Anti myc antibody m4439

Manufactured by Merck Group

Sourced in United States

The Anti-Myc antibody (M4439) is a laboratory reagent used to detect the presence of the Myc protein in various biological samples. It is a monoclonal antibody that specifically recognizes the Myc epitope, which is a short protein sequence commonly used as a tag in recombinant proteins. The antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunocytochemistry to identify and study Myc-tagged proteins.

Automatically generated - may contain errors

Lab products found in correlation

Anti β actin antibody a2228, by Merck Group (1 mentions) Dapi d1306, by Thermo Fisher Scientific (1 mentions) Lps e coli serotype 055 b5, by Merck Group (1 mentions) Goat anti mouse igg 31430, by Thermo Fisher Scientific (1 mentions) Anti irak1 sc 5288, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg 31460, by Thermo Fisher Scientific (1 mentions) C25 140 hy 120934, by MedChemExpress (1 mentions) Anti flag antibody f3165, by Merck Group (1 mentions) Protein a mag sepharose bead suspension, by GE Healthcare (1 mentions)

Spelling variants of this product

Anti-Myc (274 citations) Anti-Myc antibody (109 citations) M4439 (48 citations) Anti-Myc (M4439, (7 citations)

3 protocols using anti myc antibody m4439

Immunostaining of Tailor and dmDis3l2 in S2 cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunostaining of S2 cells was performed as previously described (Reimão-Pinto et al, 2015 (link)). For co-imaging of Tailor and dmDis3l2, S2 cells were transiently transfected with pAFMW-Tailor and pAGW-dmDis3l2. Anti-Myc antibody (M4439, Sigma) was used at 1:2000 dilution. Anti-dmDis3l2 was used at 1:150 dilution.

Reimão‐Pinto M.M., Manzenreither R.A., Burkard T.R., Sledz P., Jinek M., Mechtler K, & Ameres S.L. (2016). Molecular basis for cytoplasmic RNA surveillance by uridylation‐triggered decay in Drosophila. The EMBO Journal, 35(22), 2417-2434.

+ Open protocol

+ Expand

Signaling Pathway Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

LPS (E. coli, serotype 055:B5), anti-Flag antibody (F3165), anti-Myc antibody (M4439) and anti-β-actin antibody (A2228) were purchased from Sigma. Poly(I:C), R-848 and ODN 1585 were from Enzo Life Sciences. DAPI (D1306) were purchased from Invitrogen. Antibody to p-IKKβ (2694), IKKβ (2678), p-JNK (4668), JNK (9252), p-ERK (4376), ERK (4695), p-IκBα (9246), IκBα (4812), MyD88 (4283), HA (3724), TRAF6 (4743; immunofluorescence analysis), and Sufu (2522) were purchased from Cell Signaling. Anti-His (sc-8036), anti-TRAF6 (sc-8409) (immunoprecipitation and immunoblot analysis), anti-IRAK1 (sc-5288) and anti-IRAK4 (sc-374349) were purchased from Santa Cruz. Goat anti-mouse IgG (31430) and goat anti-rabbit IgG (31460) were purchased from Thermo Fisher Scientific. C25-140 (HY-120934) was purchased from MedChemExpress.

Li Y., Peng J., Xia Y., Pan C., Li Y., Gu W., Wang J., Wang C., Wang Y., Song J., Zhou X., Ma L., Jiang Y., Liu B., Feng Q., Wang W., Jiao S., An L., Li D., Zhou Z, & Zhao Y. (2023). Sufu limits sepsis-induced lung inflammation via regulating phase separation of TRAF6. Theranostics, 13(11), 3761-3780.

+ Open protocol

+ Expand

FBXO11 Protein Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells were transiently transfected with wildtype or mutant FBXO11 plasmids (1.5 μg per 6 well). Cells were scraped from the culture dish in lysis buffer (100 mm TRIS–HCl pH 8, 150 mm NaCl, 1 mm EDTA, 1% Triton X-100). Immunoprecipitation was performed with Protein A Mag Sepharose bead suspension (GE Healthcare, Boston, MA, USA), incubated with the sample and anti-Myc antibody (M4439, Sigma-Aldrich, St. Louis, MO, USA) at 4°C overnight. Subsequently, beads were washed in lysis buffer, and samples were eluted with 1x Lämmli buffer. SDS-page and western blotting was performed as described previously.

Gregor A., Meerbrei T., Gerstner T., Toutain A., Lynch S.A., Stals K., Maxton C., Lemke J.R., Bernat J.A., Bombei H.M., Foulds N., Hunt D., Kuechler A., Beygo J., Stöbe P., Bouman A., Palomares-Bralo M., Santos-Simarro F., Garcia-Minaur S., Pacio-Miguez M., Popp B., Vasileiou G., Hebebrand M., Reis A., Schuhmann S., Krumbiegel M., Brown N.J., Sparber P., Melikyan L., Bessonova L., Cherevatova T., Sharkov A., Shcherbakova N., Dabir T., Kini U., Schwaibold E.M., Haack T.B., Bertoli M., Hoffjan S., Falb R., Shinawi M., Sticht H, & Zweier C. (2021). De novo missense variants in FBXO11 alter its protein expression and subcellular localization. Human Molecular Genetics, 31(3), 440-454.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!