Hrp conjugated goat anti human igg h l antibody

Manufactured by Jackson ImmunoResearch

Sourced in United States

HRP-conjugated goat anti-human IgG (H+L) antibody is a secondary antibody that binds to human immunoglobulin G (IgG) antibodies. It is conjugated with horseradish peroxidase (HRP) enzyme, which can be used for detection in various immunoassay techniques.

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Lab products found in correlation

Goat anti human igg fc antibody, by Jackson ImmunoResearch (1 mentions) He2 h5212, by ACROBiosystems (1 mentions) Protein g sepharose, by GE Healthcare (1 mentions) C digit blot scanner, by LI COR (1 mentions) Human serum, by Merck Group (1 mentions) Goat anti human fc specific antibody, by Merck Group (1 mentions) Yarra 3 μm sec 3000 column, by Phenomenex (1 mentions) Tetramethylbenzidine tmb, by LGC (1 mentions) Maxisorp 96 well plate, by Thermo Fisher Scientific (1 mentions) Elx800, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Goat anti-human IgG-HRP (33 citations) HRP-conjugated goat anti-human IgG (27 citations) Anti-human IgG-HRP (27 citations) HRP-conjugated anti-human IgG (18 citations) HRP-conjugated goat anti-human antibody (12 citations) HRP-conjugated goat anti-human IgG antibody (11 citations) Goat anti-human HRP IgG H+L (10 citations) HRP)-conjugated goat anti-human IgG (H+L) (6 citations) HRP-conjugated anti-human IgG antibody (5 citations) Anti-human IgG (H + L)/HRP (2 citations)

4 protocols using hrp conjugated goat anti human igg h l antibody

Pharmacokinetics of Anti-HER2 Antibodies

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Human FcRn transgenic mice (n = 3) were injected intraperitoneally with a single dose of 10 mg/kg human anti-HER2 IgG1, IgA or X-body. Blood was drawn from the tail vein at the indicated time points. Human IgG1 and X-body levels were measured using ELISA. Goat anti-human IgG Fc antibody (109-006-098, Jackson) and HRP-conjugated goat anti-human IgG (H+L) antibody (109-036-088, Jackson) were used for the capture and detection of human IgG1 and X-body, respectively. Human Her2 / ErbB2 Protein (HE2-H5212, Acrobiosystems) and Anti-6× His tag® antibody [AD1.1.10 (HRP) (ab178563, Abcam)] were used for the capture and detection of human IgA, respectively.

Liang Y., Li X., Peng F., Ye X., Wang W., Cen T., Li F., Lu Y., Liu Z., Liu H., Ding K., Ye K., Yu Y., Ma T., Zhang S., Huang Y., Wang Y., Yang X., Fu R, & Zhang H. (2022). Self-assembly of X-shaped antibody to combine the activity of IgG and IgA for enhanced tumor killing. Theranostics, 12(18), 7729-7744.

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Serum Stability of MOG-Seldeg-PS Protein

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MOG-Seldeg-PS was incubated in PBS at 4°C for 30 days or 37°C for 5 days, followed by analyses using a Phenomenex Yarra 3 μm SEC-3000 column (Phenomenex, 00H-4513-K0). For serum stability assays, endogenous IgGs were depleted from human serum (Sigma-Aldrich) by passage through protein G-Sepharose (GE Healthcare). MOG-Seldeg-PS was incubated in serum at a concentration of 400 nM at 37°C for 3 or 5 days as described previously.²⁶ (link) Following incubation, proteins were immunoprecipitated using agarose beads coupled with goat anti-human Fc-specific antibody (Sigma-Aldrich). Bound proteins were detected by immunoblotting with HRP-conjugated goat anti-human IgG (H+L) antibody (Jackson ImmunoResearch, West Grove, PA, USA). The immunoreactive bands were detected using WesternSure substrate (Thermo Fisher Scientific), followed by scanning with a C-DiGit blot scanner (LI-COR, Lincoln, NE, USA).

Sun W., Khare P., Wang X., Challa D.K., Greenberg B.M., Ober R.J, & Ward E.S. (2020). Selective Depletion of Antigen-Specific Antibodies for the Treatment of Demyelinating Disease. Molecular Therapy, 29(3), 1312-1323.

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ELISA Binding Assay for Antibody Characterization

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The IgG and Env glycoproteins (gp120 and gp140-F), which were produced in 293 F cells, were diluted in PBS (pH 7.4) at a concentration of 2 μg/ml and used to coat plates overnight at 4 °C. The plates were washed five times with 0.05% Tween 20 in PBS (PBS-T), blocked with 300 μl per well of blocking buffer (5% skim milk and 2% bovine albumin in PBS-T) for 1 hour at RT. 100 μl of each monoclonal antibody 5-fold serially diluted in blocking buffer was added and incubated for 1 hour at RT. Horseradish peroxidase (HRP)-conjugated goat anti-human IgG (H+L) antibody (Jackson ImmunoResearch Laboratories Inc., West Grove, PA) at 1:5,000 was added for 1 hour at RT. The plates were washed five times with PBS-Tween and then developed using 3,3´,5,5´-tetramethylbenzidine (TMB) (Kirkegaard & Perry Laboratories) at RT for 10 min. The reaction was stopped by the addition of 100 μl 1 N H₂SO₄ to each well. The readout was measured at a wavelength of 450 nm and all samples were analyzed in duplicate.

Dai K., Khan S.N., Wang Y., He L., Guenaga J., Ingale J., Sundling C., O’Dell S., McKee K., Phad G., Corcoran M., Wilson R., Mascola J.R., Zhu J., Li Y., Hedestam G.B, & Wyatt R.T. (2016). HIV-1 Vaccine-elicited Antibodies Reverted to Their Inferred Naive Germline Reveal Associations between Binding Affinity and in vivo Activation. Scientific Reports, 6, 20987.

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SARS-CoV-2 Spike Protein Antibody Assay

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Assays were performed in MaxiSorp 96-well plates (ThermoFisher) coated with 100 μL of recombinant SARS-CoV-2 WA1/2020 spike protein or bovine serum albumin diluted to 1 μg/mL in 1x PBS and incubated at 4°C overnight. Plates were then blocked with 10% FBS and 0.05% Tween 20 in 1x PBS (“blocking buffer”). Participant plasma was tested at 1:15 starting dilution followed by 7 or more 3-fold serial dilutions using blocking buffer as the diluent.
Plates were incubated for 90 minutes at 25°C and then washed 3 times with 1x PBS with 0.05% Tween 20. Secondary antibody (HRP-conjugated goat anti-human IgG (H+L) antibody, Jackson ImmunoResearch) was diluted 1:2500 in blocking buffer before adding it to the wells and incubating for 60 minutes at 25°C. Plates were washed 3 times with 1x PBS with 0.05% Tween 20, followed by 3 washes with 1x PBS before the addition of o-phenylenediamine dihydrochloride peroxidase substrate (MilliporeSigma). Reactions were stopped by the addition of 1 M HCl. Optical density measurements were taken at 490 nm using a microplate reader (ELx800, BioTek).

Cornfield D.N., Reeve H.L., Tolarova S., Weir E.K, & Archer S. (1996). Oxygen causes fetal pulmonary vasodilation through activation of a calcium-dependent potassium channel.. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 8089-8094.

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