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Truseq stranded total rna globin kit

Manufactured by Illumina
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The TruSeq Stranded Total RNA—Globin kit is a laboratory equipment product designed for targeted depletion of globin transcripts from total RNA samples. It is used to improve the detection and quantification of low-abundance transcripts in RNA-sequencing experiments.

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Lab products found in correlation

Novaseq 6000, by Thermo Fisher Scientific (2 mentions) Fragment analyzer, by Agilent Technologies (2 mentions) Fragment analyzer, by Thermo Fisher Scientific (2 mentions) Qubit 3.0 fluorometer, by Thermo Fisher Scientific (2 mentions)

2 protocols using truseq stranded total rna globin kit

1

RNA Sequencing Protocol with NovaSeq 6000

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was assessed with a Fragment Analyzer (Agilent) using the Standard Sensitivity RNA kit. Total RNA was normalized to 100 ng prior to random hexamer priming and libraries generated using the TruSeq Stranded Total RNA—Globin kit (Illumina, San Diego, USA). Libraries were assessed on the Fragment Analyzer using the High Sense Large Fragment kit and quantified using a Qubit 3.0 fluorometer (Life Technologies). Sequencing was performed on a NovaSeq 6000 (Thermo Fisher) using a 50 base-pair, paired-end run for a minimum of 30 million reads per sample.
Francis M.E., Richardson B., Goncin U., McNeil M., Rioux M., Foley M.K., Ge A., Pechous R.D., Kindrachuk J., Cameron C.M., Richardson C., Lew J., Machtaler S., Cameron M.J., Gerdts V., Falzarano D, & Kelvin A.A. (2021). Sex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets. Scientific Reports, 11, 14536.
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2

Transcriptome Analysis via RNA-Seq

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was assessed with a Fragment Analyzer (Agilent) using the Standard Sensitivity RNA kit. Total RNA was normalized to 100 ng prior to random hexamer priming and libraries generated using the TruSeq Stranded Total RNA – Globin kit (Illumina, San Diego, USA). Libraries were assessed on the Fragment Analyzer using the High Sense Large Fragment kit and quantified using a Qubit 3.0 fluorometer (Life Technologies). Sequencing was performed on a NovaSeq 6000 (Thermo Fisher) using a 50 base-pair, paired-end run for a minimum of 30 million reads per sample.
Francis M.E., Richardson B., McNeil M., Rioux M., Foley M.K., Ge A., Pechous R.D., Kindrachuk J., Cameron C.M., Richardson C., Lew J., Cameron M.J., Gerdts V., Falzarano D, & Kelvin A.A. (2021). Male sex and age biases viral burden, viral shedding, and type 1 and 2 interferon responses during SARS-CoV-2 infection in ferrets. bioRxiv.
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