Steady-state absorption spectra (1 nm resolution) were obtained using an Agilent Cary 60 spectrophotometer (Agilent Technologies, USA) with a 1 cm pathlength quartz cuvette. Measurements were taken from 350 nm to 600 nm in 1 nm steps at a concentration of 5 μM. Emission spectra were obtained at 1 μM with a spectrofluorometer (FluoTime 300, Picoquant, Berlin, Germany). The emission spectra were measured between 450–650 nm with a 1 nm step after 440 ± 5 nm excitation.
Agilent cary 60 spectrophotometer
Manufactured by Agilent Technologies
Sourced in United States
The Agilent Cary 60 spectrophotometer is a versatile and efficient instrument designed for accurate and reliable absorbance measurements. It is capable of performing a wide range of spectroscopic analyses across various applications.
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Lab products found in correlation
Fluotime 300, by PicoQuant (2 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Zetasizer nano, by Malvern Panalytical (1 mentions)
Fei tecnai g2 f20, by Thermo Fisher Scientific (1 mentions)
Vertex 70, by Bruker (1 mentions)
1 4 dioxane, by Reanal (1 mentions)
Avance drx 400, by Bruker (1 mentions)
High performance liquid chromatography (hplc), by Avantor (1 mentions)
Nylon filter, by Merck Group (1 mentions)
9 protocols using agilent cary 60 spectrophotometer
1
Steady-state Absorption and Emission Spectra
2
Absorption and Emission Spectroscopy
3
Multimodal Characterization of Nanoparticles
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TEM images were acquired on a FEI Tecnai G2 F-20 transmission electron microscope (FEI Company, Hillsboro, OR, USA) with an accelerating voltage of 200 kV. DLS measurements were performed on a Nano Zetasizer (Malvern Panalytical Ltd., Malvern, UK). The ultraviolet-visible spectra were recorded on an Agilent Cary 60 spectrophotometer (Agilent Technologies, Santa Clara, USA). XPS analysis was performed on an ESCALAB 250Xi x-ray photoelectron spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). EDS and element mapping images were acquired using a FEI Titan G2 F-30 instrument operated at 200 kV. The Fe and Cu were determined by ICP-OES analysis using a Flexar/NexlON300X inductively coupled plasma optical emission spectrometer (PerkinElmer Inc., Waltham, MA, USA).
4
Isolation and Characterization of E. coli Cytochromes
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The cytochromes bd-I, bd-II and bo3 were isolated from the E. coli strains GO105/pTK1, MB37 and GO105/pJRhisA, respectively, as previously described39 (link)40 (link)41 (link). The concentration of the cytochromes bd-I and bd-II was determined from the difference absorption spectrum using Δε628-607 = 10.8 mM−1 cm−1 for the dithionite-reduced minus ‘as prepared’ proteins. Cytochrome bo3 concentration was estimated from the Soret absorption band of the oxidized enzyme using ε407 = 183 mM−1 cm−1. UV-visible absorption spectra were acquired in an Agilent Cary 60 spectrophotometer.
5
Spectroscopic Characterization of Samples
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UV-Vis absorption spectra were registered at 20 °C using an Agilent Cary 60 spectrophotometer (Agilent Technologies, Waldbronn, Germany).
FT-IR spectra were collected on a Bruker Vertex 70 (Bruker Daltonik GmbH, Bremen, Germany) spectrometer connected with a Platinium ATR, Bruker Diamond Type A225/Q. The samples spectra were collected after 64 co-added scans, on a spectral domain of 4000–400 cm−1, with a resolution of 4 cm−1.
FT-IR spectra were collected on a Bruker Vertex 70 (Bruker Daltonik GmbH, Bremen, Germany) spectrometer connected with a Platinium ATR, Bruker Diamond Type A225/Q. The samples spectra were collected after 64 co-added scans, on a spectral domain of 4000–400 cm−1, with a resolution of 4 cm−1.
6
Quantifying Polymer Concentration via UV-Vis
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The UV-Vis method has proven to be trusted and convenient for measuring the concentration of polymers [30 (link),31 (link)]. Agilent Cary 60 Spectrophotometer with a wavelength between 1100–190 nm (Agilent Technologies, Santa Clara, CA, USA) was used to determine the polymer concentration in both dynamic and static tests. Cary 60 (Agilent Technologies) was utilized to measure the absorbance of the polymer as a function of the wavelength (peaking at 330–300 nm) of the electromagnetic spectrum.
7
Cuticle Analysis of Plant Seeds
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Toluidine blue (TBO) staining is known to examine the cuticular properties of plant85 (link). Experiments were performed as described earlier86 ,87 (link) using seeds88 (link). TBO solution was prepared with 10 mM sodium citrate, pH 4.4; 0.05w/v toluidine blue and 0.4 v/v tween 20. Ten seeds each of wild type and rga1 were immersed in the solution in microcentrifuge tubes and incubated at room temperature for not more than 3 min. They were gently rinsed with water with a pipette for 3 min to remove unbound stain. They were treated with 80% ethanol and incubated at 37 ºC for 2 h and absorbance readings were taken at 626 and 430 nm using Agilent Cary 60 spectrophotometer.
8
Purification and Characterization of Organic Solvents
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Acetone, dichloromethane (DCM), hexane, 2-propanol (iPrOH), toluene, ethyl-acetate (EtOAc) (reagent grade, Molar Chemicals, Hungary) were purified by distillation. Acetonitrile (MeCN), tetrahydrofuran (THF), methanol (MeOH), dimethyl formamide (DMF), dimethyl sulfoxide (DMSO), pyridine (HPLC grade, VWR, Germany), cyclohexane, 1,4-dioxane (reagent grade, Reanal, Hungary), chloroform, 1,5-diaminoanthraquinone (Sigma-Aldrich, Germany) were used without further purification.
NMR: 1H and 13C-NMR spectra were recorded in CDCl3 and DMSO-d6 at 25 °C on a Bruker Avance DRX-400 and a Bruker AM 360 spectrometer at 400 and 360 MHz, respectively, with tetramethylsilane as the internal standard.
UV–vis: The UV–vis spectra were recorded on an Agilent Cary 60 spectrophotometer (Agilent, Santa Clara, CA, USA) in a quartz cuvette of 1.00 cm optical length. A 3.00 cm3 solution was prepared from the sample.
NMR: 1H and 13C-NMR spectra were recorded in CDCl3 and DMSO-d6 at 25 °C on a Bruker Avance DRX-400 and a Bruker AM 360 spectrometer at 400 and 360 MHz, respectively, with tetramethylsilane as the internal standard.
UV–vis: The UV–vis spectra were recorded on an Agilent Cary 60 spectrophotometer (Agilent, Santa Clara, CA, USA) in a quartz cuvette of 1.00 cm optical length. A 3.00 cm3 solution was prepared from the sample.
9
Extraction and Quantification of Total Phenolics in EVOO
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Total phenolic compounds of the EVOO samples were extracted and determined according to Caponio et al. (Caponio et al., 2015) (link) Briefly, extraction was carried out on 1 g of oil by adding 1 mL of hexane and 5 mL of methanol/water (70:30 v/v). After vortexing for 10 min and centrifuging at 6,000 rpm for 10 min at 4 °C (Beckman Coulter, Fullerton, California, USA), the hydroalcoholic phase was recovered, centrifuged again at 9,000 rpm for 5 min at 4 °C and filtered through nylon filters (pore size 0.45 μm, Sigma-Aldrich, Milan, Italy). Then, 100 µL of extract were mixed with 100 µL of Folin-Ciocalteu reagent and, after 4 min, with 800 µL of a 5% (w/v) solution of sodium carbonate. The mixture was then heated in a water bath at 40 °C for 20 min and the total phenol content was determined at 750 nm by an Agilent Cary 60 spectrophotometer (Agilent Technologies, Santa Clara, USA). The total phenolic content was expressed as gallic acid equivalents (mg/kg).
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