Paraffin embedded tissue section protocol

Manufactured by Promega

The Paraffin-embedded tissue section protocol is a laboratory procedure used to prepare and analyze tissue samples embedded in paraffin wax. This protocol outlines the steps for deparaffinizing, rehydrating, and staining paraffin-embedded tissue sections for various analytical techniques.

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Lab products found in correlation

Lsm 700 confocal microscope, by Zeiss (2 mentions) Ab14196, by Abcam (1 mentions) Deadendtm fluorometric tunel system, by Promega (1 mentions)

2 protocols using paraffin embedded tissue section protocol

Apoptosis Detection in Testicular Tissue

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Apoptosis detection in the testes using the DeadEnd Fluorometric TUNEL System was carried out according to the standard paraffin-embedded tissue section protocol (Promega).
For annexin V staining of apoptotic cells, the sections were deparaffinized and rehydrated, followed by antigen retrieval in 10 mM of the sodium citrate buffer. Then, sections were blocked with goat serum in 0.3% Triton X-100 and incubated with primary antibodies of annexin V (Abcam, ab14196, 1:50).

Zhang Z.H., Jiang T.X., Chen L.B., Zhou W., Liu Y., Gao F, & Qiu X.B. (2020). Proteasome subunit α4s is essential for formation of spermatoproteasomes and histone degradation during meiotic DNA repair in spermatocytes. The Journal of Biological Chemistry, 296, 100130.

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Apoptotic DNA Fragmentation Analysis

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Apoptotic DNA fragmentation was analyzed using the DeadEnd^TM Fluorometric TUNEL System according to the standard paraffin-embedded tissue section protocol (Promega). The DNAs in nuclei were stained with DAPI at the final preparation step. The slides were viewed on a Zeiss LSM 700 confocal microscope using a ×100 oil objective and laser at 488, 555, or 639 nm for excitation.

Liu S.S., Jiang T.X., Bu F., Zhao J.L., Wang G.F., Yang G.H., Kong J.Y., Qie Y.F., Wen P., Fan L.B., Li N.N., Gao N, & Qiu X.B. (2024). Molecular mechanisms underlying the BIRC6-mediated regulation of apoptosis and autophagy. Nature Communications, 15, 891.

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