Hepm 1 media kit

Example 2

The human NB cell lines, SK-N-DZ, SK-N—BE(2)c, SK-N-AS, and LAN-5 obtained from the American Type Culture Collection (Rockville, Md.), were cultured in DMEM with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin in the presence of 5% CO₂ at 37° C. Human PBMCs from a healthy donor were purchased from Sanguine BioSciences (Valencia, Calif.) and grown in RPMI1640 with 10% FBS, 100 units/ml penicillin, 100 μg/ml streptomycin, and 10 ng/ml IL-2 in the presence of 5% CO₂ at 37° C. Human embryonic progenitor cell line 7SM0032 was acquired from Millipore (Billerica, Mass.) and grown in the hEPM-1 Media Kit purchased from the same company. The plasmid pCBASce expresses the rare cutting I-SceI meganuclease [22]. The U20S-derived cell lines, DR-GFP and EJ5-GFP, each contain a stably transfected reporter gene for DSB repair mediated by HR and end joining (EJ), respectively [23]. These cell lines were cultured in DMEM medium with 10% FBS at 37° C. in the presence of 5% CO₂.

Example 2

The human NB cell lines, SK-N-DZ, SK-N-BE(2)c, SK-N-AS, and LAN-5 obtained from the American Type Culture Collection (Rockville, Md.), were cultured in DMEM with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin in the presence of 5% CO₂ at 37° C. Human PBMCs from a healthy donor were purchased from Sanguine BioSciences (Valencia, Calif.) and grown in RPMI1640 with 10% FBS, 100 units/ml penicillin, 100 μg/ml streptomycin, and 10 ng/ml IL-2 in the presence of 5% CO₂ at 37° C. Human embryonic progenitor cell line 7SM0032 was acquired from Millipore (Billerica, Mass.) and grown in the hEPM-1 Media Kit purchased from the same company. The plasmid pCBASce expresses the rare cutting I-SceI meganuclease [22]. The U20S-derived cell lines, DR-GFP and EJ5-GFP, each contain a stably transfected reporter gene for DSB repair mediated by HR and end joining (EJ), respectively [23]. These cell lines were cultured in DMEM medium with 10% FBS at 37° C. in the presence of 5% CO₂.

Example 2

The human NB cell lines, SK-N-DZ, SK-N-BE(2)c, SK-N-AS, and LAN-5 obtained from the American Type Culture Collection (Rockville, Md.), were cultured in DMEM with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin in the presence of 5% CO₂ at 37° C. Human PBMCs from a healthy donor were purchased from Sanguine BioSciences (Valencia, Calif.) and grown in RPMI1640 with 10% FBS, 100 units/ml penicillin, 100 μg/ml streptomycin, and 10 ng/ml IL-2 in the presence of 5% CO₂ at 37° C. Human embryonic progenitor cell line 7SM0032 was acquired from Millipore (Billerica, Mass.) and grown in the hEPM-1 Media Kit purchased from the same company. The plasmid pCBASce expresses the rare cutting I-SceI meganuclease [22]. The U2OS-derived cell lines, DR-GFP and EJ5-GFP, each contain a stably transfected reporter gene for DSB repair mediated by HR and end joining (EJ), respectively [23]. These cell lines were cultured in DMEM medium with 10% FBS at 37° C. in the presence of 5% CO₂.