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129svev 129 mice

Manufactured by Taconic Biosciences

The 129Svev [129] mice are a widely used inbred mouse strain. These mice are genetically engineered and serve as a model organism in various biomedical research applications.

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2 protocols using 129svev 129 mice

1

Behavioral Effects of Toxoplasma gondii Infection in Mice

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Mice used in this study were the male F1 progeny of a cross between C57BL/6NtacfBr [C57B6] and 129Svev [129] mice (Taconic Farms, Germantown, NY). Mice were bred in our colony at The Hospital for Sick Children and maintained on a 12 h light/dark cycle with access to food and water ad libitum. A total of 39 mice were used for behavioral experiments with T. gondii CEP, 40 mice were used for behavioral experiments with T. gondii VEG and an additional cohort of mice were used for the RNA-seq experiments, a subset of which were infected with T. gondii CEP; one mouse treated with T. gondii CEP and cocaine died prior to sampling.
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2

Strain-Specific Effects of Nicotine on Learning

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Male A/J (A; n = 19), BALB/cByJ (BALB; n = 18), C3H/HeJ (C3H; n = 18), C57BL/6J (B6; n = 18), CBA/J (CBA; n = 18), DBA/1J (D1; n = 20), and DBA/2J (D2; n = 18) mice were obtained from Jackson Laboratory (Bar Harbor, ME). 129/SvEv (129) mice were obtained from Taconic Farms (Germantown, NY). These inbred strains were chosen because previous work from our lab found phenotype differences in these strains for the effects of nicotine on learning and memory (Portugal, Wilkinson, Kenney, Sullivan, & Gould, 2012 (link)). Mice were trained at 8–10 weeks of age. Mice were housed in groups of three per cage and maintained on a 12-hr light–dark cycle (lights on 7:00 a.m.) with unrestricted access to food and water. Experiments took place during the light phase. All behavioral and surgical procedures were approved by the Temple University Institutional Animal Care and Use Committee.
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