Fidaxomicin

Manufactured by Selleck Chemicals

Sourced in United States

Fidaxomicin is a macrocyclic antibiotic compound used in laboratory settings. It functions as an inhibitor of bacterial RNA polymerase.

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Lab products found in correlation

4 protocols using fidaxomicin

Fluorescent Transcription and Antibiotic Quantification

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All transcription reactions were performed with NTPs (Thermo Scientific), 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) dye (Sigma Aldrich; catalogue # SML1627), and RiboLock RNase inhibitors (Thermo Scientific; catalogue # EO0381). DFHBI concentration was determined using an extinction coefficient at 420 nm of 31,611 M⁻¹ cm⁻¹. Rifampicin (Sigma-Aldrich; catalogue #R3501) and Fidaxomicin (Selleckchem; catalogue # S4227) solids were dissolved in DMSO and molar concentrations were determined by weight (Fidaxomicin) or by absorbance using an extinction coefficient at 470 nm of 15,300 M⁻¹ cm⁻¹ (48 ) (Rifampicin).

Jensen D., Ruiz Manzano A., Rector M., Tomko E.J., Record M.T, & Galburt E.A. (2023). High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for the Mycobacterium tuberculosis RNA polymerase. Nucleic Acids Research, 51(19), e99.

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Antibiotic Compounds Procurement Protocol

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Ciprofloxacin hydrochloride was purchased from MP Biomedicals (Santa Ana, CA, USA); erythromycin hydrochloride, vancomycin hydrochloride, ampicillin, ceftriaxone sodium, levofloxacin, imipenem monohydrate, gentamicin sulfate, tetracycline hydrochloride, colistin sulfate, penicillin G potassium, teicoplanin, linezolid, clindamycin hydrochloride, and metronidazole were purchased from Sigma-Aldrich (St Louis, MO, USA); meropenem was purchased from TCI (Portland, OR, USA); and daptomycin and fidaxomicin were purchased from Selleckchem (Houston, TX, USA).

D’Atanasio N., Capezzone de Joannon A., Di Sante L., Mangano G., Ombrato R., Vitiello M., Bartella C., Magarò G., Prati F., Milanese C., Vignaroli C., Di Giorgio F.P, & Tongiani S. (2020). Antibacterial activity of novel dual bacterial DNA type II topoisomerase inhibitors. PLoS ONE, 15(2), e0228509.

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Fluorescent RNA Labeling and Inhibition Assay

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All transcription reactions were performed with NTPs (Thermo Scientific; catalog #s: ATP – R0441, CTP – R0451, GTP – R0461, and UTP – R0471), 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) dye (Sigma Aldrich; catalog # SML1627), and RiboLock RNase inhibitors (Thermo Scientific; catalog # EO0381). DFHBI concentration, stored in 100 mM HEPES, pH 7.6, was determined by absorbance using an extinction coefficient at 420 nm of 31,611 M⁻¹cm⁻¹. Salmon-sperm DNA (Invitrogen; catalog # 15632011) was used as a competitor for single-round experiments (17 (link)). Rifampicin (Sigma-Aldrich; catalog # R3501) and Fidaxomicin (Selleckchem; catalog # S4227) solids were dissolved in DMSO. The molar concentration of Fidaxomicin was determined by weight and of Rifampicin by absorbance using an extinction coefficient at 470 nm of 15,300 M⁻¹cm⁻¹ (88 ).

Jensen D., Manzano A.R., Rector M., Tomko E.J., Record M.T, & Galburt E.A. (2023). High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for Mycobacterium tuberculosis RNA polymerase. bioRxiv.

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In Vivo Murine Clostridium Infection Protocol

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CBM 588 bacterial powder was used for all in vivo studies at a concentration of 2.2*10¹⁰ cfu/g (Lot 61GT: MIYARISAN Pharmaceutical Co. Ltd., Tokyo, Japan). Clindamycin for injection was purchased from Pfizer Japan Inc (Tokyo, Japan). Analytical grade fidaxomicin was purchased from Selleck Chemicals Inc (Houston, TX, USA). and diluted with dimethyl sulfoxide (FUJIFILM Wako Chemical Co. Ltd., Osaka, Japan) with 500 mg/mL used as the stock solution. Immediately before each in vivo experiment, CBM 588 powder was weighed and reconstituted with sterile water. Clindamycin and fidaxomicin were further diluted to achieve the desired concentration with distilled water. fidaxomicin solution was stored 4ºC and discarded 12 h after reconstitution. Neutralizing antibody to IL-17A was generated at BD Biosciences (Franklin Lakes, NJ, USA).

Hagihara M., Ariyoshi T., Kuroki Y., Eguchi S., Higashi S., Mori T., Nonogaki T., Iwasaki K., Yamashita M., Asai N., Koizumi Y., Oka K., Takahashi M., Yamagishi Y, & Mikamo H. (2021). Clostridium butyricum enhances colonization resistance against Clostridioides difficile by metabolic and immune modulation. Scientific Reports, 11, 15007.

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