Wogonoside

Manufactured by Merck Group

Sourced in United States

Wogonoside is a laboratory chemical compound produced by Merck Group. It is a flavonoid glycoside that can be used as a reference standard or analytical reagent in research and development applications.

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Lab products found in correlation

Wogonin, by Merck Group (5 mentions) Baicalin, by Merck Group (3 mentions) Baicalein, by Merck Group (2 mentions) Zorbax sb c18 column, by Agilent Technologies (1 mentions) Ag11132, by Coriell Institute for Medical Research (1 mentions) Nor wogonin, by ChemFaces (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 10a, by American Type Culture Collection (1 mentions) Hcc1806, by American Type Culture Collection (1 mentions) Bt549, by American Type Culture Collection (1 mentions) Hcc70, by American Type Culture Collection (1 mentions) 4 nitrophenyl β d glucopyranoside pnpg, by Merck Group (1 mentions) F344 rats, by Charles River Laboratories (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Annexin 5 apoptosis detection kit, by BD (1 mentions) Hyclone, by Cytiva (1 mentions) Scutellarein, by Merck Group (1 mentions) Verbascoside, by ChromaDex (1 mentions) Chrysin, by Merck Group (1 mentions)

7 protocols using wogonoside

HPLC Analysis of Flavonoids in Herbal Samples

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High-performance liquid chromatography (HPLC) was used for the analytical determination of flavonoids in SB. Samples and standards were analyzed using a Waters 600 system coupled to Waters 2487 UV dual wavelengths absorbance detector (Waters Chromatography Canada Inc.), and Empower PDA software (Milford, MA USA). An Agilent Zorbax SB-C18 column (4.6 × 250 mm, 5 μm) was applied for this analysis. Sample preparation and extraction of flavonoids were done using a previously described method with modifications [15 (link)]. Before extraction, the herbal powder was soaked in Britton-Robinson buffer, pH 6.5 at 50 °C for 30 min. Flavone standards, baicalin (99%), baicalein (98%), wogonoside (≥95%), wogonin (≥98%) were purchased from Sigma-Aldrich. All HPLC-grade solvents were filtered through a membrane filter (0.2 μm pore size). The content of the constituents was calculated using standard curves acquired for four flavonoids. All measurements were performed in triplicate.

Króliczewska B., Graczyk S., Króliczewski J., Pliszczak-Król A., Miśta D, & Zawadzki W. (2017). Investigation of the immune effects of Scutellaria baicalensis on blood leukocytes and selected organs of the chicken’s lymphatic system. Journal of Animal Science and Biotechnology, 8, 22.

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Evaluating Anti-TNBC Compounds in Cell Lines

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Human TNBC cell lines (MDA-MB-231, BT-549, HCC70, and HCC1806) and non-tumorigenic breast cell line (MCF-10A) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). A normal breast cell line (AG11132) was obtained from Coriell Institute for Medical Research (Camden, NJ, USA). TNBC cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium while non-tumorigenic breast cells (MCF-10A and AG11132) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) or Mammary Epithelial Cell Growth Medium (MEGM), respectively. The media contained 10 % fetal calf serum (FCS) and were cultured in a humidified atmosphere with 5 % CO₂ at 37 °C. Cells between the 3^rd and 10^th passages were used for this study. Nor-wogonin was purchased from Chem Faces (Wuhan, Hubei, China) whereas wogonin and wogonoside were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Abd El-Hafeez A.A., Khalifa H.O., Mahdy E.A., Sharma V., Hosoi T., Ghosh P., Ozawa K., Montano M.M., Fujimura T., Ibrahim A.R., Abdelhamid M.A., Pack S.P., Shouman S.A, & Kawamoto S. (2019). Anticancer effect of nor-wogonin (5, 7, 8-trihydroxyflavone) on human triple-negative breast cancer cells via downregulation of TAK1, NF-κB, and STAT3. Pharmacological reports : PR, 71(2), 289-298.

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Wogonoside and Wogonin Glucosidation Assay

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Wogonoside, wogonin, and 4-Nitrophenyl β-d-glucopyranoside (pNPG) (purity > 98) were purchased from Sigma Aldrich (St. Louis, MO, USA). Monopotassium phosphate (KH2PO4), dipotassium phosphate (K2HPO4), and sodium chloride (NaCl) were purchased from VWR International (Radnor, PA, USA). KPI buffer was made using KH2PO4 and K2HPO4 and the pH was adjusted using sodium hydroxide and hydrochloride. Other chemicals (typically analytical grade or better) were used as received. C57BL6 mice (male, 8 weeks) were obtained from Jackson lab (Bar Harbor, ME, USA) and F344 rats (male, 8 weeks) were bought from Charles River (Wilmington, MA, USA).

Ebuzoeme C., Etim I., Ikimi A., Song J., Du T., Hu M., Liang D, & Gao S. (2021). Glucuronides Hydrolysis by Intestinal Microbial β-Glucuronidases (GUS) Is Affected by Sampling, Enzyme Preparation, Buffer pH, and Species. Pharmaceutics, 13(7), 1043.

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Wogonoside Modulates ER Stress Pathways

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Wogonoside was purchased from Sigma-Aldrich (Merck KGaA) and dissolved in phosphate-buffered saline (PBS; HyClone; Cytiva). The Cell Counting Kit (CCK)-8 Kit assay was purchased from Dojindo Molecular Technologies, Inc. The primary antibodies targeting IRE1α (cat. no. 3294; rabbit monoclonal), tumor necrosis factor receptor-associated factor 2 (TRAF2; cat. no. 4724; rabbit polyclonal), mitogen-activated protein kinase kinase kinase 5 (ASK; cat. no. 3762; rabbit polyclonal), phosphorylated(p)-ASK (cat. no. 3764; rabbit polyclonal), JNK (cat. no. 4668; rabbit monoclonal), JNK (cat. no. 9252; rabbit polyclonal), DNA damage-inducible transcript 3 protein (CHOP; cat. no. 2895; mouse monoclonal), endoplasmic reticulum chaperone BiP [glucose regulated protein (GRP78); cat. no. 3177; rabbit monoclonal], endoplasmin (GRP94; cat. no. 20292; rabbit monoclonal), cleaved capsase-3 (cat. no. 9664; rabbit monoclonal), capsase-3 (cat. no. 9662; rabbit monoclonal), cleaved capsase-9 (cat. no. 9505; rabbit monoclonal), capsase-9 (cat. no. 9502; rabbit monoclonal), Bcl-2 (cat. no. 4223; rabbit monoclonal), BAX (cat. no. 5023; rabbit monoclonal) and GAPDH (cat. no. 5174; rabbit monoclonal) were purchased from Cell Signaling Technology, Inc. Distilled water was used in all experiments for washing the cells. The Annexin V Apoptosis Detection kit was obtained from BD Biosciences.

Gu Q., Zhu C., Wu X., Peng L., Huang G, & Hu R. (2021). Wogonoside promotes apoptosis and ER stress in human gastric cancer cells by regulating the IRE1α pathway. Experimental and Therapeutic Medicine, 21(4), 411.

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Huang Qin Ge Gen Tang Herb Extraction

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Herb granules [(HQ, Scutellaria baicalensis Georgi Radix), Gegen (GG, Pueraria lobata Ohwi Radix), Shengma (SM, Cimicifuga foetida L. Rhizoma), Baishao (BS, Paeonia lactiflora Pall Radix) and Gancao (GC, Glycyrrhiza uralensis Fisch Radix)] were purchased from Sun Ten Pharmaceutical Co. (Taipei, Taiwan). The herb weight ratio for Huang Qin Ge Gen Tang formulation (HQGGT) was HQ:10 g; GG:15 g; SM:6 g; BS:10 g; GC:3 g as previously described [12 (link)]. Granules were weighed and resuspended in deionized water and incubated at 80 °C for 30 min. The supernatant was separated from any insoluble material by centrifugation (3000 × g, 20 min) and sterilized by passage through a 0.22 μm filter. For in vitro cell culture studies, 100 mg/mL HQ extract was prepared. For in vivo animal experiments, 300 mg/mL HQ (actual soluble weight is 200 mg/mL) and 20 mg/mL BI solution (DMSO-PBS solution) were used. Baicalin (BI), baicalein (BE), wogonin (WO), and wogonoside (WS) (Sigma; St. Louis, MO) were dissolved in DMSO (50 mM stock solution).

Liu H., Liu H., Zhou Z., Chung J., Zhang G., Chang J., Parise R.A., Chu E, & Schmitz J.C. (2023). Scutellaria baicalensis enhances 5-fluorouracil-based chemotherapy via inhibition of proliferative signaling pathways. Cell Communication and Signaling : CCS, 21, 147.

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Profiling Phytochemical Composition of Skullcap

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The dried and pulverised biomass samples were extracted with 50 mL of analytical-grade methanol for 2 h at 78 ± 2 °C under a reflux condenser. After extraction, the obtained extracts were filtered through a paper filter, transferred to crystallisers, and left at room temperature to allow the solvent to evaporate. The remains were dissolved in 4 mL of HPLC-grade methanol (Merck) and subjected to HPLC. Qualitative and quantitative HPLC with diode array detection (DAD) was performed as described previously [20 (link),66 (link)]. Compounds were identified based on their retention times and UV spectra, as well as by comparison with reference substances (27 flavonoids, 19 phenolic acids, benzoic and cinnamic acids, 2 phenylethanoid glycosides). The reference standards used for the HPLC analyses of skullcap-specific flavonoids were baicalein, baicalin, scutellarin, wogonin (ChromaDex, Irvine, CA, USA), chrysin, scutellarein, wogonoside (Sigma–Aldrich Co., Saint Louis, MO, USA), oroxylin A and skullcapflavone II (ChemFaces Biochemical Co., Wuhan, China). The verbascoside was purchased form ChromaDex, Irvine, CA, USA.

Kwiecień I., Miceli N., D’Arrigo M., Marino A, & Ekiert H. (2022). Antioxidant Potential and Enhancement of Bioactive Metabolite Production in In Vitro Cultures of Scutellaria lateriflora L. by Biotechnological Methods. Molecules, 27(3), 1140.

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Baicalein and Wogonin: Novel Therapeutic Compounds

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Baicalein (purity 98%), baicalin (purity 95%), wogonin (purity > 98%), wogonoside (purity > 95%), and tunicamycin were obtained from Sigma-Aldrich (St. Louis, MO). Cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) and Fluo-3 AM were from Beyotime Institute of Biotechnology (Nantong, China). Antiphospho-PERK (Thr-981) rabbit polyclonal antibody (sc-32577) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Other antibodies were obtained from Cell Signaling Technology (Beverly, MA).

Wang Z., Jiang C., Chen W., Zhang G., Luo D., Cao Y., Wu J., Ding Y, & Liu B. (2014). Baicalein Induces Apoptosis and Autophagy via Endoplasmic Reticulum Stress in Hepatocellular Carcinoma Cells. BioMed Research International, 2014, 732516.

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