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Cd48 apc cy7

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CD48-APC/Cy7 is a fluorescently-labeled antibody that binds to the CD48 cell surface antigen. CD48 is a glycosylphosphatidylinositol (GPI)-anchored cell surface molecule involved in cell-cell interactions. The APC/Cy7 fluorescent dye is conjugated to the antibody, allowing for detection and analysis using flow cytometry.

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Lab products found in correlation

F4 80 pe, by BioLegend (1 mentions) Cd45 af700, by BioLegend (1 mentions) Cd80 pe cy7, by BioLegend (1 mentions) Ly6c fitc, by BioLegend (1 mentions) Live dead fixable blue dead cell stain, by Thermo Fisher Scientific (1 mentions) Dylight 800, by Thermo Fisher Scientific (1 mentions) Cd11b buv395, by BD (1 mentions) B220 buv737, by BD (1 mentions) Mhcii 1 a 1 e bv510, by BioLegend (1 mentions) Cd4 bv570, by BioLegend (1 mentions) Cd11c bv785, by BioLegend (1 mentions) Siglec h percp cy5, by BioLegend (1 mentions) Cd3ε pe cy5, by Thermo Fisher Scientific (1 mentions) Ly6g biotin, by BioLegend (1 mentions) Cd8α buv805, by BD (1 mentions) Ccr2 apc, by R&D Systems (1 mentions) Nk1.1buv661, by BD (1 mentions) Cd34 fitc, by Thermo Fisher Scientific (1 mentions) Cd4 pe, by BioLegend (1 mentions) B220 apc, by BioLegend (1 mentions)

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APC-Cy7 (106 citations)

2 protocols using cd48 apc cy7

1

Multiparameter Flow Cytometry Immune Profiling

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Single-cell-suspensions of splenocytes and liver leukocytes were stained for cell surface markers using specific fluorophore-conjugated antibodies optimized for flow cytometry. Reagents and antibodies used in this study were LIVE/DEAD Fixable Blue Dead Cell Stain (ThermoFisher), CD11b-BUV395 (Clone: M1/70; BD Biosciences), Siglec-F-BUV615 (Clone: E50-2440; BD Biosciences), NK1.1-BUV661 (Clone: PK136; BD Biosciences), B220-BUV737 (Clone: RA3-6B2; BD Biosciences), CD8α-BUV805 (Clone: 53–6.7; BD Biosciences), MHCII(I-A/I-E)-BV510 (Clone: M5/114.15.2; BioLegend), CD4-BV570 (Clone: RM4-5; BioLegend), SCA-1-BV711 (Clone: D7; BioLegend), CD11c-BV785 (Clone: N418; BioLegend), Ly6C-FITC (Clone: HK1.4; BioLegend), Siglec-H-PerCP/Cy5.5 (Clone: 551; BioLegend), F4/80-PE (Clone: BM8; BioLegend), CD3ε-PE/Cy5 (Clone: 145-2C11; ThermoFisher), CD80-PE/Cy7 (Clone: 16-10A1; BioLegend), CD115-AF594 (Clone: AFS98; BioLegend), CCR2-APC (Clone: 475301; R&D Systems), CD45-AF700 (Clone: 30-F11; BioLegend), CD48-APC/Cy7 (Clone: HM48-1; BioLegend), Ly6G-Biotin (Clone: 1A8; BioLegend; with secondary antibody streptavidin-DyLight 800; ThermoFisher). Stained and fixed (4% PFA; 10 min in the dark) cells were analyzed with a Becton Dickson custom 10-laser ‘LSR-II’ flow cytometer and FlowJo software (v.10.4.1).
Jung S.R., Ashhurst T.M., West P.K., Viengkhou B., King N.J., Campbell I.L, & Hofer M.J. (2020). Contribution of STAT1 to innate and adaptive immunity during type I interferon-mediated lethal virus infection. PLoS Pathogens, 16(4), e1008525.
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2

Multicolor Flow Cytometry Panel

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The following antibodies were used: mouse lineage cocktail-PE (BioLegend, cat# 78035), mouse lineage cocktail-APC (R&D Systems, cat# FLC001A), c-Kit-FITC (BioLegend, cat# 161603), c-Kit-APC (BioLegend, cat# 135108), c-Kit-PE/Cy7 (BioLegend, cat# 105814), c-Kit-BV421 (BioLegend, cat# 135124), Sca-1-BV605 (BioLegend, cat# 108133), Sca-1-APC (eBioscience, cat# 17-5981-82), Sca-1-BV421 (BioLegend, cat# 108127), Sca-1-PerCP-Cy5.5 (eBioscience, cat# 45-5981-82), CD150-BV421 (BioLegend, cat# 115925), CD150-PE (eBioscience, cat# 12-1501-82), CD150-PE-Cy7 (BioLegend, cat# 115913), CD48-PE/Cy7 (eBioscience, cat# 25-0481-80), CD48-BV711 (BioLegend, cat# 103439), CD48-APC-Cy7 (BioLegend, cat# 103432),CD34-FITC (eBioscience, cat# 11-0341-82), CD135-PE-Cy5 (BioLegend, cat# 135311), CD135-APC (BioLegend, cat# 135310), CD45.1-PerCP-Cy5.5 (BioLegend, cat# 110727), CD45.2-BV421 (BioLegend, cat# 109831), CD45.2-FITC(eBioscience, cat# 11-0454-82), Gr1-PE (BioLegend, cat# 108407), CD11b-APC (BioLegend cat# 101211), CD4-PE (BioLegend,cat# 116005), CD8a-PE (BioLegend, cat# 100707), B220-APC (BioLegend, cat# 103211).
Wang S., Han J., Huang J., Islam K., Shi Y., Zhou Y., Kim D., Zhou J., Lian Z., Liu Y, & Huang J. (2024). Deep learning-based predictive classification of functional subpopulations of hematopoietic stem cells and multipotent progenitors. Stem Cell Research & Therapy, 15, 74.
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