8 arm peg

8-arm PEG (MW ∼ 40 kDa) was purchased from JenKem Technology USA (Allen, TX, USA). Linear PEG (MW ∼ 1.5 kDa) was purchased from Sigma-Aldrich USA (St. Louis, MO, USA). RGD peptide (CRGDS) was purchased from Bio Basic, Inc (Amherst, NY, USA). Sodium hyaluronate (HA) (MW ∼ 20–40 kDa) was purchased from Lifecore Biomedical (Chaska, MN, USA). MMP-degradable peptide (KCGPQGIWGQCK) was purchased from GenScript (Piscataway, NJ, USA). 8-arm PEG-norbornene (PEG-NB) and Linear PEG-dithiol (PEGSH) were synthesized as previously reported [24 (link),25 (link)]. Thiolated sodium hyaluronate (HA-SH) was synthesized as previously reported [26 (link)]. Alginate was purchased from Sigma (St. Louis, MO, USA). Hydrolytically degradable Alginate was synthesized as previously reported [22 ]. All other reagents were obtained from Fisher Scientific (Pittsburgh, PA, USA) unless otherwise noted.

PEG (molecular weight (MW) 2 kDa, 4 kDa), K₂CO₃, dichloromethane, acryloyl chloride, KI, Celite® 521, 4-(dimethylamino)-pyridine, N,N′-diisopropylcarbodiimide, 5-norbornene-2-carboxylic acid, NaH, allyl bromide, 2,2-dimethoxy-1,2-diphenylethan-1-one, dithiothreitol, tetrahydrofuran, and thioacetic acid were purchased from Sigma-Aldrich (St Louis, MO, USA). 4-(2-Hydroxyethoxy)phenyl-(2-hydroxy-2-propyl)ketone (Irgacure 2959) was purchased from BASF (Florham Park, NJ, USA). PEGDA (MW 5 kDa) was purchased from Laysan Bio (Arab, AL, USA). Four-arm PEG (MW 5 kDa), 4-arm PEG-thiol (MW 5 kDa), 8-arm PEG (MW 10 kDa), and 8-arm PEG-thiol (MW 10 kDa) were purchased from JenKem Technology (Allen, TX, USA). PEGDA (MW 2 kDa, 4 kDa), 8-arm PEG-norbornene (MW 10 kDa), 4-arm PEG-norbornene (MW 5 kDa), and PEG-dithiol (MW 1.5 kDa) were synthesized in house. BSA was purchased from Fisher Scientific (Pittsburg, PA, USA). The BioRad Protein Assay was purchased from BioRad (Hercules, CA, USA).