without further purification.
4
Synthesis of 1,2,4-Triaminobenzene
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The compound 1,2,4-triaminobenzene was purchased from Wuhan Belka Biomedical Co., Ltd. (Wuhan, China). Methanol and nitric acid were from Guangdong Guanghua Sci-Tech Co., Ltd. (Guangdong, China). Formamide was from Shandong Xiya Reagent Co., Ltd. (Shandong, China). Methylene chloride was from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Column Chromatography Silica Gel 200–300 mesh was from Qingdao Ocean Chemical Co., Ltd. (Qingdao, China). All reagent were of analytical grade. Ultrapure water (Milli-Q, Merck, Darmstadt, Germany) was used for the preparation of the solutions. The food storage box was purchased from Yiwu Ouxing E-Commerce Co., Ltd. (Yiwu, China).
5
Synthesis of Biofuel Precursors
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Terephthalic acid was purchased from Aladdin Industrial Corporation. 5-Hydroxymethylfurfural (HMF, 98%), 5-ethoxymethylfurfural (EMF, 97%), ethyl levulinate (EL, 99%), and zirconium octahydrate oxychloride were purchased from Macklin Biochemical Co., Ltd. Ethanol (≥99.8%), glacial acetic acid, N, N-dimethylformamide (DMF), and methylene chloride were obtained from Sinopharm Chemical Reagent Co., Ltd. Chlorosulfonic acid was purchased from Shanghai Jiuding Chemical Reagent Co., Ltd.
6
Phenanthrene and Cd(NO3)2 Removal
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Phenanthrene and Cd(NO3)2, nitric acid, sulfuric acid, methanol, methylene chloride, ethanol, rhamnolipid, sodium chloride and sodium hydroxide were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All reagents were of analytical purity grade. Deionized water used in this study was obtained from a Millipore Milli-Q system.
7
Preparation of HER2-targeted PLGA Nanoparticles
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PLGA (50:50; molecular weight, 40,000) was obtained from Jinan Daigang Biomaterial Co., Ltd. (Shandong, China). Polyvinyl alcohol (PVA; 88% mole hydrolyzed) and (D+)-camphor were purchased from Aladdin Chemistry Co., Ltd (Shanghai, China). Methylene chloride, isopropanol, mannitol and phosphotungstic acid were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Fluorescein isothiocyanate (FITC)-conjugated rabbit anti-human HER2 monoclonal antibody and control rat immunoglobulin G (IgG) antibody were synthesized by BD Biosciences (Palo Alto, CA, USA). Phycoerythrin (PE)-conjugated rat anti-mouse VEGFR2 monoclonal antibody was synthesized by Shanghai Jinmai Biotechnology Co., Ltd (Shanghai, China). C3F8 gas was purchased from Shanghai Renjie Ling Optics Instrument Co., Ltd (Shanghai, China).
8
Baicalin Anti-Inflammatory Compound Characterization
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Baicalin active pharmaceutical ingredients (API) (Xi'an Baikang Biotechnology Co., Ltd., Xi'an, China); Baicalin Control (China Academy of Food and Drug Administration, Beijing, China); Methanol (Shanghai McLean Biotechnology Co., Ltd., Shanghai, China); Polyvinyl Alcohol (Chengdu West Asia Chemical Co., Ltd., Chengdu, China); Ethyl Cellulose (Chengdu West Asia Chemical Co., Ltd., Chengdu, China); Methylene Chloride (Sinopharm Group Chemical Reagent Co. Ltd., Shanghai, China); phosphoric acid (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China); foetal bovine serum (Biological Industries, USA); sodium bicarbonate (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China); trichloromethane (Tianjin YD Chemical Reagent Co., Ltd., Tianjin, China); methanol (Tianjin YD Chemical Reagent Co. Ltd., Tianjin, China); ethanol (Tianjin Yongda Chemical Reagent Co., Ltd., Tianjin, China); isopropanol (Tianjin Yongda Chemical Reagent Co., Ltd., Tianjin, China); IL-1β kit (Thermo Fisher Scientific, USA); TNF-α kit (Thermo Fisher Scientific, USA); ELISA reagents (SINOPACIFIC Group Chemical Reagent Co. Ltd., Shanghai, China); ELISA kit (Thermo Fisher Scientific, USA); ultrapure water (Speed Instrument Technology Co., Ltd., Shanghai, China).
9
Reagent-Grade Synthesis of Fluorescent Polymer
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Reagent grade of o-phenylenediamine (oPD), L-aspartic acid (AA) and folic acid (FA) were bought from Aladdin Chemicals Co. Ltd (Shanghai, China). Citric acid, ethanolamine, ethanediamine (EDA), polyvinyl alcohol (PVA), methylene chloride, ethanol, phosphoric acid and urea were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Pure cyanuric acid (pCA) was purchased from J&K Chemical Reagent Co. Ltd (Beijing, China). All chemicals were used as received without further purification unless otherwise specified. Deionized (DI) water was used throughout this study.
10
In Vitro and In Vivo Evaluation of TH-302 Loaded PLGA Nanoparticles
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TH-302 was purchased from Abmole Bioscience Inc (Shanghai, China). PLGA polymers were purchased from Jinan Daigang Biomaterial Co., Ltd (Jinan, Shandong Province, China). PVA-124, methanol, methylene chloride, Tween 20/80, and other chemical reagents were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Dulbecco’s modified Eagle’s medium (DMEM), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and fetal bovine serum (FBS) were purchased from Gibco Life Technology Company (Grand Island, NY). Microguide wire (MAK501N) was purchased from Merit Medical System Inc (South Jordan, UT). Micro-catheter (STD130-22S) was purchased from ASAHI INTECC Co., Ltd. (Tokyo, Japan). A venous indwelling needle (18G) was purchased from B. Braun Medical Inc. (Melsungen, Germany). The contrast agent Ultravist was purchased from Bayer HealthCare Co., Ltd. (Leverkusen, Germany). Hydroxylamine hydrochloride and ketamine hydrochloride were provided by the experimental animal center of the Chinese PLA General Hospital.
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