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Anti active β catenin d13a1

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Anti-active β-catenin (D13A1) is a rabbit monoclonal antibody that specifically recognizes the active, dephosphorylated form of β-catenin. β-catenin is a key mediator of the Wnt signaling pathway and plays a critical role in cell-cell adhesion and gene transcription.

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Lab products found in correlation

Background punisher, by Biocare Medical (2 mentions) High ph 9, by Vector Laboratories (2 mentions) Anti ki67 antibody d2h10, by Cell Signaling Technology (2 mentions) Anti krt5 antibody poly 19055, by BioLegend (2 mentions) Anti erα c 311, by Santa Cruz Biotechnology (2 mentions) Rabbit or mouse on rodent polymers, by Biocare Medical (2 mentions) Anti pr d8q2j, by Cell Signaling Technology (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions)

Spelling variants of this product

β-catenin (1138 citations) Anti-β-catenin (433 citations) Active β-catenin (74 citations) Anti-active β-catenin (34 citations)

3 protocols using anti active β catenin d13a1

1

Immunohistochemical Analysis of Mammary Ducts

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Tissues were processed with standard IHC protocols. High pH 9 (Vector Labs) was used for antigen retrieval and blocked with background punisher (BioCare Medical, Concord CA). Slides were incubated with primary antibody, anti Ki67 antibody (D2H10; Cell signaling), anti-KRT5 antibody (Poly 19055; Biolegend, San Diego, CA), anti-active β-catenin (D13A1; Cell Signaling), anti-ERα (C-311; Santa Cruz Biotechnology, Dallas, Texas), or anti-PR (D8Q2J; Cell Signaling) for 2 h. Next, rabbit or mouse-on-rodent polymers (BioCare Medical) for 30 min, developed with 3,3’-diaminobenzadine (DAB and 0.3% H2O2 in PBS) developing buffer, and counterstained. Positive cells and total cells per mammary duct were counted.
Kluz P.N., Kolb R., Xie Q., Borcherding N., Liu Q., Luo Y., Kim M.C., Wang L., Zhang Y., Li W., Stipp C., Gibson-Corley K.N., Zhao C., Qi H.H., Bellizzi A., Tao A.W., Sugg S., Weigel R.J., Zhou D., Shen X, & Zhang W. (2020). Cancer cell-intrinsic function of CD177 in attenuating β-Catenin signaling. Oncogene, 39(14), 2877-2889.
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2

Wnt Signaling Pathway Protein Expression

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The cells, normal mouse colon tissue and normal mouse skin tissue were lysed in RIPA buffer containing protease inhibitor cocktail (Sigma-Aldrich). The following antibodies were used: anti-β-actin (ab8226), anti-Wnt2b (ab178418), anti-Wnt3 (ab32249), anti-Wnt3a (ab28472), anti-Wnt4 (ab91226), anti-Wnt5a (ab72583), anti-Wnt7b (ab94915), anti-Wnt10a (ab106522), anti-FZD-5 (ab75234), and anti-FZD-7 (ab64635) were purchased from Abcam. Anti-active β-catenin (D13A1) and anti-total β-catenin (D10A8) were purchased from Cell Signaling Technology. Membranes were developed with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) and the optical density was analyzed using a Biospetrum 500 imaging system.
Sun X., Liu S., Wang D., Zhang Y., Li W., Guo Y., Zhang H, & Suo J. (2017). Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget, 8(9), 15168-15181.
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3

Immunohistochemical Analysis of Mammary Ducts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were processed with standard IHC protocols. High pH 9 (Vector Labs) was used for antigen retrieval and blocked with background punisher (BioCare Medical, Concord CA). Slides were incubated with primary antibody, anti Ki67 antibody (D2H10; Cell signaling), anti-KRT5 antibody (Poly 19055; Biolegend, San Diego, CA), anti-active β-catenin (D13A1; Cell Signaling), anti-ERα (C-311; Santa Cruz Biotechnology, Dallas, Texas), or anti-PR (D8Q2J; Cell Signaling) for 2 h. Next, rabbit or mouse-on-rodent polymers (BioCare Medical) for 30 min, developed with 3,3’-diaminobenzadine (DAB and 0.3% H2O2 in PBS) developing buffer, and counterstained. Positive cells and total cells per mammary duct were counted.
Kluz P.N., Kolb R., Xie Q., Borcherding N., Liu Q., Luo Y., Kim M.C., Wang L., Zhang Y., Li W., Stipp C., Gibson-Corley K.N., Zhao C., Qi H.H., Bellizzi A., Tao A.W., Sugg S., Weigel R.J., Zhou D., Shen X, & Zhang W. (2020). Cancer cell-intrinsic function of CD177 in attenuating β-Catenin signaling. Oncogene, 39(14), 2877-2889.
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