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Pcmv6 entry fads2 myc ddk plasmid

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The PCMV6-Entry-FADS2-Myc-DDK plasmid is a recombinant DNA construct that contains the coding sequence for the FADS2 gene, which encodes the fatty acid desaturase 2 enzyme. The plasmid features a CMV promoter, an entry vector backbone, and Myc-DDK tags for protein detection and purification purposes. This plasmid can be used for various molecular biology applications, such as gene expression studies, protein production, and functional analysis of the FADS2 gene.

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Lab products found in correlation

Rneasy micro kit, by Qiagen (2 mentions) Sybr green, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

PCMV6-Entry (97 citations) PCMV6-ddk-myc (21 citations) PCMV6-Entry plasmid (12 citations) PCMV6 plasmids (2 citations)

2 protocols using pcmv6 entry fads2 myc ddk plasmid

1

Quantitative PCR and Immunoblot Analyses of FADS2

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For quantitative PCR, RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer’s protocols. Transcripts were reverse transcribed according to manufacturer protocols (Invitrogen), and qPCR was performed using SYBR Green (Applied Biosystems). The following primers were used: mouse FADS2, F-5’-gctctcagatcaccgaggac-3’, R-5’-agtgccgaagtacgagagga-3’. Immunoblot for different tissues (100μg total protein) was performed in nitrocellulose membranes after tissue sonication in RIPA buffer. The pCMV6-Entry-FADS2-Myc-DDK plasmid was purchased from Origene (MR207091), single point mutation of FADS2 42K to Q and A was performed at GenScript.
Dong S., Wang Q., Kao Y., Diaz A., Tasset I., Kaushik S., Thiruthuvanathan V., Zintiridou A., Nieves E., Dzieciatkowska M., Reisz J., Gavathiotis E., D’Alessandro A., Will B, & Cuervo A. (2021). Chaperone-mediated autophagy sustains hematopoietic stem cell function. Nature, 591(7848), 117-123.
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2

Quantitative PCR and Immunoblot Analyses of FADS2

Check if the same lab product or an alternative is used in the 5 most similar protocols
For quantitative PCR, RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer’s protocols. Transcripts were reverse transcribed according to manufacturer protocols (Invitrogen), and qPCR was performed using SYBR Green (Applied Biosystems). The following primers were used: mouse FADS2, F-5’-gctctcagatcaccgaggac-3’, R-5’-agtgccgaagtacgagagga-3’. Immunoblot for different tissues (100μg total protein) was performed in nitrocellulose membranes after tissue sonication in RIPA buffer. The pCMV6-Entry-FADS2-Myc-DDK plasmid was purchased from Origene (MR207091), single point mutation of FADS2 42K to Q and A was performed at GenScript.
Dong S., Wang Q., Kao Y., Diaz A., Tasset I., Kaushik S., Thiruthuvanathan V., Zintiridou A., Nieves E., Dzieciatkowska M., Reisz J., Gavathiotis E., D’Alessandro A., Will B, & Cuervo A. (2021). Chaperone-mediated autophagy sustains hematopoietic stem cell function. Nature, 591(7848), 117-123.
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