Primary antibody for collagen 1

Manufactured by Abcam

Primary antibody for collagen I. Binds to collagen I protein.

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Lab products found in correlation

Polymyxin b agarose beads, by Merck Group (1 mentions) Limulus amebocyte lysate assay kit, by Lonza (1 mentions) Tak 242, by MedChemExpress (1 mentions)

Spelling variants of this product

Collagen I (278 citations) Collagen I antibody (6 citations)

2 protocols using primary antibody for collagen 1

Schistosoma japonicum Egg Antigen Protocols

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SEA of S. japonicum and S. japonicum cercariae were obtained from Jiangsu Institute of Parasitic Diseases (China). SEA was sterile-filtered and endotoxin was removed with Polymyxin B agarose beads (Sigma, USA). Limulus amebocyte lysate assay kit (Lonza, Switzerland) was used to confirm the removal of endotoxins from the SEA as previously described29 (link). Primary antibodies for α-SMA, p53, p21, p16, Toll-like receptor (TLR) 4, SOCS3 and STAT3 were purchased from Santa Cruz Biotechnology (USA). Primary antibody for collagen I was purchased from Abcam. Primary antibodies for phospho-p53 (Ser15) and phospho-STAT3 (Tyr-705) were purchased from Cell Signaling Technology (USA). All of the secondary antibodies were obtained from Santa Cruz Biotechnology (USA). TAK-242, a small-molecule-specific inhibitor of TLR4 signaling, was purchased from Medchemexpress.

Chen J., Pan J., Wang J., Song K., Zhu D., Huang C, & Duan Y. (2016). Soluble egg antigens of Schistosoma japonicum induce senescence in activated hepatic stellate cells by activation of the STAT3/p53/p21 pathway. Scientific Reports, 6, 30957.

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Immunofluorescence Staining of Scaffolds

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For immunofluorescence, scaffolds were washed with PBS and fixed with formalin for 15 minutes at room temperature. Scaffolds were washed with cold PBS for 5 minutes, twice. Scaffolds were permeabilized with 0.25% TritonX100 solution and incubated for 10 minutes. Triton was removed, and scaffolds were again washed with cold PBS for 5 minutes. The scaffolds were blocked with 3% BSA for 30 minutes at room temperature. The BSA solution was then removed, and primary antibody for collagen I (rabbit polyclonal; Abcam) was added in 1%BSA solution for 1 hour. The primary antibody was removed, and the scaffold was washed thrice in PBS for 5 minutes. Secondary antibody was then added for 40 minutes, followed by subsequent PBS washing step, for 5 minutes, thrice. Dapi staining was added prior to viewing.

Ramos D.M., Abdulmalik S., Arul M.R., Rudraiah S., Laurencin C.T., Mazzocca A.D, & Kumbar S.G. (2019). Insulin immobilized PCL-cellulose acetate micro-nanostructured fibrous scaffolds for tendon tissue engineering. Polymers for advanced technologies, 30(5), 1205-1215.

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