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Neb q5 high fidelity pcr mix

Manufactured by Qiagen
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The NEB Q5 High Fidelity PCR Mix is a ready-to-use solution for high-fidelity polymerase chain reaction (PCR) amplification. It contains a high-performance DNA polymerase that offers reliable and accurate DNA amplification.

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Lab products found in correlation

Tn5 transposase, by Illumina (2 mentions) Fragment analyzer system, by Agilent Technologies (2 mentions) Minelute pcr purification kit, by Qiagen (2 mentions)

2 protocols using neb q5 high fidelity pcr mix

1

Adapted ATAC-seq Protocol for Nuclei Isolation

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ATAC-seq was performed according to an adapted version adapted version of the Omni‐ATACseq protocol60 . Briefly, nuclei were isolated with nuclei isolation buffer (0.32 M sucrose, 3 mM CaCl2, 2 mM Mg Acetate, 0.1 mM EDTA, 10 mM Tris.HCl pH 8.0, 0.6% NP-40, 1mM DTT). After isolation, approximately 50,000 nuclei were resuspended in TD buffer with the Illumina Tn5 transposase (#15027865). The transposition reaction was performed at 37°C for 30 min. DNA fragments were purified from the reaction with the MinElute PCR purification Kit, Qiagen (# 28006) and amplified using the NEB Q5 High Fidelity PCR Mix (#M0492). Library quality was assessed using a Fragment Analyzer system (Agilent). ATAC-seq libraries were quantified using a KAPA library quantification kit and sequenced on a NovaSeq instrument in 50 bp paired‐end mode.
Haas L., Elewaut A., Gerard C.L., Umkehrer C., Leiendecker L., Pedersen M., Krecioch I., Hoffmann D., Novatchkova M., Kuttke M., Neumann T., de Silva I.P., Witthock H., Cuendet M.A., Carotta S., Harrington K.J., Zuber J., Scolyer R.A., Long G.V., Wilmott J.S., Michielin O., Vanharanta S., Wiesner T, & Obenauf A.C. (2021). Acquired resistance to anti-MAPK targeted therapy confers an immune-evasive tumour microenvironment and cross-resistance to immunotherapy in melanoma. Nature cancer, 2(7), 693-708.
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2

Adapted ATAC-seq Protocol for Nuclei Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols
ATAC-seq was performed according to an adapted version adapted version of the Omni‐ATACseq protocol60 . Briefly, nuclei were isolated with nuclei isolation buffer (0.32 M sucrose, 3 mM CaCl2, 2 mM Mg Acetate, 0.1 mM EDTA, 10 mM Tris.HCl pH 8.0, 0.6% NP-40, 1mM DTT). After isolation, approximately 50,000 nuclei were resuspended in TD buffer with the Illumina Tn5 transposase (#15027865). The transposition reaction was performed at 37°C for 30 min. DNA fragments were purified from the reaction with the MinElute PCR purification Kit, Qiagen (# 28006) and amplified using the NEB Q5 High Fidelity PCR Mix (#M0492). Library quality was assessed using a Fragment Analyzer system (Agilent). ATAC-seq libraries were quantified using a KAPA library quantification kit and sequenced on a NovaSeq instrument in 50 bp paired‐end mode.
Haas L., Elewaut A., Gerard C.L., Umkehrer C., Leiendecker L., Pedersen M., Krecioch I., Hoffmann D., Novatchkova M., Kuttke M., Neumann T., de Silva I.P., Witthock H., Cuendet M.A., Carotta S., Harrington K.J., Zuber J., Scolyer R.A., Long G.V., Wilmott J.S., Michielin O., Vanharanta S., Wiesner T, & Obenauf A.C. (2021). Acquired resistance to anti-MAPK targeted therapy confers an immune-evasive tumour microenvironment and cross-resistance to immunotherapy in melanoma. Nature cancer, 2(7), 693-708.
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