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10 ncp

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10-NCP is a lab equipment product. It serves as a core function, but no further details are available to provide an unbiased and factual description without extrapolation.

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Lab products found in correlation

Calpeptin, by Merck Group (1 mentions) Riluzole, by Merck Group (1 mentions) Rapamycin, by Cell Signaling Technology (1 mentions) Bafilomycin a, by Merck Group (1 mentions) Nutlin 3, by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) 3 methyladenine, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Salubrinal, by Santa Cruz Biotechnology (1 mentions) 4 phenylbutyric acid 4 pba, by Merck Group (1 mentions) Thapsigargin, by Merck Group (1 mentions) Tunicamycin, by Merck Group (1 mentions)

2 protocols using 10 ncp

1

Pharmacological Modulation of Motor Neurons

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Motor neurons were treated with various drugs. The drugs used in this study are TTX (T8024, Sigma), riluzole (R116, Sigma), 3-methyladenine (3-MA, M9281, Sigma), MG132 (M7449, Sigma), nutlin3 (N6287, Sigma), calpeptin (C8999, Sigma) and bafilomycin A (B1793, Sigma), N-methyl D-aspartate (NMDA, Cay-14581, Biomol), rapamycin (#9904, Cell Signaling), Torin-1 (#CALB475991, Calbiochem), 10-NCP (#2338, Biovision) and cycloheximide (#01810, Sigma). Concentrations of drugs used in this study can be found in the figure legends.
Gonçalves I.D., Brecht J., Thelen M.P., Rehorst W.A., Peters M., Lee H.J., Motameny S., Torres-Benito L., Ebrahimi-Fakhari D., Kononenko N.L., Altmüller J., Vilchez D., Sahin M., Wirth B, & Kye M.J. (2018). Neuronal activity regulates DROSHA via autophagy in spinal muscular atrophy. Scientific Reports, 8, 7907.
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2

Modulating ER Stress and Autophagy in Neuronal Viability

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To determine the influence of ER stress on neuronal viability following OGD, pharmacological agents that can modulate ER stress were investigated. Thapsigargin (Sigma) (0.01-10μM) and tunicamycin (Sigma) (0.01-1μM) were used to induce ER stress, while salubrinal (Santa Cruz Biotechnology) (25-500µM) and 4-Phenylbutyric acid (4-PBA) (Sigma) (10μM-10mM) were used to inhibit ER stress. To investigate modulation of autophagy, 10-NCP (BioVision) (0.1-10μM) was used to induce autophagy and 3MA (Sigma) (0.1-10μM) was used to inhibit autophagy.
All drugs were administered during the 2 h OGD/normoxia period. The vehicle control was either media alone or 2% DMSO in OGD/normoxia media.
Hadley G., Neuhaus A.A., Couch Y., Beard D.J., Adriaanse B.A., Vekrellis K., DeLuca G.C., Papadakis M., Sutherland B.A, & Buchan A.M. (2018). The role of the endoplasmic reticulum stress response following cerebral ischemia. International journal of stroke : official journal of the International Stroke Society, 13(4).
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