Gzmb fitc

Manufactured by BioLegend

The GZMB-FITC product is a fluorescently labeled antibody that specifically binds to the Granzyme B protein. Granzyme B is a serine protease found in the cytotoxic granules of natural killer cells and cytotoxic T lymphocytes and plays a crucial role in cell-mediated apoptosis. The FITC (Fluorescein Isothiocyanate) label allows for the detection and analysis of Granzyme B-expressing cells using flow cytometry or other fluorescence-based techniques.

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FITC anti-human/mouse GZMB (2 citations)

2 protocols using gzmb fitc

Intracellular Flow Cytometry of CD8+ T Cells

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CD8⁺ T cells were cultured for 1 h in complete RPMI in the presence of brefeldin A and subsequently stained for viability using the viability dyes eFluor 506, 520, or 780 (eBioscience), according to the provided protocol. After surface marker staining, cells were permeabilized and fixed using the Foxp3/Transcription Factor Fixation/Permeabilization Concentrate and Diluent kit (eBioscience). Intracellular GZMB-FITC (515403; BioLegend), FoxO1-PE (14262; Cell Signaling Technology), and isotope control (5742; Cell Signaling Technology) staining was performed in FOXP3 perm/wash buffer for 1 h at 4°C. After washing and fixation of the cells in 1% paraformaldehyde/PBS, cells were analyzed by flow cytometry.

Jeng M.Y., Hull P.A., Fei M., Kwon H.S., Tsou C.L., Kasler H., Ng C.P., Gordon D.E., Johnson J., Krogan N., Verdin E, & Ott M. (2018). Metabolic reprogramming of human CD8+ memory T cells through loss of SIRT1. The Journal of Experimental Medicine, 215(1), 51-62.

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Multiparameter Flow Cytometry Panel

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PD-1 FITC (1:300; J43; eBioscience), NK1.1 PE (1:300; PK136; eBioscience), CD3e PE-Dazzle594 (1:300; 145-2C11; BioLegend), CD3e PE (1:200; 145-2C11; eBioscience), FoxP3 PerCP-Cy5.5 (1:200; FJK-16s; eBioscience), CD8a PE-Cy7 (1:2000; 53-6.7; eBioscience), CD8a APC (1:600; 53-6.7; eBioscience), CD8a PerCP-Cy5.5 (1:400; 53-6.7; eBioscience), TCRb AF700 (1:100; H57-597; BioLegend), CD45 BV785 (1:1000; 30-F11; BioLegend), CD4 BV711 (1:400; GK1.5; BioLegend), CD19 BV650 (1:400; 6D5; BioLegend), CD19 PE (1:500; 1D3; eBioscience), CD11b BV605 (1:1000; M1/70; BioLegend), CD11b BV510 (1:400; M1/70; BioLegend), CD11c BV605 (1:400; N418; BioLegend), Siglec-F PE (1:300; E50-2440; BD Biosciences), F4/80 APC (1:200; BM8; BioLegend), Ly-6G AF 700 (1:300; 1A8; BioLegend), MHC II BV650 (1:4000; M5/114.15.2; BioLegend), CD64 BV421 (1:200; X54-5/7.1; BioLegend), TNF FITC (1:300; MP6-XT22; BioLegend), IFNg PE-Cy7 (1: 1000; XMG1.2; BD Biosciences), Eomes PE (1:200; W17001A; BioLegend), Ki-67 BV 650 (1:200, 11F6; Biolegend), GzmB FITC (1:100; GB11; BioLegend). Gating strategy is depicted in Supplementary Fig. 6d.

Frey N., Tortola L., Egli D., Janjuha S., Rothgangl T., Marquart K.F., Ampenberger F., Kopf M, & Schwank G. (2022). Loss of Rnf31 and Vps4b sensitizes pancreatic cancer to T cell-mediated killing. Nature Communications, 13, 1804.

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