Alexa fluor 488

Manufactured by Southern Biotech

Sourced in United States

Alexa Fluor 488 is a fluorescent dye that can be used to label proteins, cells, and other biological molecules. It has an excitation maximum at 488 nm and an emission maximum at 520 nm, making it compatible with common fluorescence microscopy and flow cytometry equipment.

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Lab products found in correlation

Ab20459, by Abcam (1 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions) Talent qpcr premix sybr green kit, by Tiangen Biotech (1 mentions) Taq pcr mix, by Tiangen Biotech (1 mentions) Goat anti rabbit, by Southern Biotech (1 mentions) Columbia blood agar base, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Mueller hinton agar, by Thermo Fisher Scientific (1 mentions) Saponin, by Merck Group (1 mentions) Tianamp bacteria dna kit, by Tiangen Biotech (1 mentions) Fastking gdna dispelling rt supermix kit, by Tiangen Biotech (1 mentions) Lsrii instrument, by BD (1 mentions) Streptavidin allophycocyanin, by Thermo Fisher Scientific (1 mentions) Donkey serum, by Southern Biotech (1 mentions) Donkey serum, by GE Healthcare (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 596, by Thermo Fisher Scientific (1 mentions) Fluoromount g, by Southern Biotech (1 mentions)

3 protocols using alexa fluor 488

Helicobacter pylori Culture and Characterization

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Columbia blood agar base, brain heart infusion (BHI), Mueller–Hinton (MH) agar, and H. pylori selective supplement (Dent) SR0147E were obtained from Oxoid, United Kingdom. Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS), Hank’s balanced salt solution (HBSS), MEM nonessential amino acids, TRIzol reagent, and Live/Dead BacLight Bacterial Viability kits (Molecular Probes) were purchased from Thermo Fisher Scientific, United States. Sheep blood was procured from Pingrui Biotechnology, China. CLR was purchased from Dalian Meilun Biotechnology, China. MTZ and saponin were acquired from Sigma, Germany. Fastking gDNA Dispelling RT SuperMix Kit, Talent qPCR PreMix (SYBR Green) Kit, TIANamp Bacteria DNA kit, and 2 × Taq PCR Mix were purchased from TIANGEN, China. Anti-H. pylori antibody ab20459 was obtained from Abcam, United Kingdom. Alexa Fluor 488 and goat anti-rabbit were bought from Southern Biotech, United States.

Zhong Y., Tang L., Deng Q., Jing L., Zhang J., Zhang Y., Yu F., Ou Y., Guo S., Huang B., Cao H., Huang P, & Xu Y. (2021). Unraveling the Novel Effect of Patchouli Alcohol Against the Antibiotic Resistance of Helicobacter pylori. Frontiers in Microbiology, 12, 674560.

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Flow Cytometric Analysis of Shark Immunoglobulins

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Thymocytes and splenocytes (5 × 10⁵ cells per treatment) were stained with either biotinylated mouse mAb LK14 (48 ) (against G. cirratum IgL) or unlabeled anti-IgHV rabbit polyclonal (against G. cirratum IgMV1, IgWV1, or IgWV2) at 1:100 in staining buffer (1% BSA in shark PBS) for 1 h at 4°C. Cells were then washed three times with staining buffer before staining with streptavidin-allophycocyanin (eBioscience, San Diego, CA) at 1:1500 and anti-rabbit Alexa Fluor 488 at 1:500 (Southern Biotech, Birmingham, AL), respectively, for 30 min at 4°C. All samples were washed and resuspended in 300 μl of staining buffer containing 0.1% sodium azide and examined by flow cytometry on a BD LSR II instrument (BD Biosciences, San Jose, CA). Fifty thousand events were collected, gated for live cells, and analyzed using the FlowJo software (Tree Star, Ashland, OR). Identical signal thresholds could be applied to all samples except the 120-mo shark, which had lower fluorescent intensity across all experiments.

Deiss T.C., Breaux B., Ott J.A., Daniel R.A., Chen P.L., Castro C.D., Ohta Y., Flajnik M.F, & Criscitiello M.F. (2019). Ancient Use of Ig Variable Domains Contributes Significantly to the TCRδ Repertoire. Journal of immunology (Baltimore, Md. : 1950), 203(5), 1265-1275.

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Evaluating Graft Survival and Lesions

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Double/triple immunofluorescence labeling techniques were performed with direct-mounted sections to assess graft survival and lesion extent in vivo. Sections were washed in TBS, blocked in TBS/5% donkey serum (GE Healthcare)/0.25% Triton X-100 (Neolab) for 1 h and incubated with primary antibodies in TBS/1% donkey serum/0.25% Triton X-100 overnight at 4°C. The following day, sections were rinsed in TBS/1% donkey serum and incubated with Alexa Fluor 594 or Alexa Fluor 488 donkey secondary antibodies for 2 h. Sections were coverslipped with Fluoromount G (Southern Biotech, Birmingham, AL, USA).
The following primary antibodies were used: mouse anti-GFAP for astroglia (1:1,000; Merck Millipore), rabbit anti-GFP (green fluorescent protein, 1:750; Invitrogen) to detect the grafted cells, rabbit anti-Ki-67 (1:500; Novacastra/Leica Biosystems Wetzlar, Germany) for proliferating cells, and DAPI as nuclear counterstain. Immunolabeling was visualized using Alexa Fluor 488 (1:300; Life Technologies) or Alexa Fluor 596 (1:300; Life Technologies)-linked donkey secondary antibodies.

Sandner B., Ciatipis M., Motsch M., Soljanik I., Weidner N, & Blesch A. (2016). Limited Functional Effects of Subacute Syngeneic Bone Marrow Stromal Cell Transplantation After Rat Spinal Cord Contusion Injury. Cell transplantation, 25(1).

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