Ppar r

Dendrobium officinale was purchased from Zhejiang Shouxiangu Pharmaceutical Co., Ltd. (Jinhua, China), and PMA and LPS were purchased from Sigma (Saint Louis, MI, USA). Recombinant human interleukin-4 (IL-4) was purchased from PeproTech, Inc. (Rocky Hill, NJ, USA). TNF-α and IL-6 ELISA kits were purchased from Lianke Biological Technology (Hangzhou, China). Cell Counting Kit-8 (CCK-8) Assay Kit was received from MCE (Romulus, NJ, USA). Total Protein Extraction kit was purchased from Beyotime (Shanghai, China). The MiNiBEST Universal RNA Extraction Kit, PrimeScript RT Reagent kit, and SYBR Premix Ex Taq II Kit were purchased from TaKaRa (Dalian, China). Transwell polycarbonate membrane had an 8 μm pore size (Corning City, NY, USA). The primary antibodies E-cadherin, N-cadherin, Vimentin, Caspase-3, Bax, Bcl-2, Ki67, and β-actin were purchased from Proteintech (Wuhan, China). The primary antibodies ARG1, TGM2, and Cleaved-NOTCH1 were purchased from Cell Signaling Technology (Danvers, MA, USA). The primary antibodies STAT6, p-STAT6, PPAR-r, JAGGED1, Cleaved-NOTCH1, and NOTCH1 were purchased from Abcam (Cambridge, UK). β-actin was purchased from Proteintech (Wuhan, China). The ECL Plus Western Blotting Detection Kit was purchased from Technology Co., Ltd. (Beijing, China). Anti-CD80-FITC and Anti-CD206-PE were obtained from Thermo Fisher (Waltham, MA, USA).

Total protein lysates were obtained from harvested cells in protein RIPA lysis buffer (Beyotime, Shanghai, China) containing 10 mM phenylmethylsulphonyl fluoride as a protease inhibitor (PMSF; Beyotime) on ice for 30 min. The concentrations of total proteins were measured via Coomassie blue staining. The total proteins were separated on 10% SDS-PAGE gels and transferred to 0.45 m Immobilon-P Transfer Membranes (Millipore Corporation, Billerica, MA, USA). The membranes were blocked in 5% skim milk dissolved in Tris-buffered saline containing Tween and then immunoblotted with primary antibodies. Antibodies used in this experiment include: β-actin, GAPDH, RUNX2, ALP, OCN, SP7, PPAR-r (Abcam plc, Cambridge, UK), HDAC9, H3K9, H3K14, H3K18, H4K16 (R&D Systems, Minneapolis, MN, USA), and HRP-conjugated secondary antibodies to rabbit and mouse (Santa Cruz Inc., CA, USA).