Vantera analyzer

Hemoglobin and hematocrit were measured in EDTA-anticoagulated whole blood (Sysmex XE-5000).12 (link) Insulin was measured in serum and glucose, cholesterol, TG, HDL in plasma using a Roche Cobas 6000 analyzer (Roche Diagnostics, Indianapolis, Indiana). LDL was calculated using the Friedewald equation.21 (link) A1C values were determined by HPLC using National Glycohemoglobin Standardization Program-certified instruments manufactured by BioRad Laboratories (Hercules, California, USA). Size of HDL and LDL particles were determined by NMR using the Lp3 algorithm on the Vantera Analyzer (LabCorp, Burlington, North Carolina, USA).

Disposition Index (DI)25 (link) :
${\displaystyle \mathrm{I}\mathrm{n}\mathrm{s}\mathrm{u}\mathrm{l}\mathrm{i}\mathrm{n}\mathrm{s}\mathrm{e}\mathrm{n}\mathrm{s}\mathrm{i}\mathrm{t}\mathrm{i}\mathrm{v}\mathrm{i}\mathrm{t}\mathrm{y}\mathrm{i}\mathrm{n}\mathrm{d}\mathrm{e}\mathrm{x}\ast \frac{\mathrm{A}\mathrm{U}\mathrm{C}\mathrm{i}\mathrm{n}\mathrm{s}\mathrm{u}\mathrm{l}\mathrm{i}\mathrm{n}}{\mathrm{A}\mathrm{U}\mathrm{C}\mathrm{g}\mathrm{l}\mathrm{u}\mathrm{c}\mathrm{o}\mathrm{s}\mathrm{e}}}$
Glucose, insulin, liver enzymes were analyzed in serum with Roche Cobas 6000 analyzer (Roche Diagnostics, Indianapolis, Indiana, USA).
Cholesterol, triglycerides (TG) and direct high-density lipoprotein cholesterol (HDL-C) were measured enzymatically on the Cobas 6000 analyzer (Roche Diagnostics). Low-density lipoprotein cholesterol (LDL-C) was calculated using the Friedewald equation.26 (link) LDL particle number, and HDL and LDL size were measured by nuclear magnetic resonance (NMR) on the Vantera analyzer, using the Lp3 algorithm (LabCorp).
Hemoglobin, hematocrit, white cells and mean corpuscular volume (MCV) were measured in EDTA-anticoagulated whole blood using a Sysmex XE-5000 analyzer (Chicago, Illinois, USA). Iron, transferrin and ferritin were measured in serum (Roche Cobas 6000 analyzer).