Sample purific cv hiv 1 extraction kit

The CD4⁺ and CD8⁺ T lymphocyte counts were performed at the Virology Laboratory, as established by the National Network of CD4⁺/CD8⁺ T lymphocytes of the Ministry of Health. The flow cytometry methodology was used, which evaluated a whole blood sample with EDTA, using BD FACScount equipment and BD multi-test reagents (CD45, CD3, CD4, and CD8) (BD, Franklin Lakes, NJ, USA), following the protocol recommended by the manufacturer.
The plasma viral load was determined at the Virology Laboratory, following the standard methodology of the National Viral Load Network of the Ministry of Health, based on qPCR technology, using the Sample Purific CV HIV-1 Extraction Kit, the HIV-1 Viral Load Amplification, and the Abbott 2000mrt thermal cycler (ABBOTT, Chicago, IL, USA) and all steps were followed according to the manufacturer’s recommendations. Viral load measurements were used in log10.

A viral load quantification was performed using real-time polymerase chain reaction (PCR) amplification technology, the Sample Purific CV HIV-1 extraction kit (Abbott) and the HIV-1 viral load amplification kit (Abbott, Chicago, IL, USA). The viral load results were expressed as copies/mL and log10.

The viral load was quantified by real-time PCR using the Sample Purific CV HIV-1 extraction kit (Abbott) and the HIV-1 viral load amplification kit (Abbott, Chicago, Illinois, USA). The units used were copies/mL converted by log₁₀. Both the CD4⁺ and CD8⁺ T cells and the HIV-1 viral load were quantified according to the standard set by the National Network for the Determination of CD4⁺ and CD8⁺ T cells and Viral Load of the Department of HIV/AIDS and Viral Hepatitis of the Ministry of Health.