Westernbright ecl solution

Manufactured by Advansta

Sourced in United States, Israel

WesternBright ECL solution is a chemiluminescent substrate used for the detection of proteins in Western blot analysis. It is designed to provide a sensitive and reliable method for visualizing protein bands on membranes.

Automatically generated - may contain errors

Lab products found in correlation

C digit blot scanner, by LI COR (1 mentions) Semi dry transfer machine, by Bio-Rad (1 mentions) Nupage tris acetate gel, by Thermo Fisher Scientific (1 mentions) Anti g6pdh, by Merck Group (1 mentions) Anti ha hrp 3f10, by Roche (1 mentions) Anti erα, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Cyclin d1, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

WesternBright Sirius ECL solution WesternBright ECL kit solution WesternBright ECL Luminol/enhancer solution WesternBright ECL ECL solution

4 protocols using westernbright ecl solution

Histone Acetylation Profiling in Neuro2a Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neuro2a cells were treated with different
concentrations of compounds and vehicle. The cells incubated with
different treatments were washed with 1× PBS and harvested in
1× laemmli sample buffer. The cell lysate total protein relative
concentration estimated with amido black method and an equal amount
of protein was loaded onto 12% sodium dodecyl sulfate-polyacrylamide
gel electrophoresis gel. Blocking was done for 1 h at room temperature,
followed by incubation with primary antibodies H3 acetyl (1:1000),
H4 acetyl (1:2000), and H3 PAN (1:3000) overnight at 4 °C. The
blots were washed with 1× PBST, and incubation in secondary antibody
anti-rabbit (1:5000) was done at room temperature for 1 h. The blots
were again washed with 1× PBST and then developed with the Bio-Rad
ChemiDoc instrument using WesternBright ECL solution (advansta). The
band intensities of Western blots were measured using ImageJ software.

Reddy R.G., Dachavaram S.S., Reddy B.R., Kalyankar K.B., Rajan W.D., Kootar S., Kumar A., Das S, & Chakravarty S. (2018). Fellutamide B Synthetic Path Intermediates with in Vitro Neuroactive Function Shows Mood-Elevating Effect in Stress-Induced Zebrafish Model. ACS Omega, 3(9), 10534-10544.

+ Open protocol

+ Expand

Exosome and Cell Lysate Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total cell lysates for colon cancer cells (10 µg) and exosomes (10 µg) were separated on 12% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) at 120 V for 90 min and transferred to nitrocellulose membranes. After blocking in 5% non-fat milk in tris-buffered saline with tween 20 (TBST) for 2 h, the membranes were blotted with primary antibodies at 4 °C overnight for 16 h, followed by secondary bodies at a dilution of 1:2000. The protein bands were visualized using WesternBright ECL solution (Advansta, San Jose, CA, USA) and the signals were captured using a C-digit blot scanner (LI-COR). Primary antibodies against KRAS, HSC70, CD63, CD9 and CD81, and secondary antibodies (mouse anti-rabbit and goat anti-mouse) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Primary antibodies against actin and p53 were purchased from Millipore (Burlington, VT, USA) while primary antibodies against ACSL4 were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Western blotting was performed in triplicate for each tested protein.

Eng S.K., Imtiaz I.R., Goh B.H., Ming L.C., Lim Y.C, & Lee W.L. (2021). Does KRAS Play a Role in the Regulation of Colon Cancer Cells-Derived Exosomes?. Biology, 10(1), 58.

+ Open protocol

+ Expand

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted by the TCA (Trichloroacetic acid) method if not stated otherwise. Samples were loaded into 7.5% or 4–15% gradient Tris-Glycine BioRad stain-free pre-casted gels for routine analysis. Samples from pull-downs analyses were loaded into 3–8% gradient NuPAGE Tris-Acetate gels (Invitrogen). Gels were transferred to PVDF membranes using a semi-dry transfer machine (BioRad) and hybridized on TBST 5% milk with the appropriate antibodies. Antibodies were used at the suggested dilution: anti-HA-HRP (3F10, Roche) 1:1000 (1:500 for pull-down analysis), anti-Ubiquitin (P4D1, Biolegends) 1:1000, anti-Smt3 (provided by B.Palancade) 1:2000, anti-G6PDH (A9521, Sigma) 1:20,000, anti-Pgk1-HRP (22C5D8, Abcam) 1:10,000, anti-GST-HRP (GERPN1236, Sigma) 1:5000. When required, HRP-conjugated secondary antibodies from Cell Signaling (anti-Mouse-HRP, anti-Rabbit-HRP) were used at 1:10,000 dilution. Western blots were revealed with WesternBright ECL solution (Advansta) or WesternBright Sirius HRP subsrate if required (Advansta). Blots were cropped in order to arrange figures without any lane substitution and conserving the area with immunoblotting signal (examples of uncropped images are available in Supplementary Figure 9).

Talhaoui I., Bernal M., Mullen J.R., Dorison H., Palancade B., Brill S.J, & Mazón G. (2018). Slx5-Slx8 ubiquitin ligase targets active pools of the Yen1 nuclease to limit crossover formation. Nature Communications, 9, 5016.

+ Open protocol

+ Expand

Genistein Induces Protein Expression Changes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following the exposure of MCF-7 and differentiating 3T3-L1 cells to genistein, each group of cells was subjected to lysis in radio-immunoprecipitation assay (RIPA) buffer [1% nonyl phenoxypolyethoxylethanol (NP)-40, 150 mM NaCl, 0.05% deoxycholic acid (DOC), 1% sodium dodecyl sulfate (SDS) and 50 mM tris(hydroxymethyl)aminomethane (Tris), pH 7.5] containing protease inhibitors for 1 h at 4°C. The supernatant was separated by centrifugation and the protein concentration was determined using a Bradford protein assay kit 2 (Bio-Rad). The proteins were then transferred to nitrocellulose membranes (0.45 μm). The membranes were blocked with 1% bovine serum albumin (BSA) for 1.5 h, washed twice with phosphate-buffered saline (PBS) containing 0.2% Tween-20, and incubated with the respective primary antibodies [cyclin D1, anti-ERα, -B-cell lymphoma 2 (-Bcl-2), -Bcl-2-associated X protein (-Bax) and -β-actin; Santa Cruz Biotechnology, Inc.] overnight at 4°C. The next day, the immunoreaction was continued using secondary rabbit anti-rabbit horseradish-peroxidase-conjugated antibodies following washing for 2 h at room temperature. Bands were detected with MicroChemi (DNR Bio-Imaging System, Ltd., Jerusalem, Israel) using WesternBright^™ ECL solution (Advansta Inc., Menlo Park, CA, USA).

CHOI E.J., JUNG J.Y, & KIM G.H. (2014). Genistein inhibits the proliferation and differentiation of MCF-7 and 3T3-L1 cells via the regulation of ERα expression and induction of apoptosis. Experimental and Therapeutic Medicine, 8(2), 454-458.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!