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2 protocols using 3 methyl 2 butenal

1

DNPH-based Carbonyl Compound Analysis

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2,4-Dinitrophenylhydrazine (DNPH) was purchased from Tokyo Chemical Industry (TCI, Portland, OR); d3-labeled DNPH (>98% isotopic purity) and malondialdehyde (MDA) was obtained from Santa Cruz Biotechnology (Dallas, TX); Ketones Mix (Propanone, 2-Butanone, 4-Methyl-2-Pentanone, 2-Hexanone, Cyclohexanone) and TO11/IP-6A Aldehyde/Ketone-DNPH Mix (Acetaldehyde-DNPH, Propanone-DNPH, Propanal-DNPH, Acrolein-DNPH, Benzaldehyde-DNPH, Butanal-DNPH, Crotonaldehyde-DNPH, 2,5-Dimethylbenzaldehyde-DNPH, Formaldehyde-DNPH, Hexanal-DNPH, Pentanal-DNPH, Isopentanal-DNPH, o-Tolualdehyde-DNPH, m-Tolualdehyde-DNPH, p-Tolualdehyde-DNPH), 4-hydroxy-3-methoxybenzaldehyde (Vanillin), Indole-3-carboxaldehyde, Hexanophenone, Heptanophenone, 2,3-Butanedione, 2,3-Pentanedione, trans-2-methyl-2-butenal, 3-methyl-2-butenal (methylcrotonaldehyde), sodium acetate, and concentrated HCl were obtained from Sigma Aldrich (Milwaukee, WI); 13C2-Acetaldehyde was obtained from Cambridge Isotope Laboratories (Tewksbury, MA); Methylglyoxal (MG) was obtained from Thermo Fisher (Morris Plains, NJ); Strata-X polymeric reverse-phase cartridge was purchased from Phenomenex (Torrance, CA). All acids and organic solvents were HPLC grade or better.
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2

Analytical Standards for Flavour Profiling

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Eucalyptol (1,8-cineole) (Fluka), phenylethyl alcohol (Fluka), heptanal, (R)-(+)-β-citronellol, linalool, (-)-carvone, octanal, nonanal, trans-2-octenal, trans-2-nonenal, trans-2-decenal, 3-methyl-2-butenal, 2-octanone, 2tridecanone, propanoic acid, butanoic acid, indole, terpineol (mixture of isomers), and tetradecanoic acid analytical standards were purchased from Sigma-Aldrich (Pty) Ltd. Kempton Park, South Africa. NLEA FAME mixture, containing hexadecanoic acid, methyl ester, was purchased from Restek (Bellefonte, PA, USA).
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