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Tridecafluoro 1 1 2 2 tetrahydrooctyl trichlorosilane

Manufactured by Merck Group

Tridecafluoro-1,1,2,2-tetrahydrooctyl trichlorosilane is a specialty chemical compound used in the production of various laboratory equipment and tools. It serves as a key component in the manufacturing process, providing specific functional properties required for the intended applications. The detailed description and intended use of this product will be provided upon request.

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3 protocols using tridecafluoro 1 1 2 2 tetrahydrooctyl trichlorosilane

1

Surface Silanization of 3D Printed Parts

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Where noted, some printed parts were plasma treated using a BD-20AC laboratory corona treater (Electro-Technic Products, Chicago IL, USA). Printed parts were placed 3 mm below the plasma source and treated for 5 – 60 s immediately prior to surface silanization. For gas-phase deposition, 200 μL of neat tridecafluoro-1,1,2,2-tetrahydrooctyl trichlorosilane (Gelest Inc., Morrisville PA, USA) was placed in a vacuum desiccator in a small polypropylene dish, followed immediately by the printed parts, and a vacuum was applied for 2 hours at room temperature. For solvent deposition, the surface of the printed part was submerged in a 10% v/v solution of tridecafluoro-1,1,2,2-tetrahydrooctyl trichlorosilane in solvent (Fluorinert FC-40 (Sigma Aldrich) or 200 proof ethanol (Koptec) for 30 min at room temperature, unless otherwise specified. After silanization, surfaces were rinsed with 95% ethanol and DI water and dried with a nitrogen gun.
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2

Fabrication and Characterization of Microfluidic Devices

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Certus Prestige OPTIMA Investment Powder was purchased from American Jewelry Supply (East Brunswick, NJ). More details can be found from: https://pepetools.com/products/certus-investment-powder-prestige-optima-49lb-bag. Sylgard 184 poly(dimethylsiloxane) kits were purchased from Dow Corning (Midland, MI). ZEONEX 1420R Cyclo Olefin Polymer (Tg=135 °C) was obtained from ZEONEX (San Jose, CA). WarmStart LAMP Kits and bovine serum albumin (BSA) were purchased from New England BioLabs (Ipswich, MA). High-purity calcein was obtained from Thermo Fisher (Waltham, MA). Betaine, Tween-20, light mineral oil, (tridecafluoro-1,1,2,2-tetrahydrooctyl)trichlorosilane, and MnCl2 were obtained from Sigma-Aldrich (St. Louis, MO). Abil WE 09 and Tegosoft DEC were purchased from UPI Chem (Somerset, NJ). ClearWeld 5 Minute Epoxy (AB glue) was purchased from J-B Weld.
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3

Biofunctionalized Surfaces for Biosensing

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Poly-l-lysine hydrobromide (MW =
15–30 kDa by viscosity), EDC, NHS, tablets for 10 mM PBS solution
(pH 7.4), 3-trichlorosilylpropyl methacrylate, 2-hydroxyethyl methacrylate
(HEMA), ethylene glycoldimethacrylate (EGDMA), tridecafluoro-(1,1,2,2)-tetrahydrooctyl-trichlorosilane,
bovine serum albumin, ammonium persulfate (APS), and NaCl salt were
purchased from Sigma-Aldrich. Methyl-OEG4-NHS ester, biotin-OEG4-NHS ester, Mal-OEG4-NHS ester, the Zeba spin desalting
columns (7 kDa MWCO, 5 mL), the immobilized TCEP disulfide reducing
gel (tris[2-carboxyethyl] phospine hydrochloride immobilized onto
4%cross-linked beaded agarose), and the streptavidin-Fluorescein conjugate
were obtained from ThermoFisher Scientific. Oligonucleotides were
purchased from Eurofins Genomics and used as received. SiO2 QCM chips (with fundamental frequency of 5 MHz) were purchased from
Biolin Scientific. Poly(dimethylsiloxane) Sylgard 184 and curing agent
were used as received from Dow Corning. Ormostamp material and MA-T1050
were purchased from Microresist. The PNA probes were synthesized using
the materials and the procedure previously described for PNA2,52 (link) as briefly reported in the Supporting Information together with the characterization
of the newly synthesized PNA3 and PNA4.
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