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C57bl 6j ly5

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C57BL/6J-Ly5.1 is a mouse strain that expresses the Ly5.1 allele, a marker used to distinguish cells derived from this strain. The Ly5.1 allele is a genetic variant of the Ptprc gene, which encodes the CD45 protein. This strain can be used as a tool in various immunological and hematopoietic studies.

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4 protocols using c57bl 6j ly5

1

Murine Hematopoietic Reconstitution Assay

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All mice were bred and maintained at the specific pathogen-free animal facilities of the Instituto Gulbenkian de Ciência (IGC, Oeiras, Portugal). C57BL/6J-Ly5.1 (C57BL/6J strain carrying the pan-leukocyte marker Ly5.1), C57BL/6J-Ly5.2 (C57BL/6J strain carrying the pan-leukocyte marker Ly5.2), and CAST/EiJ were originally received from The Jackson Laboratory (Bar Harbor, ME, United States). Animals used in reconstitution experiments were bred at our animal facility to generate female heterozygous F1 donor (CAST/EiJ × C57BL/6J-Ly5.2) and recipient (CAST/EiJ × C57BL/6J-Ly5.1) animals. Donor animals used in cell transfer experiments were <5 week-old and recipient animals were 5–16 week-old. This research project was reviewed and approved by the Ethics Committee of the IGC and by the Portuguese National Entity that regulates the use of laboratory animals.
Kubasova N., Alves-Pereira C.F., Gupta S., Vinogradova S., Gimelbrant A, & Barreto V.M. (2022). In Vivo Clonal Analysis Reveals Random Monoallelic Expression in Lymphocytes That Traces Back to Hematopoietic Stem Cells. Frontiers in Cell and Developmental Biology, 10, 827774.
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2

Genetically Modified Mouse Strains for Research

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C57BL/6J-Ly5.1, OT-I, UBC-GFP and Ai9 mice were obtained from Jackson Laboratories, and strains were maintained in the animal department of The Netherlands Cancer Institute (NKI). Ai9 and OT-I, and UBC-GFP and OT-I mice were crossed to obtain the Ai9;OT-I and GFP;OT-I strains, respectively. Between 5-10 mice, both male and female, of the age of 6 to 15 weeks were used for each experiment. All animal experiments were approved by the Animal Welfare Committee of the NKI, in accordance with national guidelines.
Bresser K., Kok L., Swain A.C., King L.A., Jacobs L., Weber T.S., Perié L., Duffy K.R., de Boer R.J., Scheeren F.A, & Schumacher T.N. (2022). Replicative history marks transcriptional and functional disparity in the CD8+ T cell memory pool. Nature immunology, 23(5), 791-801.
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Genetically Modified Mouse Strains

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C57BL/6J-Ly5.1, C57BL/6J, OT-I, mTmG, and UCB-GFP mice were obtained from Jackson Laboratory, and strains were maintained in the animal department of the Netherlands Cancer Institute. The mTmG and UCB-GFP mice were crossed with OT-I mice to obtain mTmG-OT-I and GFP-OT-I strains, respectively. All animal experiments were approved by the Animal Welfare Committee of the Netherlands Cancer Institute, in accordance with national guidelines.
Kok L., Dijkgraaf F.E., Urbanus J., Bresser K., Vredevoogd D.W., Cardoso R.F., Perié L., Beltman J.B, & Schumacher T.N. (2020). A committed tissue-resident memory T cell precursor within the circulating CD8+ effector T cell pool. The Journal of Experimental Medicine, 217(10), e20191711.
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4

Genetically Modified Mouse Strains for Research

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C57BL/6J-Ly5.1, OT-I, UBC-GFP and Ai9 mice were obtained from Jackson Laboratories, and strains were maintained in the animal department of The Netherlands Cancer Institute (NKI). Ai9 and OT-I, and UBC-GFP and OT-I mice were crossed to obtain the Ai9;OT-I and GFP;OT-I strains, respectively. Between 5-10 mice, both male and female, of the age of 6 to 15 weeks were used for each experiment. All animal experiments were approved by the Animal Welfare Committee of the NKI, in accordance with national guidelines.
Bresser K., Kok L., Swain A.C., King L.A., Jacobs L., Weber T.S., Perié L., Duffy K.R., de Boer R.J., Scheeren F.A, & Schumacher T.N. (2022). Replicative history marks transcriptional and functional disparity in the CD8+ T cell memory pool. Nature immunology, 23(5), 791-801.
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