Anti mct2

The BCA kit, DA (rat) kit, β-hydroxybutyric acid (β-HB; rat) kit, acetoacetic acid (AcAc; rat) kit, and citrate synthase (CS; rat) kit were purchased from Sangon Biotech (Shanghai, China). The ATP assay kit, mitochondrial complex I assay kit, glucose kit, and hexokinase (HK) kit were purchased from Solarbio (Beijing, China). Scutellariae Radix, licorice, Paeoniae Radix Alba, and Jujubae Fructus were purchased from the Beijing Tongren drug store (Beijing, China). Rotenone was purchased from Target Mol (USA) and madopar was purchased from Shanghai Roche Pharmaceuticals Ltd. D2O was purchased from Norell (Landisville, PA, USA). Sodium 3-trimethylsilyl 2,2,3,3-d4 propionate (TSP) was purchased from Cambridge Isotope Laboratories Inc. (Andover, MA, USA). Primary antibodies for GLUT1, MCT1, and GAPDH were purchased from Proteintech Group Co., Ltd. (Chicago, IL), and anti-rabbit lgG/HRP and anti-MCT2 were purchased from Bioss, Inc.

The following antibodies were used in the experiments: Anti-NBCe1 and Anti-NBCe2 (Origene, Rockville, MD), Anti-NBCn1 (Abgent, San Diego, CA), Anti-NDCBE (Aviva Systems Biology, San Diego, CA). Anti-NHE1 and Anti-NHE2 (Origene), Anti-NHE3 (Thermo Fisher), Anti-NHE7 (GeneTex, Irvine, CA), and Anti-NHE9 (Abcam, Cambridge, UK). Anti-MCT1 (Proteintech, Rosemont, IL), Anti-MCT2 (Bioss, Woburn, MA), Anti-MCT3 (Aviva Systems Biology), and Anti-MCT4 (Proteintech). Anti-V-Type ATPase (Clone H-5; Santa Cruz Biotochnology, CA).