HCC cells were treated with CDDP or doxorubicin for 24 h. Apoptotic cells were detected with the In Situ Cell Death Detection Kit (Roche, Mannheim, Germany) according to the manufacturer' protocol, followed counterstained with 4′, 6 -diamidino-2-phenylindole. Apoptotic cells were imaged with a Nikon microscope with NIS Element F3.2 software (Nikon, Tokyo, Japan).
Nis element f3
Manufactured by Nikon
Sourced in United States
NIS Element F3.2 is a comprehensive imaging software solution designed for a wide range of microscopy applications. It offers a flexible and user-friendly interface for image acquisition, processing, and analysis. The software supports various types of microscopes, including fluorescence, confocal, and live-cell imaging systems.
Automatically generated - may contain errors
Lab products found in correlation
In situ cell death detection kit, by Roche (1 mentions)
Alexa fluor 647 conjugated affinipure donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Alexa fluor 488 conjugated affinipure donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Tcs sp5 confocal microscope, by Leica (1 mentions)
4 protocols using nis element f3
1
Apoptosis Detection in HCC Cells
2
Immunofluorescence of N-Cadherin in HCC
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Stable HCC cell lines mis-expressing Prp19 were incubated with antibody against N-cadherin and then incubated with anti-mouse IgG (Jackson ImmunoResearch Laboratories). The coverslips were counterstained with DAPI and imaged with a Nikon microscope with NIS Element F3.2 software (Nikon, Melville, NY, USA).
3
Immunohistochemical Analysis of HCC Markers
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Slides of paraffin-embedded primary HCC and adjacent paratumor tissues were used for immunochemistry and stained with antibodies against p-p38, Prp19 and Twist1, E-cadherin and N-cadherin. The exact procedure and the semi-quantitative method were reported elsewhere [15 (link)]. Twenty-nine samples from 169 HCC cases were scored as 0. The optimum cut-off value for immunoactivity score was defined by receiver operating curve analysis as described in Supplementary Table S3 . Images were processed with a Nikon microscope with NIS Element F3.2 software (Nikon, Melville, NY, USA).
4
Immunofluorescence Staining of Prp19 and Cdc5L in HCC
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Slides of paraffin-embedded primary HCC tissues were used for immunochemistry and stained with antibodies against Prp19 and Cdc5L. The exact procedure and the semi-quantitative method were reported elsewhere [27 (link)]. Images were processed with a Nikon microscope with NIS Element F3.2 software (Nikon, Melville, NY, USA).
When subjected to immunofluorescence assay, unfixed frozen sections were generally processed as described below, briefly. First, sections were incubated with rabbit anti-human Prp19 polyclonal antibody (1:250) and mouse anti-human Cdc5L Monoclonal antibody (1:20) overnight at 4 °C after blocked by Phosphate buffered saline (PBS) containing 5% Bovine serum albumin (BSA). They were then washed by PBS carefully. These sections were incubated with Alexa Fluor® 647-conjugated AffiniPure Donkey Anti-Rabbit IgG and Alexa Fluor® 488-conjugated AffiniPure Donkey Anti-Mouse IgG (both 1:500) at room temperature for 2 h (Jackson ImmunoResearch Inc., West Grove, PA, USA). Finally, sections were added with Mounting Medium for Flurescence with DAPI and detected by Leica TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany).
When subjected to immunofluorescence assay, unfixed frozen sections were generally processed as described below, briefly. First, sections were incubated with rabbit anti-human Prp19 polyclonal antibody (1:250) and mouse anti-human Cdc5L Monoclonal antibody (1:20) overnight at 4 °C after blocked by Phosphate buffered saline (PBS) containing 5% Bovine serum albumin (BSA). They were then washed by PBS carefully. These sections were incubated with Alexa Fluor® 647-conjugated AffiniPure Donkey Anti-Rabbit IgG and Alexa Fluor® 488-conjugated AffiniPure Donkey Anti-Mouse IgG (both 1:500) at room temperature for 2 h (Jackson ImmunoResearch Inc., West Grove, PA, USA). Finally, sections were added with Mounting Medium for Flurescence with DAPI and detected by Leica TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany).
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