Fl amg

Following a 24 h cotreatment of LPS (100 μg), cells were incubated with a 3 μM tetraethyl-benzimidazolyl-carbocyanine iodide (JC-1) staining solution (T3168, Invitrogen, Waltham, MA, USA) at 37 °C for 20 min and then washed with PBS in order to visualize the fluorescence with a fluorescence microscope (EVOS Fl AMG). The JC-1 probe aggregates formed a polymer in the mitochondrial matrix of healthy cells, producing a strong red fluorescence (Ex  = 585 nm, Em  =  590 nm). Otherwise, dysfunctional mitochondria presented JC-1 monomers, resulting in the emission of a green fluorescent signal (Ex  =  514 nm, Em =  529 nm). The results are expressed as the ratio of red/green fluorescence.

Following a 6h co-treatment of FFA 0.75 mM and combinations of OLE and ROE, cells were incubated with a 3 μM JC-1 staining solution (T3168, Invitrogen, Waltham, MA, USA) at 37 °C for 20 min and then washed with PBS in order to visualize the fluorescence with a fluorescence microscope (EVOS Fl AMG). The JC-1 probe aggregates to form a polymer in the mitochondrial matrix of healthy cells, producing a strong red fluorescence (Ex = 585 nm, Em = 590 nm). Otherwise, dysfunctional mitochondria present JC-1 monomers, resulting in the emission of a green fluorescent signal (Ex = 514 nm, Em = 529 nm). The results are expressed as the ratio of red/green fluorescence.