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Goat anti mouse igg conjugated with fitc

Manufactured by Proteintech
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Goat anti-mouse IgG conjugated with FITC is a secondary antibody that binds to mouse primary antibodies. The FITC (fluorescein isothiocyanate) label allows for the detection of bound primary antibodies using fluorescence microscopy or flow cytometry.

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Lab products found in correlation

Cy3 rabbit and fitc mouse conjugated igg, by Proteintech (2 mentions)

Spelling variants of this product

FITC-conjugated goat anti-mouse IgG (22 citations) FITC goat anti-mouse IgG (6 citations) Anti-mouse conjugated FITC (4 citations) Goat anti-mouse FITC (2 citations) FITC anti-mouse IgG (2 citations) Anti-TM (2 citations)

2 protocols using goat anti mouse igg conjugated with fitc

1

Visualizing HBx and TRIM5γ Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
HepG2 cells were transfected with Flag-HBx plasmids or together with HA-TRIM5γ plasmids; 48 hours later, cells were fixed in acetone-methanol (1:1) at 37°C for 10 min. Subsequently, cells were washed with PBS and blocked with 5% BSA in PBST for 1 h, incubated with Flag(Mouse) and HA(Rabbit) antibody at 37°C for 1 h, washed in PBS, and then incubated with goat anti-mouse IgG conjugated with FITC or both Cy3(rabbit) and FITC(mouse) conjugated IgG (Proteintech). Cells were washed with PBS and observed under a fluorescence microscope.
Tan G., Yi Z., Song H., Xu F., Li F., Aliyari R., Zhang H., Du P., Ding Y., Niu J., Wang X., Su L., Qin F.X, & Cheng G. (2019). Type-I-IFN-Stimulated Gene TRIM5γ Inhibits HBV Replication by Promoting HBx Degradation. Cell reports, 29(11), 3551-3563.e3.
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2

Visualizing HBx and TRIM5γ Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
HepG2 cells were transfected with Flag-HBx plasmids or together with HA-TRIM5γ plasmids; 48 hours later, cells were fixed in acetone-methanol (1:1) at 37°C for 10 min. Subsequently, cells were washed with PBS and blocked with 5% BSA in PBST for 1 h, incubated with Flag(Mouse) and HA(Rabbit) antibody at 37°C for 1 h, washed in PBS, and then incubated with goat anti-mouse IgG conjugated with FITC or both Cy3(rabbit) and FITC(mouse) conjugated IgG (Proteintech). Cells were washed with PBS and observed under a fluorescence microscope.
Tan G., Yi Z., Song H., Xu F., Li F., Aliyari R., Zhang H., Du P., Ding Y., Niu J., Wang X., Su L., Qin F.X, & Cheng G. (2019). Type-I-IFN-Stimulated Gene TRIM5γ Inhibits HBV Replication by Promoting HBx Degradation. Cell reports, 29(11), 3551-3563.e3.
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