Prilocaine cream

Mice were gonadectomized at 8-weeks of age. Anesthesia was achieved with isoflurane gas. In the female mice, bilateral ovariectomy was performed by making a 5 mm, longitudinal, dorsal incision through the skin and muscle wall. The ovary was separated from the uterus lateral to the oviduct using electrocautery, and the uterine horn was repositioned in the abdominal cavity. The muscle wall was closed using a 4–0 poliglecaprone suture (MWI Animal Health, Boise, ID, USA), and the skin was closed using a 4–0 nylon suture (MWI Animal Health, ID). The same procedure was then repeated on the contralateral ovary. In the male mice, bilateral gonadectomy was performed by making a single 2–3 mm, longitudinal, ventral incision through the scrotum. The testes were separated from the seminal vesicles with electrocautery. The skin was closed as described above. Incision sites were treated with a 2.5% lidocaine and 2.5% Prilocaine Cream (Akorn, Lake Forest, IL, USA) and Bacitracin Zinc antibiotic ointment (Trifecta Pharmaceuticals, Fort Lauderdale, FL, USA). Carprofen (0.05 mg/kg) was administered by subcutaneous injection immediately before the surgical procedure and 24-h post-operatively for analgesia.