LC-MS-grade methanol, 2-propanol, water, formic acid and HPLCgrade chloroform was purchased from Biosolve (Valkenswaard, The Netherlands). LC-MS grade ammonium formate and sodium hydroxide were from Sigma-Aldrich Chemie GmbH (St Louis, USA), butanol and hydrochloric acid were from Merck Millipore (Billerica, USA). C18-lysoSM and C18-lysoGb3 were from Avanti (Albaster, USA). 13 C 5 -sphinganine, 13 C 5 -sphingosine, 13 C 5 -GlcSph and 13 C 5 -lysoGb3 were synthesized at the Department of Bio-organic Synthesis of the Leiden Institute of Chemistry, Leiden University [10] . The label was incorporated in C5, 6, 7, 8, and 9 of the sphingosine backbone (see Fig. 1). C18-sphingosine, C18-sphinganine, C18-GlcSph, C18lactosylsphingosine and C17-lysoSM were obtained from Avanti. C17-dihydroceramide (C17-dhCer) was synthesized in house (see Scheme 1); C17-sphinganine, C18-ceramide (Cer), C18glucosylceramide (GlcCer) and Lactosylceramide (LacCer) were purchased from Avanti and C18-globotriaosylceramide (Gb3) from Matreya (State College, USA).
Hplc grade chloroform
Manufactured by Biosolve
Sourced in Netherlands
HPLC grade chloroform is a high-purity solvent used in analytical and laboratory applications. It is a colorless, volatile liquid with a characteristic odor. The product meets the specifications for use in high-performance liquid chromatography (HPLC) and other analytical techniques that require a consistent, high-quality solvent.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Biosolve (2 mentions)
Lc ms grade ammonium formate, by Merck Group (2 mentions)
Lc ms grade methanol, by Biosolve (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
2 propanol, by Biosolve (2 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Avantor (1 mentions)
Acetic acid, by Avantor (1 mentions)
Ammonium hydroxide, by Thermo Fisher Scientific (1 mentions)
Milli q advantage a10 water purification system, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Laccer, by Avanti Polar Lipids (1 mentions)
Dhcer, by Avanti Polar Lipids (1 mentions)
Glccer, by Avanti Polar Lipids (1 mentions)
Galactosylceramide galcer, by Avanti Polar Lipids (1 mentions)
Acetonitrile, by Biosolve (1 mentions)
Millipore water, by Merck Group (1 mentions)
Direct q3, by Merck Group (1 mentions)
4 protocols using hplc grade chloroform
1
Comprehensive Lipidomics Profiling Protocol
2
Isotope-Labeled Amino Acid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC grade methanol and acetonitrile were obtained from Actu‐All Chemicals (Oss, the Netherlands). HPLC grade chloroform was provided by Biosolve Chemicals (Valkensweerd, the Netherlands). Acetic acid (99–100%) and sodium hydroxide were purchased from VWR (Amsterdam, the Netherlands). Ammonium hydroxide (28–30%) was acquired from Acros Organics (Amsterdam, the Netherlands). Water in this work was produced by a Milli‐Q® Advantage A10 Water Purification System from Millipore (Amsterdam‐Zuidoost, the Netherlands). The standards of eleven 13C, 15N and/or D‐isotope‐labeled amino acids were purchased from Cambridge Isotope Laboratories (Apeldoorn, the Netherlands). In Study I, DL‐phenyl‐D5‐alanine from CDN ISOTOPES (Nieuwegein, the Netherlands) was used as the internal standard (IS). In study II, an L‐methionine sulfone‐containing solution from Human Metabolome Technologies (Leiden, the Netherlands) was employed as IS. All compounds were dissolved in a mixture of water:acetonitrile (95:5, containing 0.5% v/v formic acid) and subsequently diluted to desired concentrations with water (see Tables 1 and 2 ). A solution of Acetic acid (10% v/v in water, pH = 2.2) was employed as BGE.
3
Synthesis of Lipid Standards for Gaucher's Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GCase specific inhibitor (ME656) (34 (link)), 13C5-sphinganine, 13C5-sphingosine, 13C5-GlcSph, 13C5-lyso-globotriaosylceramide (LysoGb3), C17-lysosphingomyelin (LysoSM), 13C6-GlcChol, and C17-dihydroceramide (38 (link), 39 (link)) were synthesized as reported. All chemicals and reagents were obtained from Sigma-Aldrich (St Louis, USA) unless mentioned otherwise. The standards Cer (d18:1/16:0), dhCer (d18:0/16:0), GlcCer (d18:1/16:0), galactosylceramide (GalCer) (d18:1/16:0), and LacCer (d18:1/16:0) were obtained from Avanti Polar lipids (Alabaster, USA) and glucosylated cholesterol (GlcChol) from Sigma-Aldrich. LC-MS grade methanol, 2-propanol, water, formic acid, acetonitrile, and HPLC grade chloroform were purchased from Biosolve (Valkenswaard, the Netherlands). LC-MS grade ammonium formate, ammonium acetate, and sodium hydroxide from Sigma-Aldrich, and butanol and hydrochloric acid from Merck Millipore (Billerica, USA).
4
Octadecyl Phosphonic Acid Surface Functionalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Octadecyl phosphonic acid was purchased from TCI Chemicals. HPLC-grade chloroform stabilized with ethanol was purchased from Biosolve and used without further purification. Millipore water was obtained from Direct-Q 3 Millipore (Merck, Germany). Surfactant 1 was prepared according to previously published procedure18 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!