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Nad nadh kit

Manufactured by Abcam
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The NAD/NADH kit is a biochemical assay designed to quantify the levels of NAD (nicotinamide adenine dinucleotide) and NADH (the reduced form of NAD) in biological samples. The kit provides a simple and sensitive method for measuring the concentrations of these important coenzymes, which play crucial roles in cellular metabolism and energy production.

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Lab products found in correlation

Spectramax m2, by Molecular Devices (3 mentions) Elx800, by Agilent Technologies (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Nadp nadph kit, by Abcam (1 mentions) Cobas 8000, by Roche (1 mentions)

Close spelling variants of this product

NAD+/NADH colorimetric kit NAD/NADH assay kit NAD+/NADH Quantification assay Kit NADH/NAD determination kit NAD+/NADH Quantitation Kit NAD+/NADH Ratio Assay kit NAD/NADH Quanti cation Colorimeteric Kit NAD+/NADH quantification kit Biovision NAD/NADH quantification colorimetric assay kit NAD+/NADH Colorimetric Assay Kit

8 protocols using nad nadh kit

1

NAD+ Measurement Protocol

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Cells of 2 × 106 for each sample were harvested through scraping, washed in PBS (Gibco), and suspended in a tube by spinning at 2,000 rpm for 5 min. NAD+ was measured by the NAD+/NADH kit (ab65348, Abcam). Colorimetric measurements were completed at 450 nm absorbance through a microplate reader (ELX800, BioTek).
Gu X., Hua Y., Yu J., Yang L., Ge S., Jia R., Chai P., Zhuang A, & Fan X. (2022). Epigenetic drug library screening reveals targeting DOT1L abrogates NAD+ synthesis by reprogramming H3K79 methylation in uveal melanoma. Journal of Pharmaceutical Analysis, 13(1), 24-38.
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2

Intracellular NAD Quantification

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Intracellular NAD pools were measured using NAD/NADH kit from Abcam (ab65348) according to the manufacturer’s instructions.
Chowdhry S., Zanca C., Rajkumar U., Koga T., Diao Y., Raviram R., Liu F., Turner K., Yang H., Brunk E., Bi J., Furnari F., Bafna V., Ren B, & Mischel P.S. (2019). NAD metabolic dependency in cancer is shaped by gene amplification and enhancer remodeling. Nature, 569(7757), 570-575.
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3

Measurement of Cellular Redox Cofactors

Cited 1 time
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Production of NADH and NADPH was measured using, respectively, the NAD/NADH kit (Abcam, Cambridge, UK) and the NADP/NADPH kit (Abcam) according to the manufacturer's instruction. Lactate and LDH activity were measured using the Roche diagnostic kit on a Cobas 8000 (Roche Diagnostics, Mannheim, DE, USA) as described previously.39 (link)
Oizel K., Gratas C., Nadaradjane A., Oliver L., Vallette F.M, & Pecqueur C. (2015). D-2-Hydroxyglutarate does not mimic all the IDH mutation effects, in particular the reduced etoposide-triggered apoptosis mediated by an alteration in mitochondrial NADH. Cell Death & Disease, 6(3), e1704-.
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4

Cellular NAD+ Quantification Protocol

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For measurement of cellular NAD+ levels, 250,000 cells were plated into six‐well plates and 24 h later drug treatment was initiated. 48 h later cells were processed using the NAD/NADH kit (Abcam) according to the manufacturer’s instructions.
Bajrami I., Walker C., Krastev D.B., Weekes D., Song F., Wicks A.J., Alexander J., Haider S., Brough R., Pettitt S.J., Tutt A.N, & Lord C.J. (2021). Sirtuin inhibition is synthetic lethal with BRCA1 or BRCA2 deficiency. Communications Biology, 4, 1270.
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5

Quantification of NAD and NADH

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NAD/NADH was measured in heart tissue using NAD/NADH kit (Abcam, Cambridge, UK) according to the manufacturer's instruction. Absorbance was measured at 450 nm using microplate reader/multidetection reader (SPECTRAMAX M2, Molecular Devices, Wokingham, UK). Total NAD (NADt) and NADH were estimated directly while the value of NAD+ was estimated by subtracting NADH from NADt.
Mohammed Abdul K.S., Jovanović S., Du Q., Sukhodub A, & Jovanović A. (2015). Mild hypoxia in vivo regulates cardioprotective SUR2A: A role for Akt and LDH. Biochimica et Biophysica Acta, 1852(5), 709-719.
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6

Quantifying Colonic NAD+/NADH Levels

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NAD+/NADH levels in the colonic epithelium were assessed by using a NAD+/NADH Kit (Abcam) following the manufacturer’s instructions. Intestinal epithelial scrapings were homogenized in NAD+ Extraction Buffer (provided in the kit) supplemented with protease and phosphatase inhibitors. NAD+ levels were normalized to the protein concentration of the protein lysates prior to NAD+/NADH extraction.
Novak E.A., Crawford E.C., Mentrup H.L., Griffith B.D., Fletcher D.M., Flanagan M.R., Schneider C., Firek B., Rogers M.B., Morowitz M.J., Piganelli J.D., Wang Q, & Mollen K.P. (2023). Epithelial NAD+ depletion drives mitochondrial dysfunction and contributes to intestinal inflammation. Frontiers in Immunology, 14, 1231700.
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7

Quantifying NAD and NADH in Heart

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NAD/NADH was measured in heart tissue using NAD/NADH kit (Abcam, Cambridge, UK) according to the manufacturer's instruction. Absorbance was measured at 450 nm using microplate reader/multidetection reader (SpectraMax M2; Molecular Devices). Total NAD (NADt) and NADH were estimated directly while the value of NAD+ was estimated by subtracting NADH from NADt.
Mohammed Abdul K.S., Jovanović S, & Jovanović A. (2017). Exposure to 15% oxygen in vivo up‐regulates cardioprotective SUR2A without affecting ERK1/2 and AKT: a crucial role for AMPK. Journal of Cellular and Molecular Medicine, 21(7), 1342-1350.
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8

Quantification of NAD and NADH

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NAD/NADH was measured in heart tissue using NAD/NADH kit (Abcam, Cambridge, UK) according to the manufacturer's instruction. Absorbance was measured at 450 nm using microplate reader/multidetection reader (SPECTRAMAX M2, Molecular Devices, Wokingham, UK). Total NAD (NADt) and NADH were estimated directly while the value of NAD+ was estimated by subtracting NADH from NADt.
Mohammed Abdul K.S., Jovanović S., Sukhodub A., Du Q, & Jovanović A. (2014). Upregulation of cardioprotective SUR2A by sub-hypoxic drop in oxygen. Biochimica et Biophysica Acta. Molecular Cell Research, 1843(11), 2424-2431.
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