Protein samples were separated on SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). PVDF membranes were incubated with primary antibody, then incubated with horseradish peroxidase conjugated secondary antibody. After washing 3 times for 10 min in TBST, the proteins were visualized with an ECL detection system. ImageJ software (BD, Franklin Lakes, NJ, USA) was used to quantify the level of protein expression by calculating integrated density value (IDV) using GAPDH as a control. Antibodies against ABCB1, ABCC1, ABCC3, EGFR, or IL-6 were purchased from Cell Signaling Technology (Danvers, MA, USA).
Abcc3
Manufactured by Cell Signaling Technology
Sourced in United States
ABCC3 is a membrane-bound protein that functions as an ATP-binding cassette (ABC) transporter. It plays a role in the transport of various organic anions across cell membranes. The core function of ABCC3 is to facilitate the movement of substrates in and out of cells.
Automatically generated - may contain errors
Lab products found in correlation
Imagej software, by BD (1 mentions)
Abcc1, by Cell Signaling Technology (1 mentions)
Interleukin 6 (il 6), by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Miquelianin, by Merck Group (1 mentions)
Fluorescent mounting medium, by Agilent Technologies (1 mentions)
Phosphate buffered saline pbs, by Biosesang (1 mentions)
Anti nrf2, by Cell Signaling Technology (1 mentions)
Minimum essential medium (mem), by Welgene (1 mentions)
Abcc2, by Cell Signaling Technology (1 mentions)
Alexa 488 conjugated anti rabbit secondary antibodies, by Cell Signaling Technology (1 mentions)
Cyp1a1, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Quercetin, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Isorhamnetin, by Merck Group (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
Trypsin edta, by Welgene (1 mentions)
2 protocols using abcc3
1
Protein Expression Analysis by Western Blot
2
Molecular Mechanisms of Phytochemical-Mediated AhR Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Quercetin, isorhamnetin, miquelianin, B[a]P, 4,6-diamidino-2-phenylindole dihydrochloride (DAPI), nuclease P1, dimethyl sulfoxide (DMSO), and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Minimum essential medium (MEM), penicillin/streptomycin, fetal bovine serum (FBS), sodium pyruvate, and trypsin-EDTA were purchased from Welgene (Daegu, South Korea). Phosphate-buffered saline (PBS) was purchased from Biosesang (Seongnam, South Korea). Fluorescent mounting medium was purchased from Dako (Santa Clara, CA, USA). Antibodies (anti-NRF2, AhR, CYP1A1, GSTA1, CYP1A1, CYP1B1, ABCC2, ABCC3, and β-actin) and Alexa 488-conjugated anti-rabbit secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
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