The efficacy of HOCl against MS2 infectivity was tested using dried MS2 on stainless steel coupons submerged in HOCl and by spray delivery of HOCl. MS2 bacteriophage (ATCC 15597-B1) was prepared by confluent lysis on E. coli F-amp (ATCC 70081) in 15 mL soft agar (0.5% Bacto agar and 0.7% NaCl) poured onto a nutrient agar petri dish and incubated at 37°C overnight. Propagated MS2 was further extracted via organic solvent extraction using Vertrel XF (Dupont, Wilmington, DE) by vigorously mixing scraped top layer of soft agar and equal volumes of Vertrel XF followed by centrifugation at 3,500 × g at 4°C for 15 minutes. Centrifuged supernatant was collected and stored in 1 mL aliquots at −80°C. All MS2 stocks and samples were quantified by 10-fold serial dilutions and plating using the double agar layer (DAL) method as previously described.26
Vertrel xf
Manufactured by DuPont
Vertrel® XF is a specialty solvent developed by DuPont. It is a non-flammable, low-toxicity fluorinated compound designed for use in various industrial applications. The product's core function is to provide a cleaning and degreasing solution for precision cleaning and other industrial processes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Amphotericin b, by Merck Group (2 mentions)
Qiaamp viral rna mini kit, by Qiagen (1 mentions)
14 protocols using vertrel xf
1
Evaluating HOCl Efficacy Against MS2 Virus
2
Lubricant Coating Optimization for Magnetic Disks
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Example 3
Compound 4 and a mixture of Compound 1 and Compound 4 (15 wt %: 85 wt %) were each dissolved in Vertrel-XF, produced by DuPont. The concentration of the lubricant in each solution was 0.05 wt %. Magnetic disks having a diameter of 2.5 inches were individually dipped in each solution for 1 minute, and lifted out at 2 mm/s. Subsequently, a magnetic head was mounted, and the prepared magnetic disks were each rotated at a high speed of 5400 rpm. Thereafter, the amount of the lubricant adhering to the magnetic head was measured.
These results confirmed that the lubricant comprising a fluoropolyether-based lubricant and the compound of the present invention having an alkoxy group at one terminal and a hydroxyl group at the other terminal could reduce the pickup.
3
Bacteriophage Propagation and Purification
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Three bacteriophages were used as model viruses: fr, P22, and ΦX174. The properties of these bacteriophages are summarized in Table 1 . Bacteriophages were propagated using the double-agar layer (DAL) method [32 ], followed by two cycles of polyethylene glycol (PEG) precipitation, followed by a Vertrel XF (DuPont, Wilmington, DE) purification, as previously described by Mayer et al. [33 (link)].
4
Formulation of Hydrophobic Insulating Liquids
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Example 2
The following example provides a method for preparing a mixture of hydrophobic liquids that have electrical insulation capabilities by mixing two or more hydrophobic liquids in a solvent using one of many known agitation techniques.
A mixture containing 10% by weight phenylated siloxane, 20% by weight fluoroalkane, and 70% by weight of a hydro-fluorocarbon (sold under the name DuPont™ Vertrel® XF, which is HFC 43-10mee or 2,3-dihydrodecafluoropentane; empirical formula C5H2F10) were mixed together at 25° C. using an impeller mixer at 2000 rpm for 30 minutes, or until all three components formed a homogenous mixture.
5
Murine L929 Cell Culture and Reovirus Propagation
6
Preparation of Hydrophobic Insulation Liquid
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Example 2
The following example provides a method for preparing a mixture of hydrophobic liquids that have electrical insulation capabilities by mixing two or more hydrophobic liquids in a solvent using one of many known agitation techniques.
A mixture containing 10% by weight phenylated siloxane, 20% by weight fluoroalkane, and 70% by weight of a hydro-fluorocarbon (sold under the name DuPont™ Vertrel® XF, which is HFC 43-10 mee or 2,3-dihydrodecafluoropentane; empirical formula C5H2F10) were mixed together at 25° C. using an impeller mixer at 2000 rpm for 30 minutes, or until all three components formed a homogenous mixture.
7
Hydrophobic Liquid-Solid Particle Mixture
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Example 3
The following example provides a method for preparing a mixture of hydrophobic liquids with solid particles that both have electrical insulation capabilities by mixing a two or more hydrophobic liquids with two or more hydrophobic particles using one of many known agitation techniques.
A mixture containing 10% by weight phenylated siloxane, 20% by weight fluoroalkane, 5% by weight precipitated hydrophobically treated silica, and 65% by weight of a hydro-fluorocarbon (sold under the name DuPont™ Vertrel® XF) were mixed together using a rotor-stator homogenizer.
The phenylated siloxane, the fluoroalkane, and the hydro-fluorocarbon solvent were first mixed together at 25° C. using a roto-stator mixer at 10,000 rpm for 5 minutes until a homogenous solution was obtained.
The solid hydrophobically treated precipitated silica was then introduced in increment. The rotor-stator was run at 10,000 rpm every time the silica was introduced. The process was repeated until a homogenous dispersion was obtained.
Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with the true scope of the invention being indicated by the following claims.
8
Preparation of Hydrophobic Insulating Mixture
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Example 2
The following example provides a method for preparing a mixture of hydrophobic liquids that have electrical insulation capabilities by mixing two or more hydrophobic liquids in a solvent using one of many known agitation techniques.
A mixture containing 10% by weight phenylated siloxane, 20% by weight fluoroalkane, and 70% by weight of a hydro-fluorocarbon (sold under the name DuPont™ Vertrel® XF, which is HFC 43-10mee or 2,3-dihydrodecafluoropentane; empirical formula C5H2F10) were mixed together at 25° C. using an impeller mixer at 2000 rpm for 30 minutes, or until all three components formed a homogenous mixture.
9
Reovirus Strain Purification and Quantification
10
Preparation of Hydrophobic Liquid-Solid Insulator Mixture
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Example 3
The following example provides a method for preparing a mixture of hydrophobic liquids with solid particles that both have electrical insulation capabilities by mixing a two or more hydrophobic liquids with two or more hydrophobic particles using one of many known agitation techniques.
A mixture containing 10% by weight phenylated siloxane, 20% by weight fluoroalkane, 5% by weight precipitated hydrophobically treated silica, and 65% by weight of a hydro-fluorocarbon (sold under the name DuPont™ Vertrel® XF) were mixed together using a rotor-stator homogenizer.
The phenylated siloxane, the fluoroalkane, and the hydro-fluorocarbon solvent were first mixed together at 25° C. using a roto-stator mixer at 10,000 rpm for 5 minutes until a homogenous solution was obtained.
The solid hydrophobically treated precipitated silica was then introduced in increment. The rotor-stator was run at 10,000 rpm every time the silica was introduced. The process was repeated until a homogenous dispersion was obtained.
Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with the true scope of the invention being indicated by the following claims.
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