Confocal laser scanning microscopy system

Manufactured by Nikon

The Confocal laser scanning microscopy system is a laboratory equipment that utilizes a focused laser beam to scan a sample and generate high-resolution, three-dimensional images. The system employs a pinhole aperture to filter out-of-focus light, resulting in improved contrast and optical sectioning capabilities compared to traditional microscopy techniques.

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Lab products found in correlation

Odyssey clx imaging system, by LI COR (2 mentions) Imaging station pearl impulse, by LI COR (2 mentions) Alexa fluor 546, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Anti oct4, by Merck Group (2 mentions) Anti nanog, by Cell Signaling Technology (2 mentions) Anti sox2, by Merck Group (2 mentions) Pkh26, by Merck Group (1 mentions) Dapi mountant, by Vector Laboratories (1 mentions)

Close spelling variants of this product

A1R confocal laser-scanning microscopy system Confocal laser scanning microscopy Laser confocal microscopy Confocal microscopy system Confocal microscopy

4 protocols using confocal laser scanning microscopy system

Tracking Fluorescently Labeled GDNP

Cited 1 time

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Six hours after oral administration of 2 × 10¹² /dose/mouse of either PKH26 or DiR fluorescent dye (Sigma) labelled GDNP, mice were sacrificed and the small intestine, colon, MLN, spleen and liver tissues were imaged. DiR fluorescent signal was detected and measured using the Imaging Station Pearl Impulse (Li-COR Biosciences). The labeled GDNP in the gut of mice were visualized using an Odyssey CLx Imaging System (Li-COR Biosciences). PKH26 signal in frozen tissue sections was observed using a confocal laser scanning microscopy system (Nikon, Melville, NY). The method has been described previously 19 (link).

Kumar A., Ren Y., Sundaram K., Mu J., Sriwastva M.K., Dryden G.W., Lei C., Zhang L., Yan J., Zhang X., Park J.W., Merchant M.L., Teng Y, & Zhang H.G. (2021). miR-375 prevents high-fat diet-induced insulin resistance and obesity by targeting the aryl hydrocarbon receptor and bacterial tryptophanase (tnaA) gene. Theranostics, 11(9), 4061-4077.

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Fluorescence Imaging of Nanoparticle Uptake

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After 6 h of orally administering 50 mg of either DiR or PKH26 fluorescent dye (Sigma) labelled GDNP, mice were sacrificed and the small intestine, colon, MLN, spleen and liver tissues were collected for imaging. DiR fluorescent signal was detected and measured using the Imaging Station Pearl Impulse (Li-COR Biosciences). The labeled GDNP in the gut of mice were visualized using an Odyssey CLx Imaging System (Li-COR Biosciences). PKH26 signal in frozen tissue sections was observed using a confocal laser scanning microscopy system (Nikon, Melville, NY). The method was previously described 14 (link). For fat uptake, nanoparticles were isolated from the high-fat diet-fed mice using our exosome isolation protocol 20 (link). Nanoparticles were labeled with DIR and orally administered to mice. After 6 h of oral administration, mice were sacrificed, the intestines harvested, flushed twice with PBS and scanned for DIR signals.

Kumar A., Sundaram K., Teng Y., Mu J., Sriwastva M.K., Zhang L., Hood J.L., Yan J., Zhang X., Park J.W., Merchant M.L, & Zhang H.G. (2022). Ginger nanoparticles mediated induction of Foxa2 prevents high-fat diet-induced insulin resistance. Theranostics, 12(3), 1388-1403.

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Immunocytochemistry of Stem Cell Markers

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Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% Triton X-100. Primary antibodies used were anti-OCT4 1:100 (Millipore), anti-SOX2 1:100 (Millipore) and anti-NANOG 1:100 (Cell Signalling). Secondary antibodies used were Alexa Fluor 488 and Alexa Fluor 546 (both at 1:500; Life Technologies). Cells were washed with 1 x PBS three times between incubations. Cells were mounted using Vectashield with DAPI mountant (Vector Laboratories) before being visualised on a confocal laser scanning microscopy system (Nikon).

Hepburn A.C., Steele R.E., Veeratterapillay R., Wilson L., Kounatidou E.E., Barnard A., Berry P., Cassidy J.R., Moad M., El-Sherif A., Gaughan L., Mills I.G., Robson C.N, & Heer R. (2019). The induction of core pluripotency master regulators in cancers defines poor clinical outcomes and treatment resistance. Oncogene, 38(22), 4412-4424.

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Immunofluorescent Staining of Pluripotency Markers

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Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% Triton X-100. Primary antibodies used were anti-OCT4 1:100 (Millipore), anti-SOX2 1:100 (Millipore) and anti-NANOG 1:100 (Cell Signaling). Secondary antibodies used were Alexa Fluor 488 and Alexa Fluor 546 (both at 1:500; Life Technologies). Cells were washed with 1 × PBS three times between incubations. Cells were mounted using Vectashield with DAPI mountant (Vector Laboratories) before being visualised on a confocal laser scanning microscopy system (Nikon).

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